省档案馆名人档案征集工作取得新进展

为进一步丰富省档案馆馆藏名人档案资料,省档案馆征集整理处加大对名人档案征集工作力度。近期,征集到省社会科学研究院研究员蓝华增、王国祥、郭大烈,云南大学博士生导师尹绍亭4位著名学者的声像、照片、证书、往来书信、手稿、学术成果等档案资料250余件。蓝华增,研究员,出生于1930年,四川成都人。1958年毕业于云南大学历史系,毕业后在中学任教。

中国男篮关键词

累: 隔天一次的力量课,训练师不仅要求上量,而且要求任何动作都要做到位,所以,记者接触到国手,见面的第一句话就是“累”。 时差: 现在的上午9点半到11点半、下午5点到7点的训练时间与以往不同,上午的课有时候要拖到12点才结束,洗澡、吃完饭差不多都下午1点半了。

手术室护理配合

急腹症重型中毒性休克385例手术配合/蓝华/护士进修杂志.-1989,5(2).-31-32.1.

Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa

The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,China.Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC.Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis.Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length.Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80infectious units per cell,respectively.Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis revealed four major structural proteins.These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.

Effects of Microcystis aeruginosa on life history of water flea Daphnia magna

Cyanobacterial blooms in eutrophic freshwater systems are a worldwide problem, creating adverse effects for many aquatic organisms by producing toxic mierocystins and deteriorating water quality. In this study, microcystins （MCs） in Microcystis aeruginosa, and Daphnia magna exposed to M. aeruginosa, were analyzed by HPLC-MS, and the effects of M. aeruginosa on D. magna were investigated. When D. magna was exposed to M. aeruginosa for more than 2 h, Microcystin-LR （MC-LR） was detected. When exposed to 1.5× 10^6, 3× 10^6, 0.75× 10^7, and 1.5× 10^7 cell/mL of M. aeruginosa for 96 h, average survival of D. magna for treatments were 23.33%, 33.33%, 13.33%, 16.67%, respectively, which were significantly lower than the average 100% survival in the control group （P 〈 0.05）. The adverse effects ofM. aeruginosa on body length, time for the first brood, brood numbers, gross fecundity, lifespan, and population growth olD. magna were density-dependent. These results suggest that the occurrence of M. aeruginosa blooms could strongly inhibit the population growth of D. magna through depression of survival, individual growth and gross fecundity. In the most serious situations, M. aeruginosa blooms could undermine the food web by eliminating filter-feeding zooplankton, which would destroy the ecological balance of aquaculture water bodies.

Monitoring cyanobacteria-dominant algal blooms in eutrophicated Taihu Lake in China with synthetic aperture radar images

Monitoring algal blooms by optical remote sensing is limited by cloud cover.In this study,synthetic aperture radar(SAR) was deployed with the aim of monitoring cyanobacteria-dominant algal blooms in Taihu Lake in cloudy weather.The study shows that dark regions in the SAR images caused by cyanobacterial blooms damped the microwave backscatter of the lake surface and were consistent with the regions of algal blooms in quasi-synchronous optical images,confirming the applicability of SAR for detection of surface blooms.Low backscatter may also be associated with other factors such as low wind speeds,resulting in interference when monitoring algal blooms using SAR data alone.After feature extraction and selection,the dark regions were classified by the support vector machine method with an overall accuracy of 67.74%.SAR can provide a reference point for monitoring cyanobacterial blooms in the lake,particularly when weather is not suitable for optical remote sensing.Multi-polarization and multi-band SAR can be considered for use in the future to obtain more accurate information regarding algal blooms from SAR data.

The trophic state of lake water regulates spatial-temporal variations of bloom-forming Microcystis

Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundances of microcystin-producing Microcystis in a given bloom.In this study,we used a harmful algal bloom in Chaohu Lake,eastern China,as an example to investigate the mutual relationship between different nutrient states and environmental factors,and the impact on Microcystis.Overall,cyanobacteria and Microcystis were more abundant in the middle and western parts of the lake under high nutrients levels,while in the eastern part,nutrient concentrations were low enough to limit biomass,and their fluctuations affected the contents of toxic Microcystis.Moreover,microcystin concentration was correlated positively to nutrient levels and Microcystis biomass during bloom developing in 2013 from June to August.Temporally,the cellular content of total microcystin was lowest when the bloom peaked in intensity.Our results suggest that lake eutrophication not only results in cyanobacterial blooms,but may also increase the proportion of toxic Microcystis species and their cell-bound MCs contents(i.e.microcystin cell quotas) under mild eutrophication.The present investigation provided molecular evidence for the selection of MC-producing and non-MC-producing genotypes.The current study provides new evidence advocating the monitoring of partitions of large lakes when studying cyanobacteria and toxin-contaminated freshwaters,which will be beneficial for both water agencies and water researchers.

