In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile ...In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile explants can be obtained. The results revealed the optimal procedures. In detail, the parental materials were trans- ferred and pretreated in laboratory in February. The stem tips of new branches were collected in middle March, cleaned with eradicator and then rinsed with tap water for 1-2 h. After that, the explants were surface sterilized with 70% alcohol for 30 s, rinsed with sterile water 2-3 times, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water three times and sterilized with 0.1% mercuric chlo- ride for 4 min again. Finally, they were inoculated into medium after being rinsed with sterile water 5-8 times. By this method, the pollution rate of explants can be greatly reduced while their survival rate can be significantly improved.展开更多
Medicinal solid fermentation technology of fungal was unique within the traditional Chinese medicine （TCM） and one of the oldest bio-pharmaceutical technology in the history, for example, the production of medicated...Medicinal solid fermentation technology of fungal was unique within the traditional Chinese medicine （TCM） and one of the oldest bio-pharmaceutical technology in the history, for example, the production of medicated leaven named ＂Shenqu＂, the history of which had continued for more than 1,500 years. These kinds of productions were widly used and had a close relationship with TCM. The development of this technology had through stages of ＂yeast production＂, ＂solid culture＂, ＂solid fermentation＂, and nowadays toward a series engineering of solid fermentation with promising prospects. The author described the development of its history briefly, and the representative products and the characteristics of production process of every stage, respectively. This research focused on taking ＂Trametes robinioplila fermented substance＂, ＂T. robinioplila ＆ A. membranaceus fermented substance＂ and ＂T. wilfordii Hook.f. ＆ G. lucidum fermented substance＂ as examples to introduce R ＆ D ideas and important findings of＂common fermentation＂ and ＂bi-directional fermentation＂ which were recommended as studying hotspots. There were many unique and important functions in the fields of TCM about all kinds of＂fermentation substances＂. It is infered that there may exist some other innovate technologies such as ＂Multi-fungal fermentation＂, ＂Special elements fermentation＂, etc. which are remained to be researched and developed continuely. All kinds of＂fermentation substances＂ are looked forward to show unique and important roles in the fields of TCM.展开更多
基金Supported by the Research Funds of Jiangsu Open University/the City Vocational College of Jiangsu during the 12th Five-Year Plan Period(12SEW-Y-026)~~
文摘In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile explants can be obtained. The results revealed the optimal procedures. In detail, the parental materials were trans- ferred and pretreated in laboratory in February. The stem tips of new branches were collected in middle March, cleaned with eradicator and then rinsed with tap water for 1-2 h. After that, the explants were surface sterilized with 70% alcohol for 30 s, rinsed with sterile water 2-3 times, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water three times and sterilized with 0.1% mercuric chlo- ride for 4 min again. Finally, they were inoculated into medium after being rinsed with sterile water 5-8 times. By this method, the pollution rate of explants can be greatly reduced while their survival rate can be significantly improved.
文摘Medicinal solid fermentation technology of fungal was unique within the traditional Chinese medicine （TCM） and one of the oldest bio-pharmaceutical technology in the history, for example, the production of medicated leaven named ＂Shenqu＂, the history of which had continued for more than 1,500 years. These kinds of productions were widly used and had a close relationship with TCM. The development of this technology had through stages of ＂yeast production＂, ＂solid culture＂, ＂solid fermentation＂, and nowadays toward a series engineering of solid fermentation with promising prospects. The author described the development of its history briefly, and the representative products and the characteristics of production process of every stage, respectively. This research focused on taking ＂Trametes robinioplila fermented substance＂, ＂T. robinioplila ＆ A. membranaceus fermented substance＂ and ＂T. wilfordii Hook.f. ＆ G. lucidum fermented substance＂ as examples to introduce R ＆ D ideas and important findings of＂common fermentation＂ and ＂bi-directional fermentation＂ which were recommended as studying hotspots. There were many unique and important functions in the fields of TCM about all kinds of＂fermentation substances＂. It is infered that there may exist some other innovate technologies such as ＂Multi-fungal fermentation＂, ＂Special elements fermentation＂, etc. which are remained to be researched and developed continuely. All kinds of＂fermentation substances＂ are looked forward to show unique and important roles in the fields of TCM.
