[Objective] The aim was to explore the photosynthetic characteristics of Turpan grapes under high temperature and strong light conditions. [Method] Using six grape varieties as experimental materials, the photosynthes...[Objective] The aim was to explore the photosynthetic characteristics of Turpan grapes under high temperature and strong light conditions. [Method] Using six grape varieties as experimental materials, the photosynthesis and fluorescence parameters of the grape varieties under high temperature and high light were determined. [Result] The results showed that Thompson Seedless showed strong selfprotection ability on the photosynthetic mechanism in the adversity, and lower photo inhibition on photosynthesis under high temperature and high light than other varieties in which Crimson Seedless was second place. Otherwise the damage on photosynthetic mechanism of Kyoho and Red Globe under the adversity environment is more dipper than the other varieties, and poor self-protection ability. [Conclusion]Thompson Seedless and Crimson Seedless had a strong adaptability for high temperature and strong light. But the adaptability of Kyoho and Red Globe were relatively poor.展开更多
After six years of Himrod grape regional trial, the Himrod had very early- ripening precocity, stable flower bud differentiation, good fruit quality and great resis- tance to diseases, which could be a moderate develo...After six years of Himrod grape regional trial, the Himrod had very early- ripening precocity, stable flower bud differentiation, good fruit quality and great resis- tance to diseases, which could be a moderate development grape variety in Tianjin. The introduction performance cultivation techniques of Himrod grape were summa- rized.展开更多
The study of leaves and their architecture evolution is important for understanding the fluid dynamics of water movement in /eaves. Recent studies have shown how these systems can be involved in the performance of phy...The study of leaves and their architecture evolution is important for understanding the fluid dynamics of water movement in /eaves. Recent studies have shown how these systems can be involved in the performance of physiological aspects, which are directly connected with the density of the vascular network and stomata per unit of surface area. The vein architecture, beyond being essential for a mechanical support of the leaf, can also play a crucial role in the efficiency of the photosynthesis. The aim of the present work was to highlight the possible role of leaves vein network as cooling system. The results support the hypothesis that the vascular system of grape leaves is correlated with leaf temperature.展开更多
Sperm motility is important in reproductive biology. To understand the physiological ecology characteristics of sperm of Sepiella maindroni, we studied the activation rate, active time, and lifespan of sperm to evalua...Sperm motility is important in reproductive biology. To understand the physiological ecology characteristics of sperm of Sepiella maindroni, we studied the activation rate, active time, and lifespan of sperm to evaluate the influence of external factors, including temperature, salinity, pH, glucose, NaCl, KCl, MgCl2, CaCl2, EDTA-2Na and artificial seawater on sperm motility. The results show that the appropriate activation and motility condition for sperm were： temperature 20-30℃ （optimum 25℃）, salinity 10.6-30.6 （optimum 15.9） and pH 8.0-8.6 （optimum 8.0-8.4）. Sperm activity varied in different concentrations of glucose, NaCl, KCl, MgCl2 and CaCl〉 None of the selected concentrations of EDTA-2Na solution could activate the sperm. The activation rate in artificial seawater devoid of Ca2＋, Mg2＋ or HCO3- was low. The results should help further studies on the preservation and activation of squid sperm.展开更多
An extracellular β-glucosidase produced by Aspergillus terreus was identified, purified, characterized and was tested for the hydrolysis of soybean isofiavone. Matrix-assisted laser desorption/ionization with tandem ...An extracellular β-glucosidase produced by Aspergillus terreus was identified, purified, characterized and was tested for the hydrolysis of soybean isofiavone. Matrix-assisted laser desorption/ionization with tandem time-of- flight/time-of-flight mass spectrometry （MALDI-TOF/TOF MS） revealed the protein to be a member of the glycosyl hydrolase family 3 with an apparent molecular mass of about 120 kDa. The purified 13-glucosidase showed optimal activity at pH 5.0 and 65℃ and was very stable at 50℃. Moreover, the enzyme exhibited good stability over pH 3.0-8.0 and possessed high tolerance towards pepsin and trypsin. The kinetic parameters Km （apparent Michaelis- Menten constant） and Vmax （maximal reaction velocity） for p-nitrephenyl-β-D-cjlucopyranoside （pNPG） were 1.73 mmol/L and 42.37 U/mg, respectively. The Krn and Vmax for cellobiose were 4.11 mmol/L and 5.7 U/mg, respectively. The enzyme efficiently converted isoflavone glycosides to aglycones, with a hydrolysis rate of 95.8% for daidzin, 86.7% for genistin, and 72.1% for glycitin. Meanwhile, the productivities were 1.14 mmol/（L.h） for daidzein, 0.72 mmol/（L.h） for genistein, and 0.19 mmol/（L.h） for glycitein. This is the first report on the application of A. terreus β-glucosidase for converting isoflavone glycosides to their aglycones in soybean products.展开更多