Numerical simulation of an algal bloom in Dianshan Lake

A hydrodynamic model and an aquatic ecology model of Dianshan Lake,Shanghai,were built using a hydrodynamic simulation module and the water quality simulation module of Delft3D,which is an integrated modelling suite offered by Deltares. The simulated water elevation,current velocity,and direction were validated with observed data to ensure the reliability of hydrodynamic model. The seasonal growth of different algae was analyzed with consideration of observed and historical data,as well as simulated results. In 2008,the dominant algae in Dianshan Lake was Bacillariophyta from February to March,while it was Chlorophyta from April to May,and Cyanophyta from July to August. In summer,the biomass of Cyanophyta grew quickly,reaching levels much higher than the peaks of Bacillariophyta and Chlorophyta. Algae blooms primarily occurred in the stagnation regions. This phenomenon indicates that water residence time can influence algal growth significantly. A longer water residence time was associated with higher algal growth. Two conclusions were drawn from several simulations: reducing the nutrients inflow had little effect on algal blooms in Dianshan Lake; however,increasing the discharge into Dianshan Lake could change the flow field characteristic and narrow the range of stagnation regions,resulting in inhibition of algal aggregation and propagation and a subsequent reduction in areas of high concentration algae.

The decline process and major pathways of Microcystis bloom in Taihu Lake, China

Eutrophication has become a serious concern in many lakes, resulting in cyanobacterial blooms. However, the mechanism and pathways of cyanobacteria decline are less understood. To identify and define the growth and decline of Microcystis blooms in Taihu Lake of China, and to illuminate the destination of surface floating blooms, we investigated the biomass distribution and variations in colony size, morphology, and floating velocity from October 2008 to September 2009. The results showed that the Microcystis bloom declined in response to biomass decrease, colony disaggregation, buoyancy reduction, and increased phytoplankton biodiversity, and these indicative parameters could be applied for recognition of the development phases of the bloom. Three major decline pathways were proposed to describe the bloom decline process, colony disaggregation （Pathway I）, colony settlement （Pathway II）, and cell lysis in colonies （Pathway III）. We proposed a strategy to define the occurrence and decline of Microcystis blooms, to evaluate the survival state under different stress conditions, and to indicate the efficiency of controlling countermeasures against algal blooms.

Changes in phytoplankton communities along nutrient gradients in Lake Taihu:evidence for nutrient reduction strategies

An annual investigation on phytoplankton communities was conducted to reveal the effects of nutrients on phytoplankton assemblages in Lake Taihu,East China. A total of 78 phytoplankton taxa were identified. Phytoplankton biomass was higher in the northern part of the lake than in the southern part. Cyanobacteria and Bacillariophyta alternated dominance in the northern area,where algal blooms often appear,and co-dominated in the southern area. In the northern part,the proportions of cyanobacteria and Bacillariophyta varied significantly in total biovolume,both along the phosphorus(P) gradient,and between total nitrogen levels(≤3 mg/L and >3 mg/L TN). The proportions of cyanobacteria and Bacillariophyta had no signif icant variations in total biovolume along P and N(nitrogen) gradients in the southern part. Correlation analysis and CCA results revealed that P was the key factor regulating phytoplankton community structure. Nitrogen was also important for the phytoplankton distribution pattern. It was concluded that nutrient structure was heterogeneous in space and shaped the distribution pattern of phytoplankton in the lake. Both exogenous P and internally sourced Prelease needs to be considered. N reduction should be considered simultaneously with P control to efficiently reduce eutrophication and algal blooms.

