DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbit...DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.展开更多
[Objective] To study the preparation of Zipu Capsule and its clinical effect on the treatment of chronic periodontitis. [Method] The extraction, concentration, drying and preparation process of the raw herbal material...[Objective] To study the preparation of Zipu Capsule and its clinical effect on the treatment of chronic periodontitis. [Method] The extraction, concentration, drying and preparation process of the raw herbal materials for Zipu Capsule were selected out. And 60 cases of chronic periodontitis patients were randomly assigned to two groups and were treated with conventional therapy and nursing care, while the treatment group was added with taking the Zipu capsule, to compare the cura- tive effects of the two groups. [Result] Both of two groups showed decreases in gingival index （GO, periodontal pocket depth （PD） and attachment loss values （AL） after treatment, and those of the treatment group was significantly lower than those of the control group （P〈0.05）. The total curative effective rate of the treatment group was 91.17%, and 76.92% in the control group, and the differences between the two groups reached the significant level （P〈0.05）. [Conclusion] Zipu Capsule had definite therapeutic effect in treatment of chronic periodontitis and less adverse re- actions, which laid the foundation for the further development into drug products.展开更多
[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan...[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.展开更多
This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） w...This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.展开更多
To evaluate the effect of tectonic deformation on coal reservoir properties, we provide an analysis of the types of tectonically deformed coal, macroand microscopic deformation and discuss pore structural characterist...To evaluate the effect of tectonic deformation on coal reservoir properties, we provide an analysis of the types of tectonically deformed coal, macroand microscopic deformation and discuss pore structural characteristics and connectivity based on samples from the Puhe and Shanchahe coal mines. Our research shows that the tectonically deformed coal mostly includes cataclastic structural coal, mortar structural coal and schistose structural coal of a brittle deformation series. The major pore structures of different types of tectonically deformed coal are transitional pores and micropores. The pore volumes of macropores and visible fracture pores produced by structural deformations vary over a large range and increase with the intensity of tectonic deformation. Mesopores as connecting passages develop well in schistose structural coal. According to the shapes of intrusive mercury curves, tectonically deformed coal can be divided into parallel, open and occluded types. The parallel type has poor connectivity and is relatively closed; the open type reflects uniformly developed open pores with good connectivity while the occluded type is good for coalbed methane enrichment, but has poor connectivity between pores.展开更多
To probe the influence and the adverse-resistance characteristics of wetland plants in presence of silver nanoparticles (AgNPs), the changes in the physiological and biochemical characteristics (including the superoxi...To probe the influence and the adverse-resistance characteristics of wetland plants in presence of silver nanoparticles (AgNPs), the changes in the physiological and biochemical characteristics (including the superoxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity, soluble protein content, and chlorophyll content) of Typha orientalis exposed to different concentrations of AgNPs solutions (0, 0.1, 1, 20 and 40 mg/L) were explored. Meantime, the accumulation of silver content in these plants was revealed. The results show that under low concentrations of AgNPs, the SOD and POD activities in the leaves of Typha orientalis are strengthened to different degrees. However, high concentrations of AgNPs inhibit the activities of SOD and POD. Under the stress of different concentrations of AgNPs, the CAT activities are inhibited initially and later recovered to some extent. Under the stress of low concentrations of AgNPs, the soluble protein content in the leaves of Typha orientalis increases significantly, but decreases more significantly with increasing concentrations of AgNPs. Low concentrations of AgNPs promote chlorophyll synthesis in the leaves of Typha orientalis , but the chlorophyll content subsequently falls to pre-stress levels. In contrast, high concentrations of AgNPs cause a certain inhibition to generate chlorophyll. Meanwhile, the results show that the silver concentrations of plant tissues increase with the exposure of concentrations of AgNPs and they have a positive relationship with the exposure of concentrations of AgNPs.展开更多
AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis ...AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis in rats.METHODS: TO at 10 mg/kg was orally administered, followed by 75 μg/kg CCK octapeptide injected subcutaneously three times after 1, 3 and 5 h. This whole procedure was repeated for 5 d. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60 and HSP72, and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in typical laboratory and morphological changes of experimentally-induced pancreatitis.RESULTS: TO significantly decreased the pancreatic weight/body weight ratio in CCK octapeptide-induced acute pancreatitis. TO also increased the pancreatic levels of HSP60 and HSP72. Additionally, the secretion of IL-6 and TNF-α decreased in the animals treated with TO.CONCLUSION: TO may have a protective effect against CCK octapeptide-induced acute pancreatitis.展开更多
