Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor (BDNF) in rats with cerebral ischemia reperfusion injury (CIRI). Meth...Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor (BDNF) in rats with cerebral ischemia reperfusion injury (CIRI). Methods According to random number table, 48 SD rats were randomly divided into 6 groups, including normal control group (group A), sham operation group (group B), model group (group C), eye-acupuncture group (group D), non-acupoint of eye-acupuncture group (group E) and body-acupuncture group (group F), eight rats in each group. Artery infarction reperfusion model were prepared by using suture-occluded method. Liver region, upper energizer area, lower energizer area and kidney region were selected in the group D. Acupuncture was carried out at the point located at 3 mm from the acupoint areas in the group E. Qūchí (曲池 LI 11), Zúsānl (足三里 ST 36) and other acupoints were selected in the group F. Zea Longa scoring method was utilized for scoring the neural functions of rats; real-time PCR was carried out to examine the expression level of BDNF mRNA in the brain 72 h after ischemia reperfusion; western blot was carried out to examine the expression level of BDNF protein in the brain 72 h after ischemia reperfusion. Results The symptoms of neurologic impairments in the rats of the group D were alleviated in comparison to those in the group C (P0.01), and the difference between the group D and the group F was not statistically significant (P0.05); Compared with the group C, the mRNA and protein expression levels of BDNF in the brain of rats in the group D and the group F both increased (P0.01), but the difference between the group D and the group F was not statistically significant (P0.05). Conclusion The functions of eye-acupuncture and body-acupuncture in improving cerebral ischemia reperfusion injury are similar, and the functional mechanisms for the two different therapies may be related to the up-regulation of BDNF expression in brain and thus promote the repairing of brain tissues.展开更多
Objective To observe the effects of eye acupuncture on expression of brain-derived neurotrophic factor (BDNF) in the hippocampus of rats after ischemia- reperfusion injury in order to study the mechanisms of eye acu...Objective To observe the effects of eye acupuncture on expression of brain-derived neurotrophic factor (BDNF) in the hippocampus of rats after ischemia- reperfusion injury in order to study the mechanisms of eye acupuncture in improving ischemia-reperfusion injury. Methods SD rats were randomly divided into a normal group, a sham-operation group, a model group and an eye acupuncture group, with 8 rats in each. The cerebral ischemia-reperfusion injury model was established by thread occlusion method. Acupuncture at "liver", "upper-jiao", "lower-jiao", and "kidney" was performed for 20 min in the eye acupuncture group immediateness, 12 h and 23.5 h after the reperfusion. After 24 h of the reperfusion, the neurophysical behaviors were evaluated by Zea Longa neurophysical impairment score; the expression of ischemic hippocampus BDNF mRNA was measured by RT-PCR; the expression of ischemic hippocampus BDNF protein was detected by Western blot technique. Results After reperfusion 24 h, compared with the model group, the neurologic impairment score of the eye acupuncture group decreased significantly (P〈0.01); the expressions of BDNF mRNA and protein in rat hippocampi after the eye acupuncture therapy were both obviously increased (P〈0.01). Conclusion The eye acupuncture therapy is beneficial for neurofunctional rehabilitation by promoting the repair of the nerve cell which is induced by increasing hippocampus BDNF expression.展开更多
Objective:To observe the clinical effect of acupuncture plus rehabilitation training in treating early-stage shoulder-hand syndrome due to ischemic stroke.Methods:Sixty patients were randomized into an observation g...Objective:To observe the clinical effect of acupuncture plus rehabilitation training in treating early-stage shoulder-hand syndrome due to ischemic stroke.Methods:Sixty patients were randomized into an observation group and a control group,30 in each.The observation group was intervened by acupuncture plus rehabilitation training,and the control group only received rehabilitation training.After 3 treatment courses,the scores of visual analogue scale (VAS) and Fugl-Meyer assessment scale (FMA),and the clinical effect were compared between the two groups.Results:The two groups both obtained significant improvements in VAS and FMA scores after treatment (P〈0.05 or P〈0.01).After treatment,it showed marked differences in comparing VAS and FMA scores between the observation group and the control group (P〈0.05).The total effective rate was 86.7% in the observation group,versus 63.3% in the control group,and the difference was statistically significant (P〈0.05).Conclusion:The result of acupuncture plus rehabilitation training in treating early-stage shoulder-hand syndrome due to ischemic stroke is superior to rehabilitation training alone.展开更多
