This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven tr...This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-MS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEM–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent DOM component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and Mg were significantly higher than Ca,Fe,Mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.展开更多
This article is one of the serial studies oll the characteristics of the molecular structure for dystrophin gene in Chinese. By using the entire dystrophin cDNA (14 kb) as a probe- the number and RFLPs of Bgl Ⅱ exon-...This article is one of the serial studies oll the characteristics of the molecular structure for dystrophin gene in Chinese. By using the entire dystrophin cDNA (14 kb) as a probe- the number and RFLPs of Bgl Ⅱ exon-containing fragments of the dystrophin gene were analysed. Four new Bgl Ⅱ fragments were found, two of them (3.7 and 6.2 kb) detected by comparing the hybridization patterns with cDNA1-2a. 1a and 2a, one (9.3 kb) from the hybridization pattern with cDNA 9 by lengthening migrating distance of DNA fragments in electrophoresis. and another one (4.0 kb) by comparing the patterns with cDNA 11-14,11a- 11b’ 11c-12a and 14. The results indicated that the number of Bgl Ⅱ exon-containing fragments should be 59 rather than 55 reported previously, which laid the foundation of the Bgl Ⅱ partial restriction map for dystrophin gene. Three of the four RFLPs found in Caucacian appear in the hybridization patterns of three subclones, i.e.cDNA 2b-3. cDNA 4-5, and cDNA 5b-7.’ The values of expected heterozygote frequency (EHF) were 0.33, 0.33and 0.40 and the observed heterozygote frequency (OHF)were 0.40. 0.40 and 0.48 respectively. Meanwhile, two new rare allelic fragments (15 kb) were found in RFLPs from Bgl Ⅱ/2b-3 and Bgl Ⅱ/4-5a patterns respectively. These Bgl Ⅱ RFLPs and four XbaI RFLPs documented in our laboratory, have been used to detect the carrier in 7 DMDfamilies and 1 BMD family. Of the 69 individuals from the 8 families- 11 females were diagnosed as the carriers with DMD mutation, 4 females as the doubtful carriers, 12 females were defined as normal genotype and 2 females as probably normal. The results suggest that the carrier testing method based on dosage intensity analysis and genotype analysis by using dystrophin cDNA as a probe will be more sensitive and accurate.展开更多
Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention...Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention. Methods： A total of 43 patients with severe craniocerebral injury admitted to our department from January 2008 to December 2012 received PEG followed by nutritional therapy. There were other 82 patients who were prescribed nasal-feeding nutrition. Nutrition status was evaluated by comparing serum albumin levels, and the incidence of pulmonary infection 1 week before and 2 weeks after operation was identified and compared. Results： Both PEG and nasal-feeding nutrition therapies have significantly elevated serum albumin levels （P〈0.05）. Serum albumin levels before and after nutritional therapies showed no significant difference between the two groups （P〉0.05）. The incidence of pulmonary infection in PEG group was significantly decreased compared with that in nasal-feeding nutrition group （P〈0.05）. Conclusion： PEG is an effective method for severe craniocerebral injury patients. It can not only provide enteral nutrition but also prevent pulmonary infection induced by esophageal reflux.展开更多
基金financially supported by the Key Construction Program of the National 985 Project,Tianjin University,Chinathe National Key R and D Program of China (2016YFA0601000)
文摘This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-MS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEM–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent DOM component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and Mg were significantly higher than Ca,Fe,Mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.
文摘This article is one of the serial studies oll the characteristics of the molecular structure for dystrophin gene in Chinese. By using the entire dystrophin cDNA (14 kb) as a probe- the number and RFLPs of Bgl Ⅱ exon-containing fragments of the dystrophin gene were analysed. Four new Bgl Ⅱ fragments were found, two of them (3.7 and 6.2 kb) detected by comparing the hybridization patterns with cDNA1-2a. 1a and 2a, one (9.3 kb) from the hybridization pattern with cDNA 9 by lengthening migrating distance of DNA fragments in electrophoresis. and another one (4.0 kb) by comparing the patterns with cDNA 11-14,11a- 11b’ 11c-12a and 14. The results indicated that the number of Bgl Ⅱ exon-containing fragments should be 59 rather than 55 reported previously, which laid the foundation of the Bgl Ⅱ partial restriction map for dystrophin gene. Three of the four RFLPs found in Caucacian appear in the hybridization patterns of three subclones, i.e.cDNA 2b-3. cDNA 4-5, and cDNA 5b-7.’ The values of expected heterozygote frequency (EHF) were 0.33, 0.33and 0.40 and the observed heterozygote frequency (OHF)were 0.40. 0.40 and 0.48 respectively. Meanwhile, two new rare allelic fragments (15 kb) were found in RFLPs from Bgl Ⅱ/2b-3 and Bgl Ⅱ/4-5a patterns respectively. These Bgl Ⅱ RFLPs and four XbaI RFLPs documented in our laboratory, have been used to detect the carrier in 7 DMDfamilies and 1 BMD family. Of the 69 individuals from the 8 families- 11 females were diagnosed as the carriers with DMD mutation, 4 females as the doubtful carriers, 12 females were defined as normal genotype and 2 females as probably normal. The results suggest that the carrier testing method based on dosage intensity analysis and genotype analysis by using dystrophin cDNA as a probe will be more sensitive and accurate.
文摘Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention. Methods： A total of 43 patients with severe craniocerebral injury admitted to our department from January 2008 to December 2012 received PEG followed by nutritional therapy. There were other 82 patients who were prescribed nasal-feeding nutrition. Nutrition status was evaluated by comparing serum albumin levels, and the incidence of pulmonary infection 1 week before and 2 weeks after operation was identified and compared. Results： Both PEG and nasal-feeding nutrition therapies have significantly elevated serum albumin levels （P〈0.05）. Serum albumin levels before and after nutritional therapies showed no significant difference between the two groups （P〉0.05）. The incidence of pulmonary infection in PEG group was significantly decreased compared with that in nasal-feeding nutrition group （P〈0.05）. Conclusion： PEG is an effective method for severe craniocerebral injury patients. It can not only provide enteral nutrition but also prevent pulmonary infection induced by esophageal reflux.