In this study,the fifth-generation Pennsylvania State University-National Center for Atmospheric Research (PSU-NCAR) Mesoscale Model (MM5) is used to simulate Typhoon Mindulle (2004) at high resolution (3-km grid size...In this study,the fifth-generation Pennsylvania State University-National Center for Atmospheric Research (PSU-NCAR) Mesoscale Model (MM5) is used to simulate Typhoon Mindulle (2004) at high resolution (3-km grid size).The data from measurements show that in the upper atmosphere the existence of an upper jet is important to the transition cyclone.When Mindulle moved to the area of the upper jet entrance,where high-altitude divergence existed, the pumping of the high-altitude divergence would enhance the vertical motion and low-level cyclone convergence. The enhanced vertical motion was confirmed by the simulation results and indicated that the existence of upper divergence enhanced the vertical motion which was favorable for the maintenance of Typhoon Mindulle.The process of extratropical transition (ET) and re-intensification always accompanies the process of cold air invasion. This process enhances the baroclinicity of the atmosphere and the formation of front at high altitudes, which converts baroclinic potential energy into kinetic energy and strengthens the cyclone vortex.The distributions of equivalent potential temperature (θe) and temperature anomalies show that the warm-core of the typhoon at the tropopause aids the re-intensification of the system. As the typhoon reenters the ocean, latent heat flux (LHF) increases in the north and west and the strong reflectivity and vertical motion occur in the east and southeast,and the west.With the re-intensification of the typhoon the wind field evolves from an oval to a circle at the lower atmosphere, the area coverage by high winds increases, and the distribution of the tangential wind shows an asymmetric pattern.展开更多
Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-...Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-caryophyllene （3.25%）, eugenol acetate （23%）, cis-13-docosenamide （3.2 1%）, presenting more than 96% of the oil. This oil was examined as a fumigant and repellent agent against adults of the red flour beetle, Tribolium castaneum. Data showed that various concentrations of the oil have revealed dramatic repellent activity against the tested insect, where it gave 100% repellency by 1.0, 0.8, and 0.2% clove oil at 4, 8, 10 min, respectively. The RTs0 and RT95 were 1.1 and 8.0 min at 0.2% and 0.4, 2.6 min at 0.8%, respectively. In addition, fumigation assay has also exhibited strong fumigant activity toward the adults of T. castaneum. At 100 μL oil/L air, mortality was 75, 80 and 100% after 6, 7, and 8 days exposure period with LCs0 and LC95 17 and 70 μL/L air, respectively. The utilization of clove oil for its potential effects against stored product insect is discussed.展开更多
Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in ...Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in according with the requirements of high water head pumped storage hydro-plant should be 1.2 times of the water head special high-pressure packer permeability test compared with normal to test the permeability of rock and rock cleavage pressure value. The test results on the choice of design options often play a decisive role. Based on the engineering practice, the authors studied the drillhole high-pressure packer permeability test in the pumped storage hydro-plant's underground powerhouse, by the analysis of test results, this article offers a demonstration of the deformation of rock fracture witch under building in the condition of high-pressure water head, it provides a more detailed engineering geological background.展开更多
文摘DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.
基金Supported by the Approval Project of Guiyang University of Traditional Chinese Medicine(GTCM Nei[2014]24)
文摘[Objective] To study the preparation of Zipu Capsule and its clinical effect on the treatment of chronic periodontitis. [Method] The extraction, concentration, drying and preparation process of the raw herbal materials for Zipu Capsule were selected out. And 60 cases of chronic periodontitis patients were randomly assigned to two groups and were treated with conventional therapy and nursing care, while the treatment group was added with taking the Zipu capsule, to compare the cura- tive effects of the two groups. [Result] Both of two groups showed decreases in gingival index （GO, periodontal pocket depth （PD） and attachment loss values （AL） after treatment, and those of the treatment group was significantly lower than those of the control group （P〈0.05）. The total curative effective rate of the treatment group was 91.17%, and 76.92% in the control group, and the differences between the two groups reached the significant level （P〈0.05）. [Conclusion] Zipu Capsule had definite therapeutic effect in treatment of chronic periodontitis and less adverse re- actions, which laid the foundation for the further development into drug products.
基金Supported by the State key Subjecet of Botany at Hainan University (071001)the Malor Scientific Research Protect of Hainan Province, China(20080137)~~
文摘[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.
基金Project(21465016)supported by the National Natural Foundation of China
文摘This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.
基金supported by the National Natural Science Foundation of China (No.40672101)the Key National Natural Science Foundation of China (No.40730422)+1 种基金the National Science and Technology Key Special Project from the Ministry of Technology of China (No. 2008ZX05034)the China Scholarship Council (CSC)
文摘To evaluate the effect of tectonic deformation on coal reservoir properties, we provide an analysis of the types of tectonically deformed coal, macroand microscopic deformation and discuss pore structural characteristics and connectivity based on samples from the Puhe and Shanchahe coal mines. Our research shows that the tectonically deformed coal mostly includes cataclastic structural coal, mortar structural coal and schistose structural coal of a brittle deformation series. The major pore structures of different types of tectonically deformed coal are transitional pores and micropores. The pore volumes of macropores and visible fracture pores produced by structural deformations vary over a large range and increase with the intensity of tectonic deformation. Mesopores as connecting passages develop well in schistose structural coal. According to the shapes of intrusive mercury curves, tectonically deformed coal can be divided into parallel, open and occluded types. The parallel type has poor connectivity and is relatively closed; the open type reflects uniformly developed open pores with good connectivity while the occluded type is good for coalbed methane enrichment, but has poor connectivity between pores.
