The research explored the effects of carbon, nitrogen and inorganic salt nutritional factors on Lactarius deliciosus hyphae as per solid culture. The optimal recipe of culture medium was selected by observing hypha de...The research explored the effects of carbon, nitrogen and inorganic salt nutritional factors on Lactarius deliciosus hyphae as per solid culture. The optimal recipe of culture medium was selected by observing hypha density and computing the growth index, which is the most suitable for hypha growth. The results indicated that glucose and peptone were optimal carbon and nitrogen for Lactarius deliciosus;MgSO4, CuSO4 and VB2 promoted hypha growth; FeSO4 and CoCl2 had inhibition effects. The orthogonal test concluded the optimal recipe contained glucose of 20 g/L, peptone of 1 g/L, KH2PO4 of 0.3 g/L, MgSO4 of 0.3 g/L, VB2 of 2 mg/L, and agar of 20 g/L, with original pH value adopted. After optimization, growth index of hyphae cultured at 28 ℃ was 4 055.9.展开更多
基金Supported by the Doctoral Scientific Research Foundation of Jiangxi Agricultural University(09004659)~~
文摘The research explored the effects of carbon, nitrogen and inorganic salt nutritional factors on Lactarius deliciosus hyphae as per solid culture. The optimal recipe of culture medium was selected by observing hypha density and computing the growth index, which is the most suitable for hypha growth. The results indicated that glucose and peptone were optimal carbon and nitrogen for Lactarius deliciosus;MgSO4, CuSO4 and VB2 promoted hypha growth; FeSO4 and CoCl2 had inhibition effects. The orthogonal test concluded the optimal recipe contained glucose of 20 g/L, peptone of 1 g/L, KH2PO4 of 0.3 g/L, MgSO4 of 0.3 g/L, VB2 of 2 mg/L, and agar of 20 g/L, with original pH value adopted. After optimization, growth index of hyphae cultured at 28 ℃ was 4 055.9.
