It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grow...It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active.展开更多
The opposition between the terms carcasse （carcass）, conceptualized by Auguste Perret, and ossature （frame）, proposed as an alternative by Le Corbusier, gives rise to the exploration of the capital contribution of...The opposition between the terms carcasse （carcass）, conceptualized by Auguste Perret, and ossature （frame）, proposed as an alternative by Le Corbusier, gives rise to the exploration of the capital contribution of the ＂Dom-ino＂ prototype as the basic and in escapable condition for an aesthetic operation. Some issues addressed are： the importance of the question of the structure--which remains implicit in Toward an Architecture--as key to a quest for the specificity of architecture; Le Corbusier＇s troublesome relationship with Perret and the debates between them, which convey two different ways of understanding the potential contributions of concrete to the redefinition of architectural vocabulary; the ＂Dom-ino＂ system considered as a new structural type in the sense ascribed to this category by Violletle Duc; the topic of the abri souverain （sovereign shelter） fit for all programs, which triggered typological invention; the ways in which Le Corbusier plays with Gottfried Semper＇s Urformen and, finally, how this new structural type anchors Le Corbusier＇s radical redefinition of the elements of the discipline, the making of a new grammar.展开更多
文摘It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active.
文摘The opposition between the terms carcasse （carcass）, conceptualized by Auguste Perret, and ossature （frame）, proposed as an alternative by Le Corbusier, gives rise to the exploration of the capital contribution of the ＂Dom-ino＂ prototype as the basic and in escapable condition for an aesthetic operation. Some issues addressed are： the importance of the question of the structure--which remains implicit in Toward an Architecture--as key to a quest for the specificity of architecture; Le Corbusier＇s troublesome relationship with Perret and the debates between them, which convey two different ways of understanding the potential contributions of concrete to the redefinition of architectural vocabulary; the ＂Dom-ino＂ system considered as a new structural type in the sense ascribed to this category by Violletle Duc; the topic of the abri souverain （sovereign shelter） fit for all programs, which triggered typological invention; the ways in which Le Corbusier plays with Gottfried Semper＇s Urformen and, finally, how this new structural type anchors Le Corbusier＇s radical redefinition of the elements of the discipline, the making of a new grammar.