A colorimetric method has been established for a-glucosidase activity assay and its inhibitor screening. The method is based on the specific recognition between 1,4-phenylenediboronic acid （PDBA） and 4-aminophenyl-a...A colorimetric method has been established for a-glucosidase activity assay and its inhibitor screening. The method is based on the specific recognition between 1,4-phenylenediboronic acid （PDBA） and 4-aminophenyl-a-D-glucopyranoside （pAPG）, which may induce aggregation of pAPG-functionalized gold nano- particles （AuNPs） to achieve color change of the test solution. Because pAPG is the substrate of α-glucosidase, the aggregation of AuNPs will be influenced by α-glucosidase since there is no coordination reactivity between PDBA and 4-aminobenzene, the hydrolyzed product of pAPG catalyzed by the enzyme. Therefore, a simple and easily-operated colorimetric method for the assay of a-glucosidase activity can be developed. Under the optimized experimental conditions, the ratios of absorbance at a wavelength of 650 nm to that at 520 nm vary linearly with the α-glucosidase activity within a range from 0.05 to 1.1 U/mL with a lowest detection limit of 0.004 U/mL. Moreover, using the proposed method, the inhibition effect of gallic acid and quercetin on a-glucosidase activity can be tested with IC50 values of 1.16 mM and 1.82 μM, respectively. Thus, the method has a great potential not only for the detection of a-glucosidase activity, but also for the screening of its inhibitors.展开更多
基金Supported by the Key of Xinjiang Characteristic Fruit Efficient Safety Production Technology Integration and Demonstration of Science and Technology Plan Projects in Xinjiang Uygur Autonomous Region(201130102-1)Modern Agricultural Industry Technology System Special Funding(CARS-30)Xin Jiang Uygur Autonomous Region Fruit Trees Key Subject~~
文摘[Objective] The aim was to explore the photosynthetic characteristics of Turpan grapes under high temperature and strong light conditions. [Method] Using six grape varieties as experimental materials, the photosynthesis and fluorescence parameters of the grape varieties under high temperature and high light were determined. [Result] The results showed that Thompson Seedless showed strong selfprotection ability on the photosynthetic mechanism in the adversity, and lower photo inhibition on photosynthesis under high temperature and high light than other varieties in which Crimson Seedless was second place. Otherwise the damage on photosynthetic mechanism of Kyoho and Red Globe under the adversity environment is more dipper than the other varieties, and poor self-protection ability. [Conclusion]Thompson Seedless and Crimson Seedless had a strong adaptability for high temperature and strong light. But the adaptability of Kyoho and Red Globe were relatively poor.
基金Supported by Key Program of Applied Basic and Frontier Technology Research Foundation of Tianjin(14JCZDJC34400)Special Fund for Modern Agricultural Technology System(CARS-30)Transformation and Extension Of Agricultural Science and Technology Achievements in Tianjin(201304120)~~
文摘After six years of Himrod grape regional trial, the Himrod had very early- ripening precocity, stable flower bud differentiation, good fruit quality and great resis- tance to diseases, which could be a moderate development grape variety in Tianjin. The introduction performance cultivation techniques of Himrod grape were summa- rized.
文摘The study of leaves and their architecture evolution is important for understanding the fluid dynamics of water movement in /eaves. Recent studies have shown how these systems can be involved in the performance of physiological aspects, which are directly connected with the density of the vascular network and stomata per unit of surface area. The vein architecture, beyond being essential for a mechanical support of the leaf, can also play a crucial role in the efficiency of the photosynthesis. The aim of the present work was to highlight the possible role of leaves vein network as cooling system. The results support the hypothesis that the vascular system of grape leaves is correlated with leaf temperature.
