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南瓜类胡萝卜素高通量提取检测体系建立及应用
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作者 宋慧 安学君 +1 位作者 黄芸萍 张香琴 《农学学报》 2024年第3期21-27,共7页
为了建立南瓜类胡萝卜素高通量提取检测体系,以南瓜果肉为试材,通过单因素试验和L27(313)正交试验,确定和优化南瓜类胡萝卜素提取的最佳提取液、提取温度和料液比。结果表明,乙醇是提取南瓜类胡萝卜素的最佳提取液,在55℃条件下,以料液... 为了建立南瓜类胡萝卜素高通量提取检测体系,以南瓜果肉为试材,通过单因素试验和L27(313)正交试验,确定和优化南瓜类胡萝卜素提取的最佳提取液、提取温度和料液比。结果表明,乙醇是提取南瓜类胡萝卜素的最佳提取液,在55℃条件下,以料液比1:28,浸提90 min,类胡萝卜素的得率显著高[(7.56±0.14) mg/100 g]。在南瓜类胡萝卜素的提取条件中,提取温度对提取效果影响最大,料液比×提取时间和提取时间×温度的互作次之。利用高通量提取检测体系比较分析23份不同类型南瓜材料类胡萝卜素含量,发现印度南瓜‘贝栗4号’和中国南瓜×印度南瓜远缘杂种自交系20F8的类胡萝卜素含量分别为(31.40±0.35) mg/100g和(24.72±0.72) mg/100 g,显著高于其他供试南瓜材料,具有显著的营养利用价值。试验获得的南瓜类葫芦卜素高效检测体系,省事省力,结果准确,可提高南瓜营养品质育种效率。 展开更多
关键词 南瓜 类胡萝卜素 高通量测定 远缘杂交 营养品质育种
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不同类型水稻品种的稻米蛋白质含量及其氨基酸组成的研究 被引量:5
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作者 黄英金 刘宜柏 《江西农业学报》 CAS 1989年第2期25-31,共7页
本研究系统分析了目前生产上主栽的早籼、晚籼、杂交籼稻、晚粳及特种稻等不同类型品种(组合)的稻米蛋白质含量及其氨基酸组成.结果表明:目前主栽品种中,稻米蛋白质含量的差异很大;稻米蛋白质缺乏赖、苏、色、含硫氨基酸,尤以赖氨酸是... 本研究系统分析了目前生产上主栽的早籼、晚籼、杂交籼稻、晚粳及特种稻等不同类型品种(组合)的稻米蛋白质含量及其氨基酸组成.结果表明:目前主栽品种中,稻米蛋白质含量的差异很大;稻米蛋白质缺乏赖、苏、色、含硫氨基酸,尤以赖氨酸是所有供试品种(组合)的第一限制性氨基酸;各种氨基酸含量的高低顺序在不同品种(组合)中基本相似,都以谷、亮氨酸较高,色、蛋氨酸较低;各种氨基酸的含量都随稻米蛋白质含量的增减而不同程度地相应增减,但其中几种限制性氨基酸的增减程度最小.本研究中所有高蛋白品种,限制性氨基酸的相对含量反而较低.本文提出在保持较高蛋白质含量的基础上提高稻米蛋白质的质量是一个很值得重视的问题,并对提高几种限制性氨基酸相对含量的育种途径进行了讨论. 展开更多
关键词 水稻 蛋白质 限制性氨基酸 营养品质育种
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Genetic Differences and Selection of Major Nutritional Quality Characters in Millet
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作者 张爱霞 刘晓东 +2 位作者 王桂荣 李珊珊 王慧军 《Agricultural Science & Technology》 CAS 2013年第1期35-39,共5页
[Objective] The aim was to enhance specificity and efficiency of quality breeding of millet. [Method] Difference analysis, correlation analysis, principal compo- nent analysis, and path analysis were conducted on nutr... [Objective] The aim was to enhance specificity and efficiency of quality breeding of millet. [Method] Difference analysis, correlation analysis, principal compo- nent analysis, and path analysis were conducted on nutritional quality characters of 29 millet varieties. [Result] Variance coefficients of nutritional quality characters among different varieties were in descending order of crude protein 〉 crude fiber 〉 fat 〉 water 〉 total carbohydrate. The results of correlation analysis suggested that crude protein exhibited extremely significant negative correlation with total carbohydrate (correlation coefficient of -0.822 4) and significantly negative correlation with fat (correlation coefficient of -0.380 7); the rest characters showed no significant correla- tion. In addition, according to PCA and path analysis, four characters, including crude protein, fat, total carbohydrate and crude fiber, determined 97.35% of variance of nutritional quality and could be taken as references for selection of breeding ma- terials, with a determination coefficient of 1.000 0. Fat and carbohydrate were typical nutritional quality characters of breeding materials, which should be selected in low generation. However, crude protein and crude fiber should be selected for millet in high generation. [Conclusion] The research provides references for breeding of new millet varieties with high nutritional quality. 展开更多
关键词 MILLET Nutritional quality characters BREEDING
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Breeding and Evaluation of New Iron Yam (Dioscorea opposita Thunb) Varieties
