Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements. Proximate composition, amino acid an...Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements. Proximate composition, amino acid and fatty acid profiles, and toxic elements contents of fanned and wild Chanodichthys mongolicus were established and compared. Significantly higher crude protein content while lower moisture content in farmed fish compared to wild fish were observed (P〈0.05). The percentages of total amino acids (TAA), total essential amino acids (TEAA), total non-essential amino acids (TNEAA) and total delicious amino acids (TDAA) in farmed fish were all significantly higher than those in the wild equivalent (P〈0.05). Compared to the FAO/WHO recommended reference values, the ratios of TEAA/ TAA (39.84%o-40.33%) were comparable to 40% and TEAA / TNEAA (66.22%-67.60%) were above 60%. Fatty acid profiles in both farmed and wild C. mongolicus were dominated by monounsaturated fatty acid (MUFA), with farmed fish contained much more MUFA content compared to wild counterpart (P〈0.05). Notably, wild fish exhibited significantly higher levels of total polyunsaturated fatty acid (PUFA) including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) than fanned fish (P〈0.05). The EPA (C20:503) and linoleic acid (C18:206) were the predominant PUFA in wild and farmed C. mongolicus, respectively. Moreover, farmed fish displayed an overall lower toxic element levels (As, Cd, Pb and Hg) in comparison with wild fish, and both were far lower than the established limit standard. In conclusion, our results suggest that the nutritional quality of fanned C. mongolicus was inferior to their wild counterpart with respect to fatty acids nutrition, and therefore further studies should focus on the improving C. mongolicus diet in order to enhance the overall nutritional composition.展开更多
Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds a...Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients (nitrogen, phosphorus, silicon, and sulfur), light intensity and light path, on O. aurita cell growth and biochemical composition (protein, carbohydrate, β-1,3-glucan, lipids, and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of 15-1,3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.展开更多
The macronutrient composition and in vitro protein digestibility of extruded fermented and unfermented extruded soya protein products (low, medium and high moisture), raw and fermented soya meal and soya kernel were...The macronutrient composition and in vitro protein digestibility of extruded fermented and unfermented extruded soya protein products (low, medium and high moisture), raw and fermented soya meal and soya kernel were studied. The protein content (g/100g soya dry weight) ranged from 38.20 to 62.98 with the highest content in the high moisture extruded protein product fermented with 5 mL inoculum of Bacillus natto. Contents of carbohydrates ranged from 14.77 to 29.08 while those of crude fibre, fat and ash were generally low. Fermentation better improved protein digestibility in the raw soya meal and kernel than in the unfermented extruded and extruded fermented products. SDS-PAGE electrophoresis revealed some degradation of the protein sub units of fermented samples.展开更多
AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats un...AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy. METHODS: Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gin. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis. RESULTS: The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6±242.5 vs-233.4±205.9 mg/d, d2, 31.8±238.8 vs-253.4±184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185±931 vs323±201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1β or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-α level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3. CONCLUSION: TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gin supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.展开更多
This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise Cit...This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise City in northern Guangxi to compare plant height, yield, stem-leaf ratio and nutrient composition am-ong Pennisetum purpureum Schumab cv. Purple, P. purpureum Schum cv. Guimin and P. purpureum Rich &#215; P. americana King grass cv. Reyan No.4. The results in-dicated that average annual fresh yield of P. purpureum Schumab cv. Purple reached 141.4 t/hm2, which was 2.3 t/hm^2 higher than that of P. purpureum_Rich×P. americana_King grass cv. Reyan No.4; both average annual fresh yield of these two species was lower than that of P. purpureum Schum cv. Guimin. Stem-leaf ratio of P. purpureum Schumab cv. Purple was the lowest (1.17), which was 17.8% lower compared with P. purpureum Schum cv. Guimin, with significant differences (P〈0.05), indicating that P. purpureum Schumab cv. Purple had more leaves. Crude protein content of P. purpureum_Schumab cv. Purple was slightly lower than that of P. purpureum Rich×P. americana_King grass cv. Reyan No.4, while P. purpureum Schumab cv. Purple was superior to P. purpureum Schum cv. Guimin and P. pur-pureum Rich ×P. americana_King grass cv. Reyan No.4 in other indicators. Specifi-cal y, P. purpureum Schumab cv. Purple contained 26.6% of dry matter, 39.1% of nitrogen-free extract and 2.9% of crude fat.展开更多
