The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chr...The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.展开更多
文摘The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.