Mineralization and Availability of Azolla and Cyanobacteria Biomass Nutrients in Rice Soil

Two sets of experiments were conducted to study the chemical composition, mineralization and availability of nutrients （particularly N） of Azolla biomass and cyanohacteria blooms in submerged rice soil. One set of experiment was conducted with Azolla without soil and the other with both Azolla and cyaobacteria with soil, in laboratory condition. Large samples were collected from the rice fields. The study shows that Azolla and cyanobacterial biomass contain good amounts of C, N, P and Ca. The chemical analysis of oxidizable C, total N, P and Ca showed that Azolla biomass contain 20.7% oxidizable C, which is higher than the cyanobacterial biomass （9.18%）. The N content in Azolla biomass was found to be 4.32%, than that of cyanobacterial biomass, i.e., 2.57%, however, P and Ca contents were also found to be higher in Azolla averaging to 124.83 ppm and 345.3 mg/100 g, respectively than cyanobacteria. The leachate analysis revealed that Azolla biomass release about 39.18% to 64.48% oxidizable C and about 19.23% to 33% N after 45 days of incorporation of soil dilution. Incorporation of Azolla and cyanobacterial biomass N@ 100 kg/ha significantly improved the oxidizable organic C, total as well as available N content in soil up to 75 days of incubation. Due to the incorporation of Azolla in soil, oxidizable organic C increased 25.51%, total N 4.10% and available N 47.65%. Almost similar trend of increase was also observed with the incorporation of cyanobacterial blooms.

Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms

Silver carp is one of the most important planktivorous fish in Chinese aquaculture and plays a significant role controlling cyanobacterial blooms. A balanced gut microbiota is crucial for growth and health of the host because of its important roles in immune defense, digestion of complex carbohydrates, and production of enterocytes. In our study, the dominant bacterial and cellulolytic bacterial （Clostridium 1, Clostridium 111, Clostridium XIVab, and Fibrobacter） communities in the contents and mucus of the silver carp gut （foregut, midgut, and hindgut） were analyzed by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction （qPCR） analyses. The results revealed that the dominant and cellulolytic bacterial communities were significantly different among gut regions as well as in contents and mucus. Bacterial diversity and richness in contents and mucus increased along the gut and were higher in contents than those in local mucus. A sequence analysis of gut samples exhibited the conservative phylotypes of Proteobacteria, Actinobacteria, and Firmicutes. The gut of silver carp harbored an abundance of cellulolytic bacteria, particularly Clostridium XIVab. The foregut segment had the highest proportions of the four cellulolytic bacteria, followed by the midgut and hindgut. However, the proportions of cellulolytic species in the silver carp gut was much lower than those in the terrestrial vertebrate gastrointestinal tract. We conclude that gut bacteria could help silver carp obtain energy from cyanobacteria, which may be why silver carp can maintain high growth rates during cyanobacterial blooms.

Effect of cyanobacteria extract on some associated enzymes in mouse liver in vivo

Blooms of toxin-producing cyanobacteria have become increasingly common in the surface water of the world. In this study, we studied the dose- and time-dependent effects of a microcystin （MC） extract of cyanobacteria from Dianchi Lake in China on liver weight/body weight ratio and superoxide dismutase （SOD）, lactate dehydrogenase （LDH） and glutathione peroxidase （GSH-Px） activities in mouse liver. We found that exposure to the cyanobacterial extract （CE） resulted in increase in liver weight/body weight ratio in a dose-dependent manner, and the mouse liver reached the maximum size at 1 h post-exposure （pe）. SOD activity in mouse liver decreased in a dose-dependent manner, and time course study indicated that it decreased significantly at 1 and 2 h pe, and resumed at 3 h pc as compared to control. CE caused LDH activity in the livers of mice to decrease in a dose- and time-dependent maimer except a small increase in 30 min pe mice. GSH-Px activity increased in a dose-dependent manner, and was higher than that in the control over the 3 h observation period. The present findings suggest that oxidative damage may be involved in the toxicity of microcystins on mouse.

