菊花茎叶清除自由基动力学特性及抗氧化活性成分的在线筛选

目的本研究对菊花茎叶抗氧化活性和清除自由基动力学特性进行考察,在线筛选与清除自由基相关的活性成分。方法采用DPPH法和ABTS法观察菊花茎叶提取物的抗氧化活性,比较了样品浓度、反应温度和反应时间等影响因素对自由基清除率的影响,测定了菊花茎叶清除自由基活性的半数清除率(IC50)。以木犀草素葡萄糖醛酸苷、木犀草苷和咖啡酸等黄酮成分、绿原酸、新绿原酸、隐绿原酸、异绿原酸A、B、C等酚酸成分为指标,采用HPLC法对反应前后菊花茎叶进行了含量测定,计算上述成分的消耗率。结果菊花茎叶的样品浓度、反应温度和反应时间均对其对清除DPPH自由基和ABTS自由基清除率有影响;菊花茎叶清除DPPH和ABTS自由基的IC50分别为0.395 mg·mL^(-1)、2.039 mg·mL^(-1)。与DPPH和ABTS自由基反应后,菊花茎叶样品中上述测定成分的含量均明显下降,推测上述成分可能是菊花茎叶抗氧化的活性成分。结论菊花茎叶具有较好的清除自由基活性,本研究初步明确其动力学特性,在线筛选出了抗氧化活性成分,为今后菊花茎叶的综合开发提供实验依据。

菊花茎叶化学成分及其抗菌抗氧化活性研究进展

综述了菊花茎叶化学成分及其抗菌、抗氧化活性研究进展,为进一步研究提供科学依据。参考文献27篇。

Construction of Highly-efficient Direct Regeneration System of Different Chrysanthemum Species

Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and re- generation coefficient at 2.3-9.4. However, none adventitious buds were found differ- entiated from leaves of ＂28＂ and ＂5-17＂, and leaf regeneration capacity of ＂11＂ was lower, either. Most chrysanthemum species proved higher in stem regeneration ca- pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.