期刊文献+
共找到12篇文章
< 1 >
每页显示 20 50 100
初始pH值对不同灵芝深层发酵菌体多糖的影响 被引量:2
1
作者 曾东方 张小朋 +1 位作者 罗雪莲 吴元欣 《化学与生物工程》 CAS 2004年第5期19-21,共3页
研究了发酵初期pH4 0~5 4范围内的调节与设定对赤芝、紫芝、甜芝等3种不同物种灵芝菌体发酵以及主要药用生物活性成分多糖发酵的影响,结果表明:赤芝、紫芝菌体发酵的初始条件以pH4 3最适,而甜芝以pH4 8最适;紫芝胞外多糖发酵在初始条... 研究了发酵初期pH4 0~5 4范围内的调节与设定对赤芝、紫芝、甜芝等3种不同物种灵芝菌体发酵以及主要药用生物活性成分多糖发酵的影响,结果表明:赤芝、紫芝菌体发酵的初始条件以pH4 3最适,而甜芝以pH4 8最适;紫芝胞外多糖发酵在初始条件设为pH5 4时产生最多,而赤芝、甜芝胞外多糖的发酵产量未受初始pH试验范围的影响;赤芝、紫芝在初始pH4 8条件下发酵胞内多糖积累最少,而甜芝此条件下发酵胞内多糖积累最多。讨论认为不同物种灵芝以菌体或者多糖发酵为目标进行优化控制,其发酵初期pH尚需调节到相应的最适设定值。 展开更多
关键词 灵芝物种 摇瓶发酵 菌体多糖 初始PH值 优化控制
下载PDF
双歧杆菌、大肠杆菌菌体多糖对iIEL激活作用的对比研究 被引量:2
2
作者 王舰 王莉莉 +3 位作者 张京航 周正任 杨晓临 王丹 《微生物学杂志》 CAS CSCD 2000年第1期8-10,共3页
采用Percoll非连续密度梯度离心法从小鼠小肠提取小肠上皮间淋巴细胞(intestinalintraeptheliallymphocyte,iIEL),应用双抗原标记间接免疫荧光法对其抗原表型进行测定;同时,应用有机溶剂提取的双歧杆菌、大肠杆菌菌体多糖与iIEL... 采用Percoll非连续密度梯度离心法从小鼠小肠提取小肠上皮间淋巴细胞(intestinalintraeptheliallymphocyte,iIEL),应用双抗原标记间接免疫荧光法对其抗原表型进行测定;同时,应用有机溶剂提取的双歧杆菌、大肠杆菌菌体多糖与iIEL细胞共同孵育作用后,检测iIEL细胞的自然杀伤活性、IFNγ和IL-2产生能力。结果表明:iIEL细胞的表型为85.1%CD3+73.8%CD8+细胞,且有TCRγδ表达。双歧杆菌、大肠杆菌菌体多糖均能增加iIEL细胞的自然杀伤活性,并能促进iIEL细胞产生IFNγ和IL-2但双歧杆菌菌体多糖的作用强。 展开更多
关键词 双歧杆菌体多糖 iIEL 激活作用 免疫机制
下载PDF
液体发酵蜜环菌培养条件的优化及菌体多糖的分离 被引量:9
3
作者 张颖 沈业寿 《食用菌》 2001年第2期5-7,共3页
以四因素三水平的正交实验设计培养基中碳源、氮源浓度,对蜜环菌进行液体发酵,通过对生长的菌丝体于重的测定,得到该菌株生长最适培养基配方,并证明氮源为其生长的最重要因素,同时对该菌株的培养优化条件进行了研究,并提取蜜环菌... 以四因素三水平的正交实验设计培养基中碳源、氮源浓度,对蜜环菌进行液体发酵,通过对生长的菌丝体于重的测定,得到该菌株生长最适培养基配方,并证明氮源为其生长的最重要因素,同时对该菌株的培养优化条件进行了研究,并提取蜜环菌菌体多糖,发现液体发酵菌体的多糖含量远高于人工栽培的蜜环菌菌索。 展开更多
关键词 蜜环 培养条件 发酵 菌体多糖 分离 培养基
下载PDF
双歧杆菌菌体多糖对iIEL细胞产生IFN_γ和IL-2激活作用的研究
4
作者 王舰 周正任 +2 位作者 杨晓临 王丹 何丹江 《中国公共卫生》 CAS CSCD 北大核心 2001年第6期519-520,共2页
目的 检测小肠上皮间淋巴细胞 (iIEL)产生IFNγ 和IL - 2的能力。方法 采用Percoll非连续密度梯度离心法从小鼠小肠提取iIEL细胞 ,应用有机溶剂提取的双歧杆菌菌体多糖 ,将它与iIEL细胞共同孵育作用。结果 双岐杆菌多糖组与正常对照... 目的 检测小肠上皮间淋巴细胞 (iIEL)产生IFNγ 和IL - 2的能力。方法 采用Percoll非连续密度梯度离心法从小鼠小肠提取iIEL细胞 ,应用有机溶剂提取的双歧杆菌菌体多糖 ,将它与iIEL细胞共同孵育作用。结果 双岐杆菌多糖组与正常对照组之间有显著差异 (P <0 0 1)。结论 双歧杆菌菌体多糖能促进iIEL细胞产生IFNγ 和IL 2。 展开更多
关键词 双歧杆菌体多糖 小肠上皮间淋巴细胞 IFNΓ IL-2
下载PDF
双歧杆菌、大肠杆菌菌体外多糖对iIEL激活作用的对比研究 被引量:4
5
作者 周正任 王舰 +1 位作者 杨晓临 王丹 《中国免疫学杂志》 CAS CSCD 北大核心 2001年第6期296-298,301,共4页
目的 :对比研究双歧杆菌、大肠杆菌菌体多糖对iIEL的激活作用。方法 :采用Percoll非连续密度梯度离心法从小鼠小肠提取小肠上皮间淋巴细胞 (iIEL) ;应用有机溶剂提取的双歧杆菌、大肠杆菌菌体多糖与iIEL细胞共同孵育作用后 ,检测iIEL细... 目的 :对比研究双歧杆菌、大肠杆菌菌体多糖对iIEL的激活作用。方法 :采用Percoll非连续密度梯度离心法从小鼠小肠提取小肠上皮间淋巴细胞 (iIEL) ;应用有机溶剂提取的双歧杆菌、大肠杆菌菌体多糖与iIEL细胞共同孵育作用后 ,检测iIEL细胞的自然杀伤活性、IFNγ和IL 2产生能力。结果 :大肠杆菌、双歧杆菌菌体外多糖均能显著增强iIEL细胞的自然杀伤活性、IFNγ和IL 2的产生能力 (P <0 0 1) ,双歧杆菌组的作用显著高于大肠杆菌组 (P <0 0 1)。结论 :双歧杆菌和大肠杆菌菌体外多糖均对iIEL细胞具有免疫激活作用 ,但双歧杆菌菌体多糖的作用强。 展开更多
关键词 双歧杆多糖 小肠上皮间淋巴细胞 IFNΓ IL-2 iIEL 免疫激活作用 双歧杆 大肠杆
下载PDF
一株韭菜内生高效氯氰菊酯降解生防细菌的分离与鉴定 被引量:6
6
作者 任明 赵蕾 《西北农业学报》 CAS CSCD 北大核心 2010年第3期57-61,共5页