Effect of Iron on Growth, Pigmentation and Antioxidative Activity of Bloom Forming Cyanobacteria

Cyanobacterial blooms are ubiquitous in fresh and brackish eutrophic waters in India. The cyanotoxins produced by many bloom forming cyanobacteria severely affect the health of animals, fishes, birds and human beings. Different physical and chemical factors contribute towards bloom formation. Ten bloom forming cyanobacteria were isolated from natural blooms of northern India. The strains were purified and enriched in the laboratory. The aim of this study was to understand the influence of iron on growth, pigmentation and antioxidative activity of enzymes-catalase and ascorbate peroxidase of bloom forming cyanobacteria. Results show that different strains of bloom forming cyanobacteria attain optimum growth at varied concentration of iron. The cyanobacterial strains like Synechocystis aquatalis, Merismopedia glauca, Anabaena variabilis and Anabaena iyengarii exhibit maximum growth at low iron concentration （2 pM） while some species require higher concentration of iron for their optimum growth namely, Arthrospira platensis show optimum growth at 10uM, and Nostocpaludosum shows maximum growth at 100uM concentration of iron. It was also noticed that chlorophyll and phycobiliprotein content also varies with change in iron concentration. The activity of antioxidative enzymescatalase and ascorbate peroxidase was noticed in all ten cyanobacterial strains. In the light of the findings, it seems that Arthrospira platensis possess maximum catalase and ascorbate peroxidase activity. Increment in concentration beyond optimum value leads to deterioration in the growth, pigment content and enzymatic activity of the cyanobacterial strains. Knowledge about the factors influencing growth of bloom forming cyanobacteria will help to work out ways for eradication of hazardous cyanobacterial blooms.

《“鸡爪出笼”》

星星姐姐:《抢鸡爪》《“鸡爪出笼”》《嘴馋之“恶果”》《有趣的鸡爪之争》,读完全文,请你从这几个题目中选一个你认为最恰当的题目填在标题处的横线上。

An eco-environmental friendly operation: An effective method to mitigate the harmful blooms in the tributary bays of Three Gorges Reservoir

Harmful algal blooms, which frequently occur in different tributaries, have become a serious water quality problem of the Three Gorges Reservoir (TGR) since its initial filling. A three-year monitoring campaign from 2008 to 2010 was conducted to identify the controlling factors of algal blooms and effective preventive measures. The following results were obtained: 1) Layered and bidirectional currents, called intrusive density currents, dominate the tributary bays of the TGR year round; 2) continuously increasing water level can make the intruding density current shift from the middle to the surface, and surface intrusive water can reduce the surface water temperature and break the stable stratification of the tributary; 3) the appearing or fading away of algal blooms is closely associated with fluctuations in the TGR water level. Thus, the water level fluctuation patterns of different "tide-types" may prevent the occurrence of algal blooms in the spring and summer, and the "stair-type" tides may control autumn blooms during the impounding period. These methods were feasible and effective throughout the actual TGR's operation in 2009 2011. These patterns formed an eco-environmentally friendly operation (EEFO) to mitigate algal blooms. As an in situ treatment with easier operation and less negative impact and loss, compared with chemical and filter-feeding fish methods, the EEFO is currently considered as the most feasible remedial measure to remit the eco-environmental problems at the Three Gorges Dam (TGD).

Black band disease as a possible factor of the coral decline at the northern reef-flat of Yongxing Island,South China Sea

Black band disease （BBD）, characterized by the Cyanobacterial dominated pathogenic consortium, is thought to play a key role in the global decline of the coral reef ecosystems. The present paper originally documents a case of BBD from Yongxing Island （Xisha Islands, South China Sea）, and further probes the reasons of this abnormal phenomenon. Prior to 2007, corals at northern reef-flat of Yongxing Isand were in healthy growth. Catastrophic coral mortality occurred between 2007 and 2008. The 16S rRNA gene sequencing and PCR amplification, with universally conserved primers, were applied to detect the conta- gious bacterial community of the microbial mat. The results demonstrated that six bacterial divisions constituted the clone libraries derived from the BBD mat, and that Cyanobacteria are the most diversely represented group that inhabit BBD bacteri- al mats, despite the fact that species in five others divisions （a-Proteobacteria, y-Proteobacteria, Bacteroidetes, Verrucomi- crobia and Actinobacteria） are also consistently diverse within the BBD mats of diseased coral. Other factors such as coral bleaching, typhoons, ocean acidification and crown-of-thorns starfish outbreaks, are not primarily responsible for the coral mortality within such a short time interval. The disaster expansion of BBD associated with Cyanobacterial blooms is a more likely mechanism impacting these coral reefs. Excessive human activity enhances the eutrophication of the marine water of the reefal region and may result in occurrence of the BBD.