The research works were carried out for determination of the washout, types, sorts and level of the development reasons of degradation with the purpose of preparation of the measures of fight against degradation in th...The research works were carried out for determination of the washout, types, sorts and level of the development reasons of degradation with the purpose of preparation of the measures of fight against degradation in the soils in 2005-2009 in the lowland of Kur-Araz. The investigations were fulfilled by using of geographical and stasionar methods. It is revealed that 333.6 thous, ha of the soils of the lowland of Kur-Araz have been exposed to erosion. 97.4 thousand ha of them have been exposed to rainstorm erosion, 127.1 thous, have been exposed to irrigative and 122.1 thous, ha to wind erosion. The most dangerous of them are considered irrigative erosion. Under the influence of erosion process in deposited over the length of furrow, deposits the changes happened in maintenance of humus and nutritions elements. In dependence on slope and water expenditure on 1 ha 2.23-14.86 t of soil are leached. As a result of out wash from 1 ha of soil 55.88-304.59 kg of humus, 4.06-20.80 kg of nitrogen, 4.57-26.55 of phosphorus and 57.40-372.99 of potassium are lost. The soil is exposed to degradation and as a result a process of desertification begins. On the basis of the quantitative an intensity of soil outwash the possible losses of dry matter including humus and main elements of nutrition of plants as a result of erosion, their deposit with the water way, are calculated, too.展开更多
基金Supported by the Central-level Non-profit Scientific Research Institutes Special Funds(No.2014A07XK04)
文摘Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements. Proximate composition, amino acid and fatty acid profiles, and toxic elements contents of fanned and wild Chanodichthys mongolicus were established and compared. Significantly higher crude protein content while lower moisture content in farmed fish compared to wild fish were observed (P〈0.05). The percentages of total amino acids (TAA), total essential amino acids (TEAA), total non-essential amino acids (TNEAA) and total delicious amino acids (TDAA) in farmed fish were all significantly higher than those in the wild equivalent (P〈0.05). Compared to the FAO/WHO recommended reference values, the ratios of TEAA/ TAA (39.84%o-40.33%) were comparable to 40% and TEAA / TNEAA (66.22%-67.60%) were above 60%. Fatty acid profiles in both farmed and wild C. mongolicus were dominated by monounsaturated fatty acid (MUFA), with farmed fish contained much more MUFA content compared to wild counterpart (P〈0.05). Notably, wild fish exhibited significantly higher levels of total polyunsaturated fatty acid (PUFA) including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) than fanned fish (P〈0.05). The EPA (C20:503) and linoleic acid (C18:206) were the predominant PUFA in wild and farmed C. mongolicus, respectively. Moreover, farmed fish displayed an overall lower toxic element levels (As, Cd, Pb and Hg) in comparison with wild fish, and both were far lower than the established limit standard. In conclusion, our results suggest that the nutritional quality of fanned C. mongolicus was inferior to their wild counterpart with respect to fatty acids nutrition, and therefore further studies should focus on the improving C. mongolicus diet in order to enhance the overall nutritional composition.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2009AA06440,2013AA065805)the National Basic Research Program of China(973 Program)(No.2011CB2009001)the National Natural Science Foundation of China(No.31170337)
文摘Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients (nitrogen, phosphorus, silicon, and sulfur), light intensity and light path, on O. aurita cell growth and biochemical composition (protein, carbohydrate, β-1,3-glucan, lipids, and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of 15-1,3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.
