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血卟啉单甲醚介导的声动力疗法对菌斑细菌生物膜作用效果的研究 被引量:1
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作者 闫春阳 王碧琳 +3 位作者 庄德舒 张祎 魏子征 毕良佳 《口腔医学》 CAS 2020年第11期976-981,共6页
目的探讨由超声(ultrasound,US)联合声敏剂血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)产生的声动力疗法(sonodynamic therapy,SDT)对菌斑细菌生物膜活性、膜结构的影响。方法采集牙周炎患者牙菌斑细菌样本,在37℃、厌氧(85%N... 目的探讨由超声(ultrasound,US)联合声敏剂血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)产生的声动力疗法(sonodynamic therapy,SDT)对菌斑细菌生物膜活性、膜结构的影响。方法采集牙周炎患者牙菌斑细菌样本,在37℃、厌氧(85%N2、10%CO2、5%H2)培养。分组:①HMME最小抑菌浓度:选取浓度为0、10、20、30、40、50、60 mg/L的HMME共分成7组;②SDT疗效:Control组、HMME组(50 mg/L为最小抑菌浓度)、US组、SDT组。检测:牙菌斑中细菌在超声强度为3 W/cm2、时间为5 min时HMME的最小抑菌浓度;在SDT作用下牙菌斑的菌落形成单位(colony⁃forming units,CFU);平板计数法检测细菌的存活率;菌斑细菌生物膜活性及形态结构的变化。结果①当超声作用强度为3 W/cm2、5 min时,作为声敏剂的HMME最小抑菌浓度为50 mg/L,剂量-效应关系显著(P<0.05)。②在细菌生物膜存活率比较中,SDT组远低于其他组(P<0.05)。③相比于Control组菌斑细菌边缘清晰、细菌集聚、管状结构完好的形态,SDT组的菌斑细菌边缘破坏、散在分布、结构不再完整。④SDT组的菌斑细菌生物膜相比于Control组、HMME组、超声(ultrasound,US)组可见死菌占比大。结论SDT对菌斑细菌生物膜有一定的抑菌作用,在抑菌的过程中死菌比例增加,并破坏细菌生物膜的结构,产生杀伤效果。 展开更多
关键词 菌斑细菌生物膜 声动力疗法 血卟啉单甲醚 生物膜结构
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肿痛安胶囊联合西地碘含片治疗糖尿病性牙龈炎的疗效 被引量:1
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作者 闫晗 李志 《实用中西医结合临床》 2023年第1期70-72,79,共4页
目的:探讨肿痛安胶囊联合西地碘含片治疗糖尿病性牙龈炎的疗效及机制。方法:按随机数字表法将2021年1~12月就诊的76例糖尿病性牙龈炎患者分为对照组和研究组,对照组采用西地碘含片治疗,研究组采用肿痛安胶囊+西地碘含片治疗。比较两组... 目的:探讨肿痛安胶囊联合西地碘含片治疗糖尿病性牙龈炎的疗效及机制。方法:按随机数字表法将2021年1~12月就诊的76例糖尿病性牙龈炎患者分为对照组和研究组,对照组采用西地碘含片治疗,研究组采用肿痛安胶囊+西地碘含片治疗。比较两组临床疗效,治疗前后牙周指标[牙龈指数(GI)、菌斑指数(PLI)、龈沟出血指数(SBI)]、龈沟液致炎因子水平[白细胞介素(IL)-1β、IL-6、IL-8、转化生长因子-β1(TGF-β1)]、龈下菌斑细菌含量(梭杆菌、拟杆菌、乳杆菌)以及不良反应发生情况。结果:研究组治疗总有效率(97.37%)较对照组(81.58%)高(P<0.05)。治疗后研究组GI、PLI、SBI评分和龈沟液IL-6、IL-8、IL-1β水平均低于对照组,龈沟液TGF-β1水平高于对照组(P<0.05)。治疗后研究组龈下菌斑中梭杆菌、拟杆菌、乳杆菌数量低于对照组(P<0.05)。研究组不良反应发生率(7.89%)与对照组(5.26%)比较相当(P>0.05)。结论:肿痛安胶囊辅助治疗糖尿病性牙龈炎可提高临床疗效,有助于改善牙周状况,抑制炎症反应,减少牙龈菌斑细菌数量,且不会明显增加不良反应。 展开更多
关键词 糖尿病性牙龈炎 肿痛安胶囊 西地碘含片 菌斑细菌 致炎因子
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Advance in Rice Bacterial Leaf Streak Researches 被引量:4
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作者 张荣胜 陈志谊 刘永锋 《Agricultural Science & Technology》 CAS 2015年第2期298-305,310,共9页
Rice bacterial leaf streak,caused by Xanthomonas oryzae pv.oryzicola,is an important bacterial disease in rice-planting areas in South China and Southeast Asian countries.It occurs every year in local areas.This paper... Rice bacterial leaf streak,caused by Xanthomonas oryzae pv.oryzicola,is an important bacterial disease in rice-planting areas in South China and Southeast Asian countries.It occurs every year in local areas.This paper reviews the research advance in the occurrence and harms of rice bacterial leaf streak in China,classification of Xanthomonas oryzae and infection characteristics,pathogenic factors and pathogenicity of rice bacterial leaf streak and breeding of bacterial leaf streakresistance rice cultivars.Some issues involved in occurrence and control of rice bacterial leaf streak were presented,and the areas on which the future studies might be focused were discussed. 展开更多
关键词 Rice bacterial leaf steak Xanthomonas oryzae Occurrence and harm Control
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Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara 被引量:2
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作者 鄢庆枇 赵敏慧 +2 位作者 王晓露 邹文政 陈昌生 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第2期260-266,共7页
Vibriofluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assess... Vibriofluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of K fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V.fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment. 展开更多
关键词 Vibrio fluvialis Epinephelus awoara skin mucus ADHESION
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Differentiation of xanthomonads causing the bacterial leaf spot of poinsettia in China from the pathotype strain of Xanthomonas axonopodis pv. poinsettiicola 被引量:4
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作者 李斌 谢关林 SWINGS J 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第6期451-453,共3页
In October 2003, a new bacterial disease with symptoms similar to those caused by Xanthomonas axonopodis pv. poinsettiicola was observed on poinsettia leaves at a flower nursery in Zhejiang Province of China. Three Xa... In October 2003, a new bacterial disease with symptoms similar to those caused by Xanthomonas axonopodis pv. poinsettiicola was observed on poinsettia leaves at a flower nursery in Zhejiang Province of China. Three Xanthomonas strains were isolated from infected plants and classified as X. axonopodis. They were differentiated from the pathotype strain LMG849 of X. axonopodis pv. poinsettiicola causing bacterial leaf spot of poinsettia by comparison of pathogenicity, substrate utilization and BOX-PCR genomic fingerprints. 展开更多
关键词 Bacterial leaf spot of poinsettia BOX-PCR Hypersensitive reaction PATHOGENICITY X. axonopodis pv. poinsettiicola
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蒙西医结合治疗口腔疾病
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作者 吴双玉 马.额尔敦 《中国民族民间医药》 2005年第4期229-229,共1页
关键词 蒙西医结合治疗 口腔疾病 牙周病 细菌菌斑 川芎液 维生素C注射液
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