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菠萝皮蛋白酶酶解草鱼鱼鳞提取胶原蛋白的工艺优化 被引量:5
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作者 陈伟杰 耿丽晶 +2 位作者 王馨云 陈博 周围 《饲料研究》 CAS 2016年第13期1-7,共7页
鱼鳞是鱼真皮层的胶原质生成的骨质,含有丰富的营养物质,具有补钙、美容、促消化和预防心血管疾病等保健功效,但生活中却经常被当作是废弃物被扔掉。试验用菠萝皮中提取的蛋白酶来酶解草鱼鱼鳞蛋白,运用响应面优化分析法,以水解度/%作... 鱼鳞是鱼真皮层的胶原质生成的骨质,含有丰富的营养物质,具有补钙、美容、促消化和预防心血管疾病等保健功效,但生活中却经常被当作是废弃物被扔掉。试验用菠萝皮中提取的蛋白酶来酶解草鱼鱼鳞蛋白,运用响应面优化分析法,以水解度/%作为响应值,分析底物质量分数(S)/%、酶解时间/h、酶解温度/℃和加酶量(E/S)/%这4个因素对菠萝皮蛋白酶酶解草鱼鱼鳞蛋白效果的影响,试验获得最佳酶解条件为5%的鱼鳞中加入9%的菠萝皮蛋白酶68℃酶解2.5 h,获得水解度最高为(1.327%±0.040%),该条件具有很好的生产应用前景。 展开更多
关键词 菠萝皮蛋白酶 水解蛋白 响应面法 鱼鳞蛋白
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Incorporation of Bromelain into Dermatological Bases: Accelerated Stability Studies 被引量:1
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作者 Iara Rocha Antunes Pereira Bresolin Igor Tadeu Lazzarotto Bresohn +1 位作者 Priscila Gava Mazzola Elias Basile Tambourgi 《Journal of Chemistry and Chemical Engineering》 2014年第3期270-277,共8页
This work aimed the incorporation of the enzyme bromelain from pineapple peel into dermatological bases for potential therapeutic application. The enzyme was incorporated into Lanette cream and lotion, as well as Carb... This work aimed the incorporation of the enzyme bromelain from pineapple peel into dermatological bases for potential therapeutic application. The enzyme was incorporated into Lanette cream and lotion, as well as Carbopol gel and Chemyunion cream and lotion, at a concentration of 0.5% (w/w). These bases were subjected to centrifugation test and accelerated stability test during 90 days at 25 ℃(with and without sunlight), 37 ℃ and 4 ℃, in order to evaluate bromelain stability in dermatological bases. Total protein content and enzymatic activity were measured. The bases were stable as its organoleptic characteristics (appearance, color, smell and sensitivity to touch) only when kept at 4 ℃ with activity remaining 95.5%, 84,9%. 77.7%, 73.8% and 72.3%, alter 90 days of testing in Carbopol gel, Lanette and Chemyunion cream, and Lanette and Chemyunion lotion, respectively. Based on the results, it was possible to incorporate bromelain into dermatological bases, and the activity was well preserved when these bases were kept in refrigerator at 4 ℃. 展开更多
关键词 Emulsions STABILITY BROMELAIN therapeutic applications enzymatic activity.
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