不同菌苗制备变形链球菌C型抗血清的比较

目的:比较不同菌苗制备变形链球菌C型抗血清的效果。方法:用变形链球菌血清C型标准株ATCC25175制备成活菌苗和死菌苗,以活菌苗、死菌苗及联合菌苗(活菌苗加死菌苗)3种不同菌苗静脉注射免疫家兔,观察兔子的反应和死亡情况,监测抗体的产生。结果:活菌苗和联合菌苗均在免疫注射的第27～30天出现血清抗体(效价为1∶32),但活菌苗注射后致死率高。结论:联合菌苗是一种较为安全、高效的免疫方案。

白鲢暴发病菌苗的制备及免疫效果分析

在３２℃下对嗜水气单胞菌Ｈ２０株作振荡培养，１％福尔马林、６０℃灭活１ｈｒ．，制备白鲢暴发病菌苗。用该菌苗免疫白鲢，１８～２０℃水温下饲养１０天后，白鲢血清中凝集抗体效价达１：１２８左右，未免疫对照组凝集抗体效价约为１：８。免疫鱼的抗感染保护力达８０％。

辣椒农杆菌介导转化体系的建立及外源基因的转化

辣椒农杆菌介导转化体系主要包括无菌苗制备、菌株培养、农杆菌介导转化、芽诱导、芽伸长、生根及移栽等 6个步骤。芽诱导培养基以MS +4.0～ 6 .0mg/L 6 BA +0 .5mg/LNAA +2 .0mg/LAgNO3 较好 ,诱芽分化率因品种不同而异 ,高的可达 70 %以上 ,子叶的分化率明显高于下胚轴 ;诱芽伸长培养基以MS +3.0mg/L 6 BA +0 .3mg/LNAA +1.0mg/LGA3 较好 ,伸长率达 4 0 % ;生根培养基以 1/2MS +0 .3mg/LNAA(或 0 .5mg/LIAA)较好 ,平均生根率达 6 5 %。经过对 3个品种的抗虫基因转化研究 。

Preparation of Rice Seedling-raising Substrate with Spent Mushroom Substrate of Pleurotus eryngii

This study aimed to explore the effect of fermented spent mushroom substrate(SMS) of Pleurotus eryngii as a basic material on rice seedling-raising substrate. The physical and chemical indices of the SMS indicated that the fermented SMS was fully composted and was very suitable for preparing rice seedling-raising substrate. The fermented SMS effectively regulated the bulk density, total porosity, aeration porosity and water-holding porosity of rice seedling-raising substrate. With the increased addition amount of fermented SMS, the bulk density of rice seedling-raising substrate was decreased, but the total porosity, aeration porosity and water-holding porosity were increased. Compared with those in the substrates of 100% soil and 100% SMS,the height, chlorophyll content, 100-shoot dry weight, 100-root dry weight, root activity, nitrogen content, phosphorus content and potassium content of rice seedlings in the substrate composed of spent mushroom substrate of P. eryngii and soil were higher.The quality of rice seedlings in the substrate composed of 20% soil and 80% SMS was best, followed by that in the substrate composed of 30% soil and 70% SMS.