[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and H...[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. [Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. [Conclusion] This study provided theoretical references for breeding drought-tolerant wheat.展开更多
Effects of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on the germination and metabolism of reactive oxygen species were surveyed in wheat (Triticum aestivum L.) seeds. Germination of wheat seeds and even t...Effects of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on the germination and metabolism of reactive oxygen species were surveyed in wheat (Triticum aestivum L.) seeds. Germination of wheat seeds and even the elongation of radicle and plumule were dramatically promoted by SNP treatments during the germination under osmotic stress. Meanwhile, activities of amylase and EP were enhanced, thus leading to the degradation of storage reserve in seeds. After osmotic stress was removed, higher viability of wheat seeds was also maintained. In addition, the activities of CAT, APX and the content of proline were increased by SNP treatment simultaneously, but activities of LOX were inhibited, and both of which were beneficial for improving the antioxidant capacity during the germination of wheat seeds under osmotic stress. It was also shown that the increase of the activity of amylase induced by SNP in embryoless half-seeds of wheat in the beginning period of germination (6 h) might be indirectly related to GA(3).展开更多
Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme fo...Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme for Phytophthora to foot rot resistance in the cultivated species, P. nigrum. This project was carried out to reveal the optimum time to collect pollen of P. colubrinum for artificial pollination. Through this study, anther dehiscence was proved occurred around 9 am. Based on the result obtained from study on the time of anther dehiscence, the pollen was classified into five stages for testing on the viability, i.e., stage 1, before anther dehiscence, i.e., around 7 am （pollen obtained by crushing on the anther）; stage 2： immediately after anther dehiscence, i.e., around 9 am; stage 3： two hours after anther dehiscence, i.e., around 11 am; stage 4： four hours after anther dehiscence, i.e., around 1 pm; and stage 5： six hours after anther dehiscence, i.e., around 3 pm. Data obtained from pollen germination and pollen tube growth study showed that pollen of P. colubrinum was the most viable at two hours after anther dehiscence and onward. It was also proved that the viability can be maintained up to at least eight hours. Pollen obtained before anther dehiscence by crushing on the anther was found less viable with lower percentage of pollen germination and retarded pollen tube growth. In other words, the experiment enlightened that artificial pollination between P. nigrum L. and P. colubrinum Link was ideally to be carded out approximately starting from 11 am until 5 pm, considering the viability of pollen ofP. colubrinum.展开更多
The aim of this work is to identify the effect of lead on germinal parameters and the antioxidant enzyme activities （lipase, peroxidase and catalase） in durum wheat Triticum durum Desf. cv （waha, vitron and gta） e...The aim of this work is to identify the effect of lead on germinal parameters and the antioxidant enzyme activities （lipase, peroxidase and catalase） in durum wheat Triticum durum Desf. cv （waha, vitron and gta） exposed to the concentrations of 0, 0.15, 0.25 and 0.3 g/L of Pb （NO3）2 during germination process. The obtained results showed that lead reduced the germination, root and aerial biomass. The concentration of 0.3 g/L inhibited completely the germination of the three varieties. It also slowed lipase activity, the degradation of lipids of the seed＇s reserves and disrupted the metabolism of peroxidase and catalase. Concerning the behavior of the three varieties studied, it appears that the Vitron is the best predisposed variety to stand against lead stress by its strong antioxidant defense system.展开更多
Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ...Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize （Zea rnays L.） inbred lines, HuangC （chilling-tolerant） and Mo17 （chilling-sensitive）. While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time （MGT）, and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde （MDA） content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase （POD） activity, and catalase （CAT） activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. Priming with 0.50% chitosan for about 60-64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.展开更多
基金Supported by Natural Science Fund of Education Department of Anhui Province(KJ2012B188)General Science and Research Program of Suzhou College(2011yyb13)~~
文摘[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. [Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. [Conclusion] This study provided theoretical references for breeding drought-tolerant wheat.
文摘Effects of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on the germination and metabolism of reactive oxygen species were surveyed in wheat (Triticum aestivum L.) seeds. Germination of wheat seeds and even the elongation of radicle and plumule were dramatically promoted by SNP treatments during the germination under osmotic stress. Meanwhile, activities of amylase and EP were enhanced, thus leading to the degradation of storage reserve in seeds. After osmotic stress was removed, higher viability of wheat seeds was also maintained. In addition, the activities of CAT, APX and the content of proline were increased by SNP treatment simultaneously, but activities of LOX were inhibited, and both of which were beneficial for improving the antioxidant capacity during the germination of wheat seeds under osmotic stress. It was also shown that the increase of the activity of amylase induced by SNP in embryoless half-seeds of wheat in the beginning period of germination (6 h) might be indirectly related to GA(3).
文摘Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme for Phytophthora to foot rot resistance in the cultivated species, P. nigrum. This project was carried out to reveal the optimum time to collect pollen of P. colubrinum for artificial pollination. Through this study, anther dehiscence was proved occurred around 9 am. Based on the result obtained from study on the time of anther dehiscence, the pollen was classified into five stages for testing on the viability, i.e., stage 1, before anther dehiscence, i.e., around 7 am （pollen obtained by crushing on the anther）; stage 2： immediately after anther dehiscence, i.e., around 9 am; stage 3： two hours after anther dehiscence, i.e., around 11 am; stage 4： four hours after anther dehiscence, i.e., around 1 pm; and stage 5： six hours after anther dehiscence, i.e., around 3 pm. Data obtained from pollen germination and pollen tube growth study showed that pollen of P. colubrinum was the most viable at two hours after anther dehiscence and onward. It was also proved that the viability can be maintained up to at least eight hours. Pollen obtained before anther dehiscence by crushing on the anther was found less viable with lower percentage of pollen germination and retarded pollen tube growth. In other words, the experiment enlightened that artificial pollination between P. nigrum L. and P. colubrinum Link was ideally to be carded out approximately starting from 11 am until 5 pm, considering the viability of pollen ofP. colubrinum.
文摘The aim of this work is to identify the effect of lead on germinal parameters and the antioxidant enzyme activities （lipase, peroxidase and catalase） in durum wheat Triticum durum Desf. cv （waha, vitron and gta） exposed to the concentrations of 0, 0.15, 0.25 and 0.3 g/L of Pb （NO3）2 during germination process. The obtained results showed that lead reduced the germination, root and aerial biomass. The concentration of 0.3 g/L inhibited completely the germination of the three varieties. It also slowed lipase activity, the degradation of lipids of the seed＇s reserves and disrupted the metabolism of peroxidase and catalase. Concerning the behavior of the three varieties studied, it appears that the Vitron is the best predisposed variety to stand against lead stress by its strong antioxidant defense system.
基金Project supported by the Major Science and Technology Special Project (priority subject) of Zhejiang Province (No. 2008C12005-1)the Key Project of Education Department of Zhejiang Province (No. 20070147), China
文摘Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize （Zea rnays L.） inbred lines, HuangC （chilling-tolerant） and Mo17 （chilling-sensitive）. While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time （MGT）, and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde （MDA） content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase （POD） activity, and catalase （CAT） activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. Priming with 0.50% chitosan for about 60-64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.
