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Isolation, Phenotypic Identification and Fingerprint by RAPD-PCR of Oxalotrophoic Burkholderia cepacia from Egyptian Fertile Soil
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作者 Hala Abou Shady Mona Kilany Abd EI Gawad +1 位作者 Qadria Genedi Wafaa Farouk 《Journal of Life Sciences》 2012年第7期732-739,共8页
Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egypti... Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egyptian fertile soil was aimed in this work. Isolated bacteria were first characterized using API 20 NE system. Nine RAPD primers were used in genetic fingerprinting of the characterized bacteria using RAPD-PCR. The preliminary characterization and polyphasic taxonomy for mesophilic, aerobic oxalate-degrading bacteria isolated from Egyptian fertile soil including auxanography using API galleries confirmed that the isolate belonged to the genus Burkholderia cepacia, the percentage of identity was 99.8% for 5167777 profiles. Fingerprinting by RAPD-PCR showed a DNA fingerprint ranging from 0 to 9 bands, over a size range of 344 bp to 3,280 bp. 展开更多
关键词 Burkholderia cepacia oxalotrophic RAPD-PCR FINGERPRINT API 20 NE ISOLATION identification.
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Toxicity Screening of Water Sources in Flooded Agricultural Areas of Nen-Shkodra Lowland Using Allium cepa L. Assay
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作者 Ditika Kopliku 《Journal of Environmental Science and Engineering(A)》 2012年第10期1197-1202,共6页
The reaction ofAllium cepa root tips to the presence of potential toxic chemicals was used to evaluate the cyto/genotoxic effects of some water sources of Nen-Shkodra lowland agricultural area after massive 2010-2011 ... The reaction ofAllium cepa root tips to the presence of potential toxic chemicals was used to evaluate the cyto/genotoxic effects of some water sources of Nen-Shkodra lowland agricultural area after massive 2010-2011 flooding. MRL (Mean root length), MI (mitotic index), PI (phase index) and CA (chromosome abnormalities) endpoints of onion roots, grown in four water samples, were measured and compared with tap water and Cu solution 0.5 mg/L (negative and positive controls). Inhibition of root growth, cell division and induction of mitotic and chromosomal aberrations were detected. The most polluted water sample caused MRL inhibition of 36%, MI decrease of 38%, and a considerable increase in chromosomal aberrations (7.8%) compared to the negative control. Most frequent CA types were: bridges and fragments, stickiness and C-metaphase. The results indicated a slight toxic tendency of analyzed natural waters, serving as an alert of environmental impact that chemical pollution had after flooding. 展开更多
关键词 Agriculture water sources Allium cepa assay cyto/genotoxicity
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行途烟驿
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作者 烟驿 《走向世界》 2019年第9期82-87,共6页
葡醍海湾与养马岛再次回到龙湖.葡醍海湾,大海已经退潮。金秋阳光洒在这个童话般的花园小区,一切恍若仙境。红色小火车是骄傲的小骏马,停在马路边等待这群特殊游客。“全国知名作家学者看烟台”采风团一行十几人,穿过绿草如茵,花果飘香... 葡醍海湾与养马岛再次回到龙湖.葡醍海湾,大海已经退潮。金秋阳光洒在这个童话般的花园小区,一切恍若仙境。红色小火车是骄傲的小骏马,停在马路边等待这群特殊游客。“全国知名作家学者看烟台”采风团一行十几人,穿过绿草如茵,花果飘香的别墅区,乘坐小骏马做一次“偷得浮生半日闲”的畅游。尽管时间并不能偷来,却不影响从仙女葱指拨动竖琴的密集音符中,摘出明亮的一粒,镶嵌于大海与绿色描画的一日上。对于美,一切耗费都是值的,而我又喜欢跟随美的物事,把自己消磨其中。宽阔柏油路面平展在绿山坡之间,小桥流水静谧. 展开更多
关键词 仙境 烟驿 别墅区 海湾 平展 行途 葱指 骏马
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