A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and ...A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃.展开更多
The platinum nanoparticles were adsorbed on graphene oxide sheets and played an important role in catalytic reduction of graphene oxide with hydrazine, leading to the formation of graphene-Pt nanoparticles. Because of...The platinum nanoparticles were adsorbed on graphene oxide sheets and played an important role in catalytic reduction of graphene oxide with hydrazine, leading to the formation of graphene-Pt nanoparticles. Because of their good electronic properties, biocompatibility and high surface area, graphene-Pt based composites achieved the direct electron transfer of redox enzyme and maintained their bioactivity well. The graphene-Pt nanocomposites were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM) and selected area electron diffraction (SAED). The amperometric biosensor fabricated by depositing glucose oxidase over Nafion-solubilized graphene-Pt electrode retained its biocatalytic activity and has offered fast and sensitive glucose quantification.展开更多
基金Projects(50473022, 20673036) supported by the National Natural Science Foundation of China project(2005) supported by the State Key Laboratory of Chemo/Biosensing and Chemometrics of China+1 种基金 project(2006FJ4100) supported by the Science Technology Project of Hunan Province project(2006) supported by the Postdoctor Foundation of Hunan University
文摘A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃.
基金supported by the National Natural Science Foundation of China (Grant No. 21003151)the Fundamental Research Funds for the Central Universities (Grant No. 0400219212)
文摘The platinum nanoparticles were adsorbed on graphene oxide sheets and played an important role in catalytic reduction of graphene oxide with hydrazine, leading to the formation of graphene-Pt nanoparticles. Because of their good electronic properties, biocompatibility and high surface area, graphene-Pt based composites achieved the direct electron transfer of redox enzyme and maintained their bioactivity well. The graphene-Pt nanocomposites were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM) and selected area electron diffraction (SAED). The amperometric biosensor fabricated by depositing glucose oxidase over Nafion-solubilized graphene-Pt electrode retained its biocatalytic activity and has offered fast and sensitive glucose quantification.
