[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phyl...[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database.[Result] Six G6PDH genes,including one cytomic type and five plastids,were detected in the E.grandsis genome.All the G6PDHs have conserved motifs of motif 1,motif 2,motif 3,motif 7,motif 9 and motif 11.Furthermore,promoter sequences of all E.grandsis G6PDH contain TATA box,enhancer,light-responsive,hormone-responsive and stress-responsive regulatory elements.[Conclusion] This study provided reference for the further revealing molecular function of E.grandsis G6PDH gene family展开更多
Yeast strain Y68 producing high level of pullulan was isolated from the phyton collected in Toulouse, France. This strain was identified to be Rhodotorula bacarum by BIOLOG analysis. This is the first report that pull...Yeast strain Y68 producing high level of pullulan was isolated from the phyton collected in Toulouse, France. This strain was identified to be Rhodotorula bacarum by BIOLOG analysis. This is the first report that pullulan was produced by Rhodotorula bacarum. The optimal medium (g L -1) for pullulan production by this strain was 80 glucose, 20 soybean cake hydrolysate, 5 K 2HPO 4, 1 NaCl, 0.2 MgSO 4·7H 2O, 0.6 (NH 4) 2SO 4, pH 7.0. Under this condition, 54 g L -1 pullulan was produced within 60 h at 30 ℃. Pullulan is a better starting material for producing marine prodrugs.展开更多
Diabetes mellitus （DM） is associated with increased oxidative stress due to elevated glucose levels in the plasma. Glucose promotes glycosylation of both plasma and cellular proteins with increased risk for vascular...Diabetes mellitus （DM） is associated with increased oxidative stress due to elevated glucose levels in the plasma. Glucose promotes glycosylation of both plasma and cellular proteins with increased risk for vascular events. Diabetic patients suffer from a higher incidence of cardiovascular complications such as diabetic ne-phropathy. Haptoglobin （Hp） is an antioxidant plasma protein which binds free hemoglobin, thus preventing heme-iron mediated oxidation. Two alleles exist at the Hp gene locus （1 and 2） encoding three possible Hp genotypes that differ in their antioxidant ability, and may respond differently to vitamin E treatment. Several clinical studies to have shown that Hp 1-1 genotype is a superior antioxidant to the Hp 2-2 genotype and Hp 2-2 genotype is associated with a higher incidence of cardiovascular disease. Vitamin E was found to have benefcial effect in patient and mice with Hp 2-2 geno-type. In this review we have summarized the results of our studies in patients with diabetic nephropathy treated with vitamin E and in diabetic mice with differ-ent haptoglobin genotypes.展开更多
A new adsorbent for heavy metal ions, sulfhydryl chitosan (S chitosan), was produced by treatment of chitosan with sulhydryl acetic acid in the presence of sulfuric acid as a catalyst. Its structure was confirmed by e...A new adsorbent for heavy metal ions, sulfhydryl chitosan (S chitosan), was produced by treatment of chitosan with sulhydryl acetic acid in the presence of sulfuric acid as a catalyst. Its structure was confirmed by elemental analysis and FT IR spectra analysis. The adsorption properties of sulfhydryl chitosan for Cu(Ⅱ), Cd(Ⅱ), Pb(Ⅱ), Cr(Ⅲ) and Ni(Ⅱ) were investigated, and the effect of pH value on adsorption, adsorption kinetics, and selective adsorption was examined. It was shown that S chitosan has good adsorption for Pb(Ⅱ), Cu(Ⅱ) and Cd(Ⅱ) like chitosan, is also insoluble in acid solution; has good adsorption kinetic properties for heavy metal ions; and can be used in acid solution. The adsorption capacities of S chitosan can be affected by media acidity. The adsorbed Cu(Ⅱ) Cd(Ⅱ)and Pb(Ⅱ) could be eluted by diluted chlorhydric acid.展开更多
In this work, aerial parts of Euphorbia guyoniana were studied. The use of preparative high pressure liquid chromatography allowed to isolate two flavonoids and a gallic tannins： Quercetine-3 O-β-D-glucuronide, kaem...In this work, aerial parts of Euphorbia guyoniana were studied. The use of preparative high pressure liquid chromatography allowed to isolate two flavonoids and a gallic tannins： Quercetine-3 O-β-D-glucuronide, kaempferol-30-β-D-glucuronide and 1, 2, 6 tri-O-galloyl-β-D-glucose. Identifications were carried out using spectroscopic methods. In addition, scavenger potential of these compounds was studied using DPPH test.展开更多
