One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were conf...One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 （68%） isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% （1911 05） of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 （or about 25%） of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II （57.8%） out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.展开更多
Milk is a food of great value, it provides more essential nutrient than any other natural food. The presence of pathogenic bacteria and antibiotic residues in milk can cause a real danger to consumers. Effectively, th...Milk is a food of great value, it provides more essential nutrient than any other natural food. The presence of pathogenic bacteria and antibiotic residues in milk can cause a real danger to consumers. Effectively, the milk consumption contaminated by bacteria can have an immediate impact which means a toxi-infection. Therefore, the presence of antibiotics residues in milk can constitute an important risk at the allergic and antibiotic resistance cases on the consumer. The present study concerning the pathogens germs identification and Antibiotic residues seeking in milk and their impact on the human health, has been realized on a total number of 80 samples of raw milk resulted from direct sale channel （dairies） throughout Blida different regions localities. The Microbiological analysis has shown only three conform samples to JORA Standards. Really, milk non-conformity results to the microbiological standards consisting on total aerobic mesophilic flora total count, total coliforms, Thermotolerant coliforms E. coli, Faecal streptococcus, Staphylococcus aureus have shown the following contamination rate： 61.25%, 93.75%, 86.25%, 55%, 93.75% and 50%. Salmonella is characterized by a total absence in all analyzed milk samples. Moreover, the antibiotics residues research by Delvotest SP make plainly visible 33 positive samples. Further, two samples of the three which were judged conform to the bacteriology standards has been found contaminated by the antibiotic residues. The analyzed milk quality can be considered as a real danger to the consumption.展开更多
文摘One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 （68%） isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% （1911 05） of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 （or about 25%） of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II （57.8%） out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.
文摘Milk is a food of great value, it provides more essential nutrient than any other natural food. The presence of pathogenic bacteria and antibiotic residues in milk can cause a real danger to consumers. Effectively, the milk consumption contaminated by bacteria can have an immediate impact which means a toxi-infection. Therefore, the presence of antibiotics residues in milk can constitute an important risk at the allergic and antibiotic resistance cases on the consumer. The present study concerning the pathogens germs identification and Antibiotic residues seeking in milk and their impact on the human health, has been realized on a total number of 80 samples of raw milk resulted from direct sale channel （dairies） throughout Blida different regions localities. The Microbiological analysis has shown only three conform samples to JORA Standards. Really, milk non-conformity results to the microbiological standards consisting on total aerobic mesophilic flora total count, total coliforms, Thermotolerant coliforms E. coli, Faecal streptococcus, Staphylococcus aureus have shown the following contamination rate： 61.25%, 93.75%, 86.25%, 55%, 93.75% and 50%. Salmonella is characterized by a total absence in all analyzed milk samples. Moreover, the antibiotics residues research by Delvotest SP make plainly visible 33 positive samples. Further, two samples of the three which were judged conform to the bacteriology standards has been found contaminated by the antibiotic residues. The analyzed milk quality can be considered as a real danger to the consumption.
