[Objective] The aim was to study the effects of different acoustic frequency of insect sound （IS） and insect-music mixed sound （MS） treatment on the growth of 6 kinds of vegetables.[Method] The insect acoustic gro...[Objective] The aim was to study the effects of different acoustic frequency of insect sound （IS） and insect-music mixed sound （MS） treatment on the growth of 6 kinds of vegetables.[Method] The insect acoustic group,insect-music mixed group and normal control group were designed to determine the related indices of 6 kinds of vegetables in unified management regularly.[Result] After different acoustic frequency treatment,besides soybean plant height,dry root weight of cabbage and greengrocery,there was significant difference of plant height,fresh weight and dry weight between the other vegetables and control.Under acoustic frequency treatment,the growth situations of six kinds of vegetables were better than control.In the growth period,acoustic frequency treatment could promote height growth （except soybean） and edible part yield.[Conclusion] Both of IS and MS could promote the plant growth,but their effects had difference.展开更多
Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in...Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. The transgenic plants, identified by resistant selection, polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) analyses, and green fluorescence examination, showed growth retardation, flowering delay, and dwarf stature. The fresh weight and dry weight in transgenic lines were about 21% and 29% lower than those in wild type (WT), respectively, and the fresh to dry weight ratios were higher than that of WT. Quantitative measurements demonstrated that the lignin content in transgenic lines decreased by 10%-20%, and histochemical staining results also showed reduced lignification in transgenic lines. Quantitative real-time PCR analysis indicated that the transcript levels of lignin biosynthetic genes in transgenic lines were markedly decreased and were consistent with the reduced lignification. These results suggest that the reduced biomass accumulation and lignification in the AtGA2ox8 over-expression rapeseed might be due to altered lignin biosynthetic gene expression.展开更多
基金Supported by Important Project of Science and Technology of Zhejiang Province(2008C12056)~~
文摘[Objective] The aim was to study the effects of different acoustic frequency of insect sound （IS） and insect-music mixed sound （MS） treatment on the growth of 6 kinds of vegetables.[Method] The insect acoustic group,insect-music mixed group and normal control group were designed to determine the related indices of 6 kinds of vegetables in unified management regularly.[Result] After different acoustic frequency treatment,besides soybean plant height,dry root weight of cabbage and greengrocery,there was significant difference of plant height,fresh weight and dry weight between the other vegetables and control.Under acoustic frequency treatment,the growth situations of six kinds of vegetables were better than control.In the growth period,acoustic frequency treatment could promote height growth （except soybean） and edible part yield.[Conclusion] Both of IS and MS could promote the plant growth,but their effects had difference.
基金Project supported by the National High-Tech R & D Program (863) of China (No. 2007AA10Z127)the National Natural Science Foundation of China (No. 30800080)
文摘Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. The transgenic plants, identified by resistant selection, polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) analyses, and green fluorescence examination, showed growth retardation, flowering delay, and dwarf stature. The fresh weight and dry weight in transgenic lines were about 21% and 29% lower than those in wild type (WT), respectively, and the fresh to dry weight ratios were higher than that of WT. Quantitative measurements demonstrated that the lignin content in transgenic lines decreased by 10%-20%, and histochemical staining results also showed reduced lignification in transgenic lines. Quantitative real-time PCR analysis indicated that the transcript levels of lignin biosynthetic genes in transgenic lines were markedly decreased and were consistent with the reduced lignification. These results suggest that the reduced biomass accumulation and lignification in the AtGA2ox8 over-expression rapeseed might be due to altered lignin biosynthetic gene expression.