Background and aims： The comparative efficacy of glucose solution （GS） versus non-nutritive sucking （NNS） for pain re- lief in neonate remains controversial. This systematic review was consequently performed to o...Background and aims： The comparative efficacy of glucose solution （GS） versus non-nutritive sucking （NNS） for pain re- lief in neonate remains controversial. This systematic review was consequently performed to objectively investigate the analgesic effect of Oral GS related to NNS in newborns during nociceptive procedures. Methods： All potential records were searched in PubMed, Web of Science, EMBASE, and the Cochrane Library to capture the randomized controlled trials comparing GS with NNS in terms of pain scores in neonates through January 2017. Two independent investigators screened the identified articles, extracted the data, and assessed the methodological quality of the included studies. All statistical analyses were completed by using Review Manager （RevMan） version 5.3.0. Results： Four studies, involving 248 neonates, were incorporated into these statistical analyses. For reducing pain scores, no statistical difference was detected when GS compared to NNS （4 trials; mean difference [MD], 0.75; 95% confidence interval [CI], -0.77 to 2.27; P = 0.33）. Conclusion： Both the GS and NNS reduced the pain scores in neonates who undergoing Hepatitis B vaccination and venipuncture, but the NNS is more convenience than GS to some extent. Considered the limited evidences, more randomized controlled trials with high-quality, large-scale and appropriate measures time are warranted to further establish the comparative efficacy of these two options.展开更多
Routine chemical examination of urine has changed dramatically since the early days of urine testing, owing to the development of the reagent strip method for chemical analysis. Reagent strips currently provide a simp...Routine chemical examination of urine has changed dramatically since the early days of urine testing, owing to the development of the reagent strip method for chemical analysis. Reagent strips currently provide a simple, rapid means for performing medically significant chemical analysis of urine, including pH, protein, glucose, ketones, blood, bilirubin, urobilinogen, nitrite, leukocytes, and specific gravity. The study was conducted in the Medical Hospital in Berat City. A total of 480 midstream urine specimens were collected in sterile containers from urinalysis cases of women （reproductive age group） from outdoor and indoor female patients （n = 480）. A total of 240 pregnant women and 240 non-pregnant women were evaluated. The patients included were between the ages of 15-50 years. The obtained results in this study ware： 1） The percentage of glucose in pregnant women has resulted in 6.67%, while in non-pregnant women 23.34% distributed by age group; 2） 16% of pregnant women had bilirubinuria, where the highest percentage is occupied by 1＋, while in non-pregnant women 26%, where the highest percentage is occupied by 1＋; 3） Urobilinogen in pregnant women has this distribution： 11.67% of the cases included in the amount of 0.7, 82% of the cases included in the amount of 1 and only 6.34% of the quantity 1.5. Urobilinogen in non-pregnant women： 11.67% of cases are in the amount 0.7 and 72.92% of the quantity 1, while others are scattered. The material was collected in sterile cups for urine. The collected material was processed based on evidence to beakers or microscope preparations. In 5 age groups, the highest percentage is from 26 to 30 years old in the pregnant women and for the non-pregnant women 15 to 20 years old.展开更多
β-Glucuronidase(GUS)plays a key role in tumor initiation,metastasis,and progression,and thus,has been proposed as a promising cancer biomarker.In this study,we designed an enzyme-activatable near-infrared(NIR)fluores...β-Glucuronidase(GUS)plays a key role in tumor initiation,metastasis,and progression,and thus,has been proposed as a promising cancer biomarker.In this study,we designed an enzyme-activatable near-infrared(NIR)fluorescent probe(DCM-βGlcA)for the rapid and accurate detection of GUS activity in vitro,in vivo and ex vivo.The DCM-βGlcA was prepared by linking a glucuronic acid residue to dicyanomethylene-4 H-pyran(DCM).This probe exhibited significant light-up NIR fluorescent signals at 680 nm after reacting with GUS and the Stokes shift could reach 150 nm.The DCM-βGlcA showed a high sensitivity toward GUS and an excellent linear relationship at concentrations ranging between 0 and 4 U