目的 获得可用于动物试验和临床试验的人表皮生长因子原料药。方法 化学合成的人表皮生长因子基因在甲基营养型酵母中表达并分泌到培养基中的人表皮生长因子蛋白经 Phenysepharose 6 Fast Flow（ high sub）柱、 Q-sepharose High Perfo...目的 获得可用于动物试验和临床试验的人表皮生长因子原料药。方法 化学合成的人表皮生长因子基因在甲基营养型酵母中表达并分泌到培养基中的人表皮生长因子蛋白经 Phenysepharose 6 Fast Flow（ high sub）柱、 Q-sepharose High Performance柱、 Superdex 30柱纯化后进行生物学性质检测。结果 纯化后的蛋白产物纯度达 98％，是无热源、无内毒素、无甲基营养型酵母染色体 DNA污染的，有正确的分子量、等电点、氨基末端氨基酸顺序、肽图、紫外光谱、及生物活性性质的重组蛋白。结论 纯化蛋白符合动物试验和临床试验的要求，为制备多种人表皮生长因子制剂进行动物实验和临床试验打下了基础。展开更多
Methylotrophic yeast expression system is one of the eukaryotic expression systems which was widely applied and devoloped fast in rencent years. Expression of the methanol inducible genes, expression vector, fermentat...Methylotrophic yeast expression system is one of the eukaryotic expression systems which was widely applied and devoloped fast in rencent years. Expression of the methanol inducible genes, expression vector, fermentation, purification and character of secretion proteins, factors affecting heterologous gene expression, comparision of methylotrophic yeast and other yeasts are reviewed in this paper. Ref展开更多
文摘Methylotrophic yeast expression system is one of the eukaryotic expression systems which was widely applied and devoloped fast in rencent years. Expression of the methanol inducible genes, expression vector, fermentation, purification and character of secretion proteins, factors affecting heterologous gene expression, comparision of methylotrophic yeast and other yeasts are reviewed in this paper. Ref