基金Supported by the National Natural Science Foundation of China (No.40646030)the National Agriculture Science and Technology Achievements Transfer Capital (No.2009GB2C2204)the Major Project of Zhejiang Province (No.2007C12061)
文摘Sperm motility is important in reproductive biology. To understand the physiological ecology characteristics of sperm of Sepiella maindroni, we studied the activation rate, active time, and lifespan of sperm to evaluate the influence of external factors, including temperature, salinity, pH, glucose, NaCl, KCl, MgCl2, CaCl2, EDTA-2Na and artificial seawater on sperm motility. The results show that the appropriate activation and motility condition for sperm were： temperature 20-30℃ （optimum 25℃）, salinity 10.6-30.6 （optimum 15.9） and pH 8.0-8.6 （optimum 8.0-8.4）. Sperm activity varied in different concentrations of glucose, NaCl, KCl, MgCl2 and CaCl〉 None of the selected concentrations of EDTA-2Na solution could activate the sperm. The activation rate in artificial seawater devoid of Ca2＋, Mg2＋ or HCO3- was low. The results should help further studies on the preservation and activation of squid sperm.
基金Project supported by the Innovation Team Program of Zhejiang Province(No.2011R50025-12),China
文摘An extracellular β-glucosidase produced by Aspergillus terreus was identified, purified, characterized and was tested for the hydrolysis of soybean isofiavone. Matrix-assisted laser desorption/ionization with tandem time-of- flight/time-of-flight mass spectrometry （MALDI-TOF/TOF MS） revealed the protein to be a member of the glycosyl hydrolase family 3 with an apparent molecular mass of about 120 kDa. The purified 13-glucosidase showed optimal activity at pH 5.0 and 65℃ and was very stable at 50℃. Moreover, the enzyme exhibited good stability over pH 3.0-8.0 and possessed high tolerance towards pepsin and trypsin. The kinetic parameters Km （apparent Michaelis- Menten constant） and Vmax （maximal reaction velocity） for p-nitrephenyl-β-D-cjlucopyranoside （pNPG） were 1.73 mmol/L and 42.37 U/mg, respectively. The Krn and Vmax for cellobiose were 4.11 mmol/L and 5.7 U/mg, respectively. The enzyme efficiently converted isoflavone glycosides to aglycones, with a hydrolysis rate of 95.8% for daidzin, 86.7% for genistin, and 72.1% for glycitin. Meanwhile, the productivities were 1.14 mmol/（L.h） for daidzein, 0.72 mmol/（L.h） for genistein, and 0.19 mmol/（L.h） for glycitein. This is the first report on the application of A. terreus β-glucosidase for converting isoflavone glycosides to their aglycones in soybean products.
文摘A colorimetric method has been established for a-glucosidase activity assay and its inhibitor screening. The method is based on the specific recognition between 1,4-phenylenediboronic acid （PDBA） and 4-aminophenyl-a-D-glucopyranoside （pAPG）, which may induce aggregation of pAPG-functionalized gold nano- particles （AuNPs） to achieve color change of the test solution. Because pAPG is the substrate of α-glucosidase, the aggregation of AuNPs will be influenced by α-glucosidase since there is no coordination reactivity between PDBA and 4-aminobenzene, the hydrolyzed product of pAPG catalyzed by the enzyme. Therefore, a simple and easily-operated colorimetric method for the assay of a-glucosidase activity can be developed. Under the optimized experimental conditions, the ratios of absorbance at a wavelength of 650 nm to that at 520 nm vary linearly with the α-glucosidase activity within a range from 0.05 to 1.1 U/mL with a lowest detection limit of 0.004 U/mL. Moreover, using the proposed method, the inhibition effect of gallic acid and quercetin on a-glucosidase activity can be tested with IC50 values of 1.16 mM and 1.82 μM, respectively. Thus, the method has a great potential not only for the detection of a-glucosidase activity, but also for the screening of its inhibitors.