L^(-1)(R^(2)=0.9974)with the limit of detection as low as 0.19 U L^(-1).We used the DCM-βGlcA to identify GUS serum levels in both cancer patients and healthy individuals with a similar accuracy as that of an enzyme-linked immunosorbent assay(ELISA)while being easier and faster to perform.Moreover,the DCM-βGlcA was used for tracking endogenous GUS in living cells,thereby discriminating GUSoverexpressed liver cancer from normal cells.Additionally,the DCM-βGlcA was able to detect and image endogenous GUS in liver cancer tissue and tumor-bearing mouse models.These findings demonstrate the potential of the DCM-βGlcA as a promising tool for detecting and monitoring GUS activity in preclinical applications.展开更多
基金Supported by Seeding Raising Project from Guangdong Provincial Department(LYM10040)Open Research Project of Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,MOE,Beijing Forestry University(FOP2010-4)~~
文摘[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database.[Result] Six G6PDH genes,including one cytomic type and five plastids,were detected in the E.grandsis genome.All the G6PDHs have conserved motifs of motif 1,motif 2,motif 3,motif 7,motif 9 and motif 11.Furthermore,promoter sequences of all E.grandsis G6PDH contain TATA box,enhancer,light-responsive,hormone-responsive and stress-responsive regulatory elements.[Conclusion] This study provided reference for the further revealing molecular function of E.grandsis G6PDH gene family
基金National Natural Science Foundation of China(Grant No.39970005)for its financial support
文摘Yeast strain Y68 producing high level of pullulan was isolated from the phyton collected in Toulouse, France. This strain was identified to be Rhodotorula bacarum by BIOLOG analysis. This is the first report that pullulan was produced by Rhodotorula bacarum. The optimal medium (g L -1) for pullulan production by this strain was 80 glucose, 20 soybean cake hydrolysate, 5 K 2HPO 4, 1 NaCl, 0.2 MgSO 4·7H 2O, 0.6 (NH 4) 2SO 4, pH 7.0. Under this condition, 54 g L -1 pullulan was produced within 60 h at 30 ℃. Pullulan is a better starting material for producing marine prodrugs.
文摘Diabetes mellitus （DM） is associated with increased oxidative stress due to elevated glucose levels in the plasma. Glucose promotes glycosylation of both plasma and cellular proteins with increased risk for vascular events. Diabetic patients suffer from a higher incidence of cardiovascular complications such as diabetic ne-phropathy. Haptoglobin （Hp） is an antioxidant plasma protein which binds free hemoglobin, thus preventing heme-iron mediated oxidation. Two alleles exist at the Hp gene locus （1 and 2） encoding three possible Hp genotypes that differ in their antioxidant ability, and may respond differently to vitamin E treatment. Several clinical studies to have shown that Hp 1-1 genotype is a superior antioxidant to the Hp 2-2 genotype and Hp 2-2 genotype is associated with a higher incidence of cardiovascular disease. Vitamin E was found to have benefcial effect in patient and mice with Hp 2-2 geno-type. In this review we have summarized the results of our studies in patients with diabetic nephropathy treated with vitamin E and in diabetic mice with differ-ent haptoglobin genotypes.
文摘A new adsorbent for heavy metal ions, sulfhydryl chitosan (S chitosan), was produced by treatment of chitosan with sulhydryl acetic acid in the presence of sulfuric acid as a catalyst. Its structure was confirmed by elemental analysis and FT IR spectra analysis. The adsorption properties of sulfhydryl chitosan for Cu(Ⅱ), Cd(Ⅱ), Pb(Ⅱ), Cr(Ⅲ) and Ni(Ⅱ) were investigated, and the effect of pH value on adsorption, adsorption kinetics, and selective adsorption was examined. It was shown that S chitosan has good adsorption for Pb(Ⅱ), Cu(Ⅱ) and Cd(Ⅱ) like chitosan, is also insoluble in acid solution; has good adsorption kinetic properties for heavy metal ions; and can be used in acid solution. The adsorption capacities of S chitosan can be affected by media acidity. The adsorbed Cu(Ⅱ) Cd(Ⅱ)and Pb(Ⅱ) could be eluted by diluted chlorhydric acid.
文摘In this work, aerial parts of Euphorbia guyoniana were studied. The use of preparative high pressure liquid chromatography allowed to isolate two flavonoids and a gallic tannins： Quercetine-3 O-β-D-glucuronide, kaempferol-30-β-D-glucuronide and 1, 2, 6 tri-O-galloyl-β-D-glucose. Identifications were carried out using spectroscopic methods. In addition, scavenger potential of these compounds was studied using DPPH test.
