作为上海西童公学的奠基人,乔治·蓝宁在任校长期间,为在校学生及汉语初学者编写教材《Easy Steps in Chinese Studies》。在编写教材时,蓝宁以"简易"为原则,试图通过所设计的"步骤"——重模仿、避语法、强词汇...作为上海西童公学的奠基人,乔治·蓝宁在任校长期间,为在校学生及汉语初学者编写教材《Easy Steps in Chinese Studies》。在编写教材时,蓝宁以"简易"为原则,试图通过所设计的"步骤"——重模仿、避语法、强词汇,让汉语学习变得可操作且有抓手;在教学法上,蓝宁要求学习者要充分发挥主观能动,自主选择内容,以提高学习效率;教材能基于中国国情,不断加强对中国文化与生活的渗透,已具备成熟汉语教材的基本特质,但由于历史条件的局限性,教材仍存在学情把握不准、字典化倾向严重等问题。展开更多
[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identificati...[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identification of hybrid rape-seeds was established with SSR molecular markers; meanwhile, the test seeds were planted in the field for comparison and verification. [Result] A method for rapid identification of Ningza 11 seeds purity with SSR molecular markers was estab-lished： DNA from seeds germinated in the night was extracted by alkaline lysis method; the PCR amplification was performed for 2 h, and electrophoresis for 1.5 h, and a silver staining for 10 minutes. It took less than one day to from obtaining sampling seeds to obtaining the purity identification result, so a skil ed professional can complete the detection of at least 6 ×96 = 576 seeds per weekday. By using this set of detection system, the measured purity of seeds from nine samples was extremely significantly positively correlated to the actual purity identified in the field test, with a correlation coefficient of up to 0.984 （P〈0.01）. [Conclusion] This SSR-PCR molecular identification system can be applied for rapid and accurate identifi-cation of Ningza 11 hybrid seeds.展开更多
文摘作为上海西童公学的奠基人,乔治·蓝宁在任校长期间,为在校学生及汉语初学者编写教材《Easy Steps in Chinese Studies》。在编写教材时,蓝宁以"简易"为原则,试图通过所设计的"步骤"——重模仿、避语法、强词汇,让汉语学习变得可操作且有抓手;在教学法上,蓝宁要求学习者要充分发挥主观能动,自主选择内容,以提高学习效率;教材能基于中国国情,不断加强对中国文化与生活的渗透,已具备成熟汉语教材的基本特质,但由于历史条件的局限性,教材仍存在学情把握不准、字典化倾向严重等问题。
基金Supported by the Jiangsu Provincial Agricultural Science and Technology Innovation Fund[CX(11)1026]National Science and Technology Support Program(2010BAD01B-10)863 Major Project(2011AA10A10403)~~
文摘[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identification of hybrid rape-seeds was established with SSR molecular markers; meanwhile, the test seeds were planted in the field for comparison and verification. [Result] A method for rapid identification of Ningza 11 seeds purity with SSR molecular markers was estab-lished： DNA from seeds germinated in the night was extracted by alkaline lysis method; the PCR amplification was performed for 2 h, and electrophoresis for 1.5 h, and a silver staining for 10 minutes. It took less than one day to from obtaining sampling seeds to obtaining the purity identification result, so a skil ed professional can complete the detection of at least 6 ×96 = 576 seeds per weekday. By using this set of detection system, the measured purity of seeds from nine samples was extremely significantly positively correlated to the actual purity identified in the field test, with a correlation coefficient of up to 0.984 （P〈0.01）. [Conclusion] This SSR-PCR molecular identification system can be applied for rapid and accurate identifi-cation of Ningza 11 hybrid seeds.