从新鲜韭菜的根中分离到一株对多种蔬菜病原真菌有抑制作用的优势内生细菌W7,对其拮抗机制的初步研究结果表明,胞外代谢物对病原真菌无抑制作用,而菌体经超声波破碎及有机溶剂沉淀得到的多糖粗提液可明显抑制病原菌菌丝生长。该菌还能以... 从新鲜韭菜的根中分离到一株对多种蔬菜病原真菌有抑制作用的优势内生细菌W7,对其拮抗机制的初步研究结果表明,胞外代谢物对病原真菌无抑制作用,而菌体经超声波破碎及有机溶剂沉淀得到的多糖粗提液可明显抑制病原菌菌丝生长。该菌还能以100 mg/L高效氯氰菊酯为唯一碳源生长,7 d的降解率为51.3%,表明该菌是一株兼具农药降解特性的生防内生细菌。通过对其形态特征、生理生化及16S rDNA同源性序列分析,初步鉴定该菌为类芽孢杆菌(Paenibacillus spp.)。 展开更多
关键词 内生细 拮抗 菌体多糖 高效氯氰菊酯 类芽孢杆
下载PDF
Production and characterization of exopolysaccharides in mycelial culture of Cordyceps sinensis fungus Cs-HK1 with different carbon sources 被引量:4
7
作者 Xia Chen Jian-yong Wu Xiaoting Gui 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2016年第1期158-162,共5页
The effects of different carbon sources(sugars) on the production and molecular properties of exopolysaccharides(EPS) were evaluated in the mycelial liquid culture of a medicinal fungus Cordyceps sinensis Cs-HK1. Gala... The effects of different carbon sources(sugars) on the production and molecular properties of exopolysaccharides(EPS) were evaluated in the mycelial liquid culture of a medicinal fungus Cordyceps sinensis Cs-HK1. Galactose or mannose was used(at 5 g·L^(-1)) as a secondary carbon source with glucose(35 g·L^(-1)) at the mass ratio of 1:7. Mannose was consumed notably since the first day of culture, but galactose was not even after glucose was exhausted.The volumetric yield of EPS in culture was increased slightly with the addition of galactose and decreased with mannose. The monosaccharide composition of EPS was also different, e.g., on day 8, the glucose contents of EPS were 76%with the addition of mannose, 59% with galactose, compared with 62% with glucose only. The molecular weight distribution of EPS was also affected by the secondary carbon source, being generally lower compared with that with glucose only. The results suggested that the addition of galactose improved the total yield of EPS in culture while mannose can improve the yield of glucan constituent of EPS. 展开更多
关键词 Cordyceps sinensis Fermentation Carbon source Hydrocarbons Bioprocess Monosaccharide composition
下载PDF
Study of the Action Mode of Wickerhamomyces anomalus against Colletotrichum gloeosporioides
8
作者 Luis Femando Zepeda-Giraud Dario Rafael Olicon-Hemandez +1 位作者 Cinthya Martinez-Lopez Guadalupe Guerra-Sanchez 《Journal of Agricultural Science and Technology(B)》 2016年第5期341-349,共9页
Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide va... Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide variety of products. In this work, the antifungal activity of Wickerhamomyces anomalus against C. gloeosporioides isolated from contaminated avocados was evaluated. The antagonism and volatile compound inhibition were measured on Petri dishes. In the mixed cultures, the mycelia damage was observed by scanning electron microscope (SEM). Chitinase and glucanase production by the antagonism was quantified by the reducing sugars method, and biofilm formation was evaluated with 1% crystal violet. The yeast W. anomalus could reduce the growth of C. gloeosporioides up to 65% by direct antagonism and 10% by volatile compounds. The antagonist did not allow the conidia germination and mycelia growth in any of the tested formulations. SEM showed mycelial damage caused by W. anomalus. The antagonist showed adhesion to the mycelium by a polysaccharide biofilm. The presence of mycelium stimulated the hydrolytic enzyme production with the maximal activity of 21.4 U/mg for chitinases at 24 h and 10 U/mg for glucanases at 60 h. These results showed that W. anomalus used together different mechanisms to express its antifungal activity against C. gloeosporioides. This study might be the first report for this phytopathogen isolated from avocado fruits, which could represent an opportunity to establish biocontrol of diseases for this agricultural product. 展开更多
关键词 Colletotrichum gloeosporioides Wickerhamomyces anomalus BIOCONTROL AVOCADO
下载PDF
Role of calcium signaling in down-regulation of aggrecan induced by cyclic tensile strain in annulus fibrosus cells
9
作者 郭志良 周跃 +2 位作者 李华壮 曹国永 滕海军 《Journal of Medical Colleges of PLA(China)》 CAS 2006年第6期379-382,共4页
Objective:To study the role of intracellular calcium signal pathway in the down-regulation of aggrecan induced by cyclic tensile strain in the annulus fibrosus cells. Methods:The expression of aggrecan mRNA and core p... Objective:To study the role of intracellular calcium signal pathway in the down-regulation of aggrecan induced by cyclic tensile strain in the annulus fibrosus cells. Methods:The expression of aggrecan mRNA and core protein were respectively detected with RT-PCR and western blot after the channels transmitting calcium ions were blocked with EGTA, gadolinium and verapamil. Results :EGTA. gadolinium and verapamil partially prevented the effects of cyclic tensile strain on the expression of aggrecan in annulus fibrosus cells. Conclusion:The calcium signaling is involved in the down-regulation of proteoglycan resulting from cyclic tensile strain in the annulus fibrosus cells. 展开更多
关键词 annulus fibrosus cells calcium ion PROTEOGLYCAN
下载PDF
嗜水气单胞菌对水生动物的致病作用及防治 被引量:4
10
作者 冯守明 《天津水产》 2002年第1期19-23,共5页