文摘Background and aims： The comparative efficacy of glucose solution （GS） versus non-nutritive sucking （NNS） for pain re- lief in neonate remains controversial. This systematic review was consequently performed to objectively investigate the analgesic effect of Oral GS related to NNS in newborns during nociceptive procedures. Methods： All potential records were searched in PubMed, Web of Science, EMBASE, and the Cochrane Library to capture the randomized controlled trials comparing GS with NNS in terms of pain scores in neonates through January 2017. Two independent investigators screened the identified articles, extracted the data, and assessed the methodological quality of the included studies. All statistical analyses were completed by using Review Manager （RevMan） version 5.3.0. Results： Four studies, involving 248 neonates, were incorporated into these statistical analyses. For reducing pain scores, no statistical difference was detected when GS compared to NNS （4 trials; mean difference [MD], 0.75; 95% confidence interval [CI], -0.77 to 2.27; P = 0.33）. Conclusion： Both the GS and NNS reduced the pain scores in neonates who undergoing Hepatitis B vaccination and venipuncture, but the NNS is more convenience than GS to some extent. Considered the limited evidences, more randomized controlled trials with high-quality, large-scale and appropriate measures time are warranted to further establish the comparative efficacy of these two options.
文摘Routine chemical examination of urine has changed dramatically since the early days of urine testing, owing to the development of the reagent strip method for chemical analysis. Reagent strips currently provide a simple, rapid means for performing medically significant chemical analysis of urine, including pH, protein, glucose, ketones, blood, bilirubin, urobilinogen, nitrite, leukocytes, and specific gravity. The study was conducted in the Medical Hospital in Berat City. A total of 480 midstream urine specimens were collected in sterile containers from urinalysis cases of women （reproductive age group） from outdoor and indoor female patients （n = 480）. A total of 240 pregnant women and 240 non-pregnant women were evaluated. The patients included were between the ages of 15-50 years. The obtained results in this study ware： 1） The percentage of glucose in pregnant women has resulted in 6.67%, while in non-pregnant women 23.34% distributed by age group; 2） 16% of pregnant women had bilirubinuria, where the highest percentage is occupied by 1＋, while in non-pregnant women 26%, where the highest percentage is occupied by 1＋; 3） Urobilinogen in pregnant women has this distribution： 11.67% of the cases included in the amount of 0.7, 82% of the cases included in the amount of 1 and only 6.34% of the quantity 1.5. Urobilinogen in non-pregnant women： 11.67% of cases are in the amount 0.7 and 72.92% of the quantity 1, while others are scattered. The material was collected in sterile cups for urine. The collected material was processed based on evidence to beakers or microscope preparations. In 5 age groups, the highest percentage is from 26 to 30 years old in the pregnant women and for the non-pregnant women 15 to 20 years old.
基金supported by the National Natural Science Foundation of China(81801858)China Postdoctoral Science Foundation(2018M641676)the Applied Basic Research Program of Shanxi Province(201801D221408)。
文摘β-Glucuronidase(GUS)plays a key role in tumor initiation,metastasis,and progression,and thus,has been proposed as a promising cancer biomarker.In this study,we designed an enzyme-activatable near-infrared(NIR)fluorescent probe(DCM-βGlcA)for the rapid and accurate detection of GUS activity in vitro,in vivo and ex vivo.The DCM-βGlcA was prepared by linking a glucuronic acid residue to dicyanomethylene-4 H-pyran(DCM).This probe exhibited significant light-up NIR fluorescent signals at 680 nm after reacting with GUS and the Stokes shift could reach 150 nm.The DCM-βGlcA showed a high sensitivity toward GUS and an excellent linear relationship at concentrations ranging between 0 and 4 U L^(-1)(R^(2)=0.9974)with the limit of detection as low as 0.19 U L^(-1).We used the DCM-βGlcA to identify GUS serum levels in both cancer patients and healthy individuals with a similar accuracy as that of an enzyme-linked immunosorbent assay(ELISA)while being easier and faster to perform.Moreover,the DCM-βGlcA was used for tracking endogenous GUS in living cells,thereby discriminating GUSoverexpressed liver cancer from normal cells.Additionally,the DCM-βGlcA was able to detect and image endogenous GUS in liver cancer tissue and tumor-bearing mouse models.These findings demonstrate the potential of the DCM-βGlcA as a promising tool for detecting and monitoring GUS activity in preclinical applications.
