蛋白制剂中Tween80含量检测研究

添加聚山梨醇酯80(又名Tween80)或其它表面活性剂是防止蛋白质药物制剂中蛋白聚体形成的主要手段,需要根据研发蛋白制剂的特点选择适当的准确快速测定Tween80含量的方法。综述了近年来在蛋白制剂中检测Tween80含量的研究进展情况。介绍了多种定量蛋白制剂中Tween80的方法,并比较了各方法的优缺点。尤其对于含有高浓度蛋白质的制剂样品中Tween80的定量测定,分析了不同的文献方法以及专利方法的可行性。

Primary Comments on Chemotaxonomy of Paris spp. Based on Saponins Analysis

Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.

HPLC-ELSD法测定食品中γ-氨基丁酸含量

建立了食品中γ-氨基丁酸的液相色谱蒸发光散射检测仪测定方法。该方法用水或10%甲醇提取样品,正己烷去油脂净化,然后用液相色谱/蒸发光散射检测仪测定。γ-氨基丁酸的浓度在0.01～0.5 mg/mL范围内与色谱峰面积呈良好的线性关系,相关系数r=0.9998。食品中γ-氨基丁酸的检出限为0.01 g/kg,加标回收率在90%以上。该方法快速、灵敏、准确。