嗜水气单胞菌(Aeromonas hydrophila)导致水产动物患病的报道最早见于1891年,但由于在自然界中尤其在水中该菌广泛分布,一般为共栖菌,因此,一直未对其危害性充分重视。近年来,由于水产养殖环境的恶化,导致该类水产疾病大范围流行,造成... 嗜水气单胞菌(Aeromonas hydrophila)导致水产动物患病的报道最早见于1891年,但由于在自然界中尤其在水中该菌广泛分布,一般为共栖菌,因此,一直未对其危害性充分重视。近年来,由于水产养殖环境的恶化,导致该类水产疾病大范围流行,造成很大危害,相关的研究报道很多,涉及病原、寄主、病理、流行病学及防治技术等多个方面,现就上述内容综述如下。 展开更多
关键词 嗜水气单胞 水产养殖环境 AEROMONAS 共栖 能力 多糖 免疫方法 人工感染试验 非致病 败血
原文传递
Effect of Inonotus Obliquus Polysaccharides on physical fatigue in mice 被引量:4
11
作者 Zhong Yue Zhong Xiuhong +1 位作者 Yang Shuyan Zheng Zhonghua 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2015年第4期468-472,共5页
OBJECTIVE: To evaluate the potential beneficial effects of Inonotus obliquuspolysaccharides(IOP) on the alleviation of physical fatigue in mice.METHODS: Sixty-four male mice were randomly divided into four groups(n = ... OBJECTIVE: To evaluate the potential beneficial effects of Inonotus obliquuspolysaccharides(IOP) on the alleviation of physical fatigue in mice.METHODS: Sixty-four male mice were randomly divided into four groups(n = 16 per group). Mice were orally administered IOP for a period of 14 days at 0,100,200 and 300 mg/kg/d,and were assigned to the control,IOP-100,IOP-200,and IOP-300 groups,respectively by the random number table method. Mice in the control group received an oral administration of sterile distilled water. A forced swimming test was performed for 8 mice per group at one hour after the last treatment. The other 8mice in each group swam for 30 min. Blood,liver and muscle samples were taken after resting for30 min. Levels of blood urea nitrogen and lactate,as well as glycogen contents of the liver and muscle were measured. Morphology of liver was observed by light microscopy.RESULTS: IOP extended the swimming time of mice,and increased the glycogen content of liver and muscle,but decreased blood lactic acid and serum urea nitrogen levels. IOP had no toxic effects on major organs such as the liver as assessed by histopathological examinations.CONCLUSION: IOP might be a potential anti-fatigue pharmacological agent. 展开更多
关键词 Polysaccharides Fatigue Blood urea ni-trogen Lactic Acid Glycogen Anatomy and histolo-gy Pharmacological phenomena
原文传递
浅议我国水产养殖动物病害的防控措施
12
作者 卓德武 《天津水产》 2011年第Z1期12-15,共4页
随着水产养殖业的不断发展,养殖产量大幅度提高,人们对水产品的需求得到进一步满足。与此同时,水生动物赖以生存的水环境污染问题日益突出,养殖池塘水体进一步老化,由此导致水产养殖动物病害日趋严重。新的病害不断涌现,有的已呈暴发性... 随着水产养殖业的不断发展,养殖产量大幅度提高,人们对水产品的需求得到进一步满足。与此同时,水生动物赖以生存的水环境污染问题日益突出,养殖池塘水体进一步老化,由此导致水产养殖动物病害日趋严重。新的病害不断涌现,有的已呈暴发性趋势。 展开更多
关键词 水产养殖动物 池塘水 暴发性 疾病防治 污染问题 养殖业者 多糖 渔药 合理放养 养殖对象
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部