Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-...Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-caryophyllene （3.25%）, eugenol acetate （23%）, cis-13-docosenamide （3.2 1%）, presenting more than 96% of the oil. This oil was examined as a fumigant and repellent agent against adults of the red flour beetle, Tribolium castaneum. Data showed that various concentrations of the oil have revealed dramatic repellent activity against the tested insect, where it gave 100% repellency by 1.0, 0.8, and 0.2% clove oil at 4, 8, 10 min, respectively. The RTs0 and RT95 were 1.1 and 8.0 min at 0.2% and 0.4, 2.6 min at 0.8%, respectively. In addition, fumigation assay has also exhibited strong fumigant activity toward the adults of T. castaneum. At 100 μL oil/L air, mortality was 75, 80 and 100% after 6, 7, and 8 days exposure period with LCs0 and LC95 17 and 70 μL/L air, respectively. The utilization of clove oil for its potential effects against stored product insect is discussed.展开更多
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites （especially flavanones, chalcone, and triterpenoids）. In our present study, three conventional solvent extraction （CSE） techn...Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites （especially flavanones, chalcone, and triterpenoids）. In our present study, three conventional solvent extraction （CSE） techniques and supercritical fluid extraction （SFE） techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques （maceration, soxhletion, and reflux）. An enriched extract of five secondary metabolites was collected using n-hexane：methanol （1：1） soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery （25.5%-84.9%） of selected secondary metabo- lites as compared to the CSE techniques （0.92%-66.00%）.展开更多
文摘Essential oil from clove buds Syzygium aromaticum L. Merr. ＆ Perry （Myrtaceae） was extracted using petroleum ether in Soxhlet apparatus. The resultant oil contained eugenol （48.92%）, caryophyllene （18.55%）, α-caryophyllene （3.25%）, eugenol acetate （23%）, cis-13-docosenamide （3.2 1%）, presenting more than 96% of the oil. This oil was examined as a fumigant and repellent agent against adults of the red flour beetle, Tribolium castaneum. Data showed that various concentrations of the oil have revealed dramatic repellent activity against the tested insect, where it gave 100% repellency by 1.0, 0.8, and 0.2% clove oil at 4, 8, 10 min, respectively. The RTs0 and RT95 were 1.1 and 8.0 min at 0.2% and 0.4, 2.6 min at 0.8%, respectively. In addition, fumigation assay has also exhibited strong fumigant activity toward the adults of T. castaneum. At 100 μL oil/L air, mortality was 75, 80 and 100% after 6, 7, and 8 days exposure period with LCs0 and LC95 17 and 70 μL/L air, respectively. The utilization of clove oil for its potential effects against stored product insect is discussed.
文摘Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites （especially flavanones, chalcone, and triterpenoids）. In our present study, three conventional solvent extraction （CSE） techniques and supercritical fluid extraction （SFE） techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques （maceration, soxhletion, and reflux）. An enriched extract of five secondary metabolites was collected using n-hexane：methanol （1：1） soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery （25.5%-84.9%） of selected secondary metabo- lites as compared to the CSE techniques （0.92%-66.00%）.