文摘The macronutrient composition and in vitro protein digestibility of extruded fermented and unfermented extruded soya protein products (low, medium and high moisture), raw and fermented soya meal and soya kernel were studied. The protein content (g/100g soya dry weight) ranged from 38.20 to 62.98 with the highest content in the high moisture extruded protein product fermented with 5 mL inoculum of Bacillus natto. Contents of carbohydrates ranged from 14.77 to 29.08 while those of crude fibre, fat and ash were generally low. Fermentation better improved protein digestibility in the raw soya meal and kernel than in the unfermented extruded and extruded fermented products. SDS-PAGE electrophoresis revealed some degradation of the protein sub units of fermented samples.
基金Supported by Research Grant from National Science Council,Taipei, Taiwan, China No. 91-2815-C-038-007-B
文摘AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy. METHODS: Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gin. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis. RESULTS: The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6±242.5 vs-233.4±205.9 mg/d, d2, 31.8±238.8 vs-253.4±184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185±931 vs323±201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1β or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-α level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3. CONCLUSION: TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gin supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.
基金Supported by Major Project of Science and Technology in Guangxi Zhuang Autonomous Region(GK AA16380026)Special Fund for Innovation Team Building in Guangxi Zhuang Autonomous Region from Modern Agricultural Industry Technology System of China(nycytxgxcxtd-09-04)Project of Processing and Application Demonstration of High-yielding Forage Grass Products in South China(GYMK 201633019)~~
文摘This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise City in northern Guangxi to compare plant height, yield, stem-leaf ratio and nutrient composition am-ong Pennisetum purpureum Schumab cv. Purple, P. purpureum Schum cv. Guimin and P. purpureum Rich &#215; P. americana King grass cv. Reyan No.4. The results in-dicated that average annual fresh yield of P. purpureum Schumab cv. Purple reached 141.4 t/hm2, which was 2.3 t/hm^2 higher than that of P. purpureum_Rich×P. americana_King grass cv. Reyan No.4; both average annual fresh yield of these two species was lower than that of P. purpureum Schum cv. Guimin. Stem-leaf ratio of P. purpureum Schumab cv. Purple was the lowest (1.17), which was 17.8% lower compared with P. purpureum Schum cv. Guimin, with significant differences (P〈0.05), indicating that P. purpureum Schumab cv. Purple had more leaves. Crude protein content of P. purpureum_Schumab cv. Purple was slightly lower than that of P. purpureum Rich×P. americana_King grass cv. Reyan No.4, while P. purpureum Schumab cv. Purple was superior to P. purpureum Schum cv. Guimin and P. pur-pureum Rich ×P. americana_King grass cv. Reyan No.4 in other indicators. Specifi-cal y, P. purpureum Schumab cv. Purple contained 26.6% of dry matter, 39.1% of nitrogen-free extract and 2.9% of crude fat.
文摘The research works were carried out for determination of the washout, types, sorts and level of the development reasons of degradation with the purpose of preparation of the measures of fight against degradation in the soils in 2005-2009 in the lowland of Kur-Araz. The investigations were fulfilled by using of geographical and stasionar methods. It is revealed that 333.6 thous, ha of the soils of the lowland of Kur-Araz have been exposed to erosion. 97.4 thousand ha of them have been exposed to rainstorm erosion, 127.1 thous, have been exposed to irrigative and 122.1 thous, ha to wind erosion. The most dangerous of them are considered irrigative erosion. Under the influence of erosion process in deposited over the length of furrow, deposits the changes happened in maintenance of humus and nutritions elements. In dependence on slope and water expenditure on 1 ha 2.23-14.86 t of soil are leached. As a result of out wash from 1 ha of soil 55.88-304.59 kg of humus, 4.06-20.80 kg of nitrogen, 4.57-26.55 of phosphorus and 57.40-372.99 of potassium are lost. The soil is exposed to degradation and as a result a process of desertification begins. On the basis of the quantitative an intensity of soil outwash the possible losses of dry matter including humus and main elements of nutrition of plants as a result of erosion, their deposit with the water way, are calculated, too.