基金The National Natural Science Foundation of China(No.51479034,5151101102)
文摘To probe the influence and the adverse-resistance characteristics of wetland plants in presence of silver nanoparticles (AgNPs), the changes in the physiological and biochemical characteristics (including the superoxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity, soluble protein content, and chlorophyll content) of Typha orientalis exposed to different concentrations of AgNPs solutions (0, 0.1, 1, 20 and 40 mg/L) were explored. Meantime, the accumulation of silver content in these plants was revealed. The results show that under low concentrations of AgNPs, the SOD and POD activities in the leaves of Typha orientalis are strengthened to different degrees. However, high concentrations of AgNPs inhibit the activities of SOD and POD. Under the stress of different concentrations of AgNPs, the CAT activities are inhibited initially and later recovered to some extent. Under the stress of low concentrations of AgNPs, the soluble protein content in the leaves of Typha orientalis increases significantly, but decreases more significantly with increasing concentrations of AgNPs. Low concentrations of AgNPs promote chlorophyll synthesis in the leaves of Typha orientalis , but the chlorophyll content subsequently falls to pre-stress levels. In contrast, high concentrations of AgNPs cause a certain inhibition to generate chlorophyll. Meanwhile, the results show that the silver concentrations of plant tissues increase with the exposure of concentrations of AgNPs and they have a positive relationship with the exposure of concentrations of AgNPs.
文摘AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis in rats.METHODS: TO at 10 mg/kg was orally administered, followed by 75 μg/kg CCK octapeptide injected subcutaneously three times after 1, 3 and 5 h. This whole procedure was repeated for 5 d. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60 and HSP72, and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in typical laboratory and morphological changes of experimentally-induced pancreatitis.RESULTS: TO significantly decreased the pancreatic weight/body weight ratio in CCK octapeptide-induced acute pancreatitis. TO also increased the pancreatic levels of HSP60 and HSP72. Additionally, the secretion of IL-6 and TNF-α decreased in the animals treated with TO.CONCLUSION: TO may have a protective effect against CCK octapeptide-induced acute pancreatitis.
基金supported by the National Natural Science Foundation of China(Nos.40876004,40890155 and 40675060)National 973 Project(Nos.2009CB-421500 and 2007CB411801)
文摘In this study,the fifth-generation Pennsylvania State University-National Center for Atmospheric Research (PSU-NCAR) Mesoscale Model (MM5) is used to simulate Typhoon Mindulle (2004) at high resolution (3-km grid size).The data from measurements show that in the upper atmosphere the existence of an upper jet is important to the transition cyclone.When Mindulle moved to the area of the upper jet entrance,where high-altitude divergence existed, the pumping of the high-altitude divergence would enhance the vertical motion and low-level cyclone convergence. The enhanced vertical motion was confirmed by the simulation results and indicated that the existence of upper divergence enhanced the vertical motion which was favorable for the maintenance of Typhoon Mindulle.The process of extratropical transition (ET) and re-intensification always accompanies the process of cold air invasion. This process enhances the baroclinicity of the atmosphere and the formation of front at high altitudes, which converts baroclinic potential energy into kinetic energy and strengthens the cyclone vortex.The distributions of equivalent potential temperature (θe) and temperature anomalies show that the warm-core of the typhoon at the tropopause aids the re-intensification of the system. As the typhoon reenters the ocean, latent heat flux (LHF) increases in the north and west and the strong reflectivity and vertical motion occur in the east and southeast,and the west.With the re-intensification of the typhoon the wind field evolves from an oval to a circle at the lower atmosphere, the area coverage by high winds increases, and the distribution of the tangential wind shows an asymmetric pattern.
文摘Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-caryophyllene （3.25%）, eugenol acetate （23%）, cis-13-docosenamide （3.2 1%）, presenting more than 96% of the oil. This oil was examined as a fumigant and repellent agent against adults of the red flour beetle, Tribolium castaneum. Data showed that various concentrations of the oil have revealed dramatic repellent activity against the tested insect, where it gave 100% repellency by 1.0, 0.8, and 0.2% clove oil at 4, 8, 10 min, respectively. The RTs0 and RT95 were 1.1 and 8.0 min at 0.2% and 0.4, 2.6 min at 0.8%, respectively. In addition, fumigation assay has also exhibited strong fumigant activity toward the adults of T. castaneum. At 100 μL oil/L air, mortality was 75, 80 and 100% after 6, 7, and 8 days exposure period with LCs0 and LC95 17 and 70 μL/L air, respectively. The utilization of clove oil for its potential effects against stored product insect is discussed.
文摘Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in according with the requirements of high water head pumped storage hydro-plant should be 1.2 times of the water head special high-pressure packer permeability test compared with normal to test the permeability of rock and rock cleavage pressure value. The test results on the choice of design options often play a decisive role. Based on the engineering practice, the authors studied the drillhole high-pressure packer permeability test in the pumped storage hydro-plant's underground powerhouse, by the analysis of test results, this article offers a demonstration of the deformation of rock fracture witch under building in the condition of high-pressure water head, it provides a more detailed engineering geological background.
