为了筛选合适的草菇液体菌种培养条件,本实验设计三个配方进行草菇液体菌种摇床培养对比试验,筛选出适宜的培养基配方,即马铃薯50 g,葡萄糖20 g,蛋白胨1.5 g,KH2PO4 1 g,MgSO4 0.5 g,维生素B1 10 mg,水1000 mL,pH自然。在此基础上设计...为了筛选合适的草菇液体菌种培养条件,本实验设计三个配方进行草菇液体菌种摇床培养对比试验,筛选出适宜的培养基配方,即马铃薯50 g,葡萄糖20 g,蛋白胨1.5 g,KH2PO4 1 g,MgSO4 0.5 g,维生素B1 10 mg,水1000 mL,pH自然。在此基础上设计摇床转速、维生素B1添加浓度、培养基装瓶量、摇前静置时间等四个因子,进行草菇液体菌种摇床培养的比较试验。结果表明,草菇液体菌种培养最适合的摇床转速是130 r/min;添加的维生素B1的最佳浓度为15 mg/L;规格为250 mL的锥形瓶,培养基的最适装瓶量为80 mL;培养基静置36 h再进行摇床培养效果最好。In order to screen suitable culture conditions of liquid strains for Volvariella volvacea, this experiment designed three formulas for comparative shaking table cultivation of V. volvacea liquid strains. The suitable medium formula was selected, namely 50 g of potato, 20 g of glucose, 1.5 g of peptone, 1 g of KH2PO4, 0.5 g of MgSO4, 10 mg of Vitamin B1, 1000 mL of water, and natural pH. On this basis, four factors were designed, including shaking table speed, vitamin B1 addition concentration, bottling volume of culture medium, and settling time before shaking, to conduct comparative experiments on V. volvacea liquid strain shaking table cultivation. The results showed that the most suitable shaking table speed for the cultivation of V. volvacea liquid strains was 130 r/min;the optimal concentration of added vitamin B1 was 15 mg/L;the optimal filling volume for a 250 mL conical flask and culture medium was 80 mL;the best effect was to let the culture medium stand for 36 hours before shaking it on a shaker.展开更多
采用摇床培养的方法,以菌丝球的生物量为评价指标,通过单因素试验和正交试验,说明Zn、Fe、Mn、Cu 四种矿质元素对茶树菇液体菌丝生长的影响。结果表明:Zn、Fe、Mn、Cu四种矿质元素对茶树菇液体菌种菌丝生长具有促进作用,其中Zn对茶树菇...采用摇床培养的方法,以菌丝球的生物量为评价指标,通过单因素试验和正交试验,说明Zn、Fe、Mn、Cu 四种矿质元素对茶树菇液体菌丝生长的影响。结果表明:Zn、Fe、Mn、Cu四种矿质元素对茶树菇液体菌种菌丝生长具有促进作用,其中Zn对茶树菇菌丝生长的影响最明显,其次为Fe、Mn、Cu。这四种矿质元素可以通过单独添加发挥作用,也可以混合添加共同作用,混合添加比单独添加效果更好,促进茶树菇菌丝生长的最优组合是0.25 g/L ZnSO4·7H2O,0.30 g/L FeSO4·7H2O,0.20 g/L MnSO4·H2O,0.15 g/L CuSO4·5H2O。Using the shaking table cultivation method and using the biomass of mycelium balls as the evaluation index, single factor experiments and orthogonal experiments were conducted to illustrate the effects of four mineral elements, Zn, Fe, Mn, and Cu, on the liquid mycelial growth of Agrocybe aegerita. The results showed that four mineral elements, Zn, Fe, Mn, and Cu, had a promoting effect on the mycelial growth of liquid Agrocybe aegerita strains. Among them, Zn had the most significant effect on the mycelial growth of Agrocybe aegerita, followed by Fe, Mn, and Cu. These four mineral elements can exert their effects by adding them alone or by mixing them together. The mixed addition has a better effect than adding them alone. The optimal combination to promote the mycelial growth of Agrocybe aegerita is 0.25 g/L ZnSO4·7H2O, 0.30 g/L FeSO4·7H2O, 0.20 g/L MnSO4·H2O, and 0.15 g/L CuSO4·5H2O.展开更多
文摘为了筛选合适的草菇液体菌种培养条件,本实验设计三个配方进行草菇液体菌种摇床培养对比试验,筛选出适宜的培养基配方,即马铃薯50 g,葡萄糖20 g,蛋白胨1.5 g,KH2PO4 1 g,MgSO4 0.5 g,维生素B1 10 mg,水1000 mL,pH自然。在此基础上设计摇床转速、维生素B1添加浓度、培养基装瓶量、摇前静置时间等四个因子,进行草菇液体菌种摇床培养的比较试验。结果表明,草菇液体菌种培养最适合的摇床转速是130 r/min;添加的维生素B1的最佳浓度为15 mg/L;规格为250 mL的锥形瓶,培养基的最适装瓶量为80 mL;培养基静置36 h再进行摇床培养效果最好。In order to screen suitable culture conditions of liquid strains for Volvariella volvacea, this experiment designed three formulas for comparative shaking table cultivation of V. volvacea liquid strains. The suitable medium formula was selected, namely 50 g of potato, 20 g of glucose, 1.5 g of peptone, 1 g of KH2PO4, 0.5 g of MgSO4, 10 mg of Vitamin B1, 1000 mL of water, and natural pH. On this basis, four factors were designed, including shaking table speed, vitamin B1 addition concentration, bottling volume of culture medium, and settling time before shaking, to conduct comparative experiments on V. volvacea liquid strain shaking table cultivation. The results showed that the most suitable shaking table speed for the cultivation of V. volvacea liquid strains was 130 r/min;the optimal concentration of added vitamin B1 was 15 mg/L;the optimal filling volume for a 250 mL conical flask and culture medium was 80 mL;the best effect was to let the culture medium stand for 36 hours before shaking it on a shaker.
文摘采用摇床培养的方法,以菌丝球的生物量为评价指标,通过单因素试验和正交试验,说明Zn、Fe、Mn、Cu 四种矿质元素对茶树菇液体菌丝生长的影响。结果表明:Zn、Fe、Mn、Cu四种矿质元素对茶树菇液体菌种菌丝生长具有促进作用,其中Zn对茶树菇菌丝生长的影响最明显,其次为Fe、Mn、Cu。这四种矿质元素可以通过单独添加发挥作用,也可以混合添加共同作用,混合添加比单独添加效果更好,促进茶树菇菌丝生长的最优组合是0.25 g/L ZnSO4·7H2O,0.30 g/L FeSO4·7H2O,0.20 g/L MnSO4·H2O,0.15 g/L CuSO4·5H2O。Using the shaking table cultivation method and using the biomass of mycelium balls as the evaluation index, single factor experiments and orthogonal experiments were conducted to illustrate the effects of four mineral elements, Zn, Fe, Mn, and Cu, on the liquid mycelial growth of Agrocybe aegerita. The results showed that four mineral elements, Zn, Fe, Mn, and Cu, had a promoting effect on the mycelial growth of liquid Agrocybe aegerita strains. Among them, Zn had the most significant effect on the mycelial growth of Agrocybe aegerita, followed by Fe, Mn, and Cu. These four mineral elements can exert their effects by adding them alone or by mixing them together. The mixed addition has a better effect than adding them alone. The optimal combination to promote the mycelial growth of Agrocybe aegerita is 0.25 g/L ZnSO4·7H2O, 0.30 g/L FeSO4·7H2O, 0.20 g/L MnSO4·H2O, and 0.15 g/L CuSO4·5H2O.