基金Supported by National Key Fundamental:Research and Development Project 2007 CB 512702A project of starting foundation for doctors in Liaoning Province:20131073
文摘Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor (BDNF) in rats with cerebral ischemia reperfusion injury (CIRI). Methods According to random number table, 48 SD rats were randomly divided into 6 groups, including normal control group (group A), sham operation group (group B), model group (group C), eye-acupuncture group (group D), non-acupoint of eye-acupuncture group (group E) and body-acupuncture group (group F), eight rats in each group. Artery infarction reperfusion model were prepared by using suture-occluded method. Liver region, upper energizer area, lower energizer area and kidney region were selected in the group D. Acupuncture was carried out at the point located at 3 mm from the acupoint areas in the group E. Qūchí (曲池 LI 11), Zúsānl (足三里 ST 36) and other acupoints were selected in the group F. Zea Longa scoring method was utilized for scoring the neural functions of rats; real-time PCR was carried out to examine the expression level of BDNF mRNA in the brain 72 h after ischemia reperfusion; western blot was carried out to examine the expression level of BDNF protein in the brain 72 h after ischemia reperfusion. Results The symptoms of neurologic impairments in the rats of the group D were alleviated in comparison to those in the group C (P0.01), and the difference between the group D and the group F was not statistically significant (P0.05); Compared with the group C, the mRNA and protein expression levels of BDNF in the brain of rats in the group D and the group F both increased (P0.01), but the difference between the group D and the group F was not statistically significant (P0.05). Conclusion The functions of eye-acupuncture and body-acupuncture in improving cerebral ischemia reperfusion injury are similar, and the functional mechanisms for the two different therapies may be related to the up-regulation of BDNF expression in brain and thus promote the repairing of brain tissues.
基金Supported by National Key Basic Research Development Project: 2007 CB 512702
文摘Objective To observe the effects of eye acupuncture on expression of brain-derived neurotrophic factor (BDNF) in the hippocampus of rats after ischemia- reperfusion injury in order to study the mechanisms of eye acupuncture in improving ischemia-reperfusion injury. Methods SD rats were randomly divided into a normal group, a sham-operation group, a model group and an eye acupuncture group, with 8 rats in each. The cerebral ischemia-reperfusion injury model was established by thread occlusion method. Acupuncture at "liver", "upper-jiao", "lower-jiao", and "kidney" was performed for 20 min in the eye acupuncture group immediateness, 12 h and 23.5 h after the reperfusion. After 24 h of the reperfusion, the neurophysical behaviors were evaluated by Zea Longa neurophysical impairment score; the expression of ischemic hippocampus BDNF mRNA was measured by RT-PCR; the expression of ischemic hippocampus BDNF protein was detected by Western blot technique. Results After reperfusion 24 h, compared with the model group, the neurologic impairment score of the eye acupuncture group decreased significantly (P〈0.01); the expressions of BDNF mRNA and protein in rat hippocampi after the eye acupuncture therapy were both obviously increased (P〈0.01). Conclusion The eye acupuncture therapy is beneficial for neurofunctional rehabilitation by promoting the repair of the nerve cell which is induced by increasing hippocampus BDNF expression.
文摘Objective:To observe the clinical effect of acupuncture plus rehabilitation training in treating early-stage shoulder-hand syndrome due to ischemic stroke.Methods:Sixty patients were randomized into an observation group and a control group,30 in each.The observation group was intervened by acupuncture plus rehabilitation training,and the control group only received rehabilitation training.After 3 treatment courses,the scores of visual analogue scale (VAS) and Fugl-Meyer assessment scale (FMA),and the clinical effect were compared between the two groups.Results:The two groups both obtained significant improvements in VAS and FMA scores after treatment (P〈0.05 or P〈0.01).After treatment,it showed marked differences in comparing VAS and FMA scores between the observation group and the control group (P〈0.05).The total effective rate was 86.7% in the observation group,versus 63.3% in the control group,and the difference was statistically significant (P〈0.05).Conclusion:The result of acupuncture plus rehabilitation training in treating early-stage shoulder-hand syndrome due to ischemic stroke is superior to rehabilitation training alone.