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作者 Sufang DUAN Jianjun LI +3 位作者 Meiling REN Jun WANG Guolun JIA Shuwu SUN 《Agricultural Science & Technology》 CAS 2015年第10期2121-2125,共5页
[Objective] This study was conducted to develop some new iron yam (Dioscorea opposita) varieties with excellent cooking, eating and nutritional qualities. [Method] The fresh weight per plant, drying rate, dry weight... [Objective] This study was conducted to develop some new iron yam (Dioscorea opposita) varieties with excellent cooking, eating and nutritional qualities. [Method] The fresh weight per plant, drying rate, dry weight per plant, yield, allan- toin content, water soluble extract content and disease resistance of seven new iron yam cultivars (No.1, No.2, No.4, No.6, No.8, No.9 and No.10) obtained through spaceflight mutation breeding were measured and compared with control (No.11). Then, the nutrition quality of two elite cultivars No. 6 and No.10 was determined by comparing with the control. [Result] The eight iron yam cultivars ranked in descend- ing order of their fresh weight per plant as follows: No.6(0.255 kg)〉No.10(0.254 kg)〉 No.4(0.242 kg)〉No.9(0.237 kg)〉No.1(0.233 kg)〉No.11(0.206 kg)〉No.2(0.191 kg)〉No.8 (0.157 kg). There was no significant difference in fresh weight per plant between No.6 and No.10, but extremely significant difference between No.6 and the control, No.10 and the control. The eight iron yam cultivars ranked in descending order of their drying rate as follows: No.2 (32.641%)〉No.10 (32.230%)〉No.9 (28.223%)〉No.6 (25.174%)〉No.8(25.122%)〉No.11 (25.043%)〉No.1 (24.291%)〉No.4(20.234%). The dry- ing rate of both No.6 and No.10 was higher than that of the control. The eight iron yam cultivars ranked in descending order of their dry weight per plant as follows: No.10(0.082 kg)〉No.9(0.067 kg)〉No.6(0.064 kg)〉No.2(0.062 kg)〉No.1(0.056 kg)〉No.11 (0.052 kg)〉No.4 (0.049 kg)〉No.8 (0.039 kg). The dry weight per plant of both No.6 and No.10 is higher than that of the control. The eight iron yam cultivars ranked in descending order of their allantoin content as follows No.6 (0.484%)〉No.4 (0.467%)〉 No.10 (0.399%)〉No.ll (0.386%)〉No.9 (0.378%)〉No.8 (0.350%)〉No.2 (0.342)〉No.1 (0.325%). The allantoin content of No.6 was significantly higher than that of No.10 and the control. There was significant difference in allantoin content between No.6 and No.10, but no significant difference between No 10 and the control. The eight iron yam cultivars ranked in descending order of their extract content as follows No. 6(20.49%)〉No.2(16.01%)〉No.4(15.54%) 〉No. 10(15.35%)〉No. 1 (14.48%)〉No. 11 (14.10%) 〉No.9(13.88%)〉No.8(11.87%). The extract content of No.6 exhibited extremely sig- nificant difference with that of No.10 and the control, and the extract content of No. 10 was also significantly different from that of the control. The taste of No.10 was dry, soft, floury, sweet and fragrant, better than that of the other seven cultivars. No.6 had the strongest resistance to Gloeosporium pestis and Cylindrosporium dioscoreae. No.10 had a middle resistance to G. pestis and a strong resistance to C. dioscoreae. No.6 and No.10 had higher contents of starch, reducing sugar, pro- tein and ash, lower water content and better nutrition quality than the control. [Conclusion] No.10 can be popularized as a new variety of edible iron yam, and No.6 as a new variety of medicinal iron yam. 展开更多
关键词 Iron yam New cultivar BREEDING Production Medicinal ingredients Nu- trition quality
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