The possibility of cultivation of lndigofera tinctoria L. plants in ecologically degraded and saline lands of the Aral Sea Basin was studied. A new salt-resistant Indigofera plant variety ＂Feruz-l＂ was producing by ...The possibility of cultivation of lndigofera tinctoria L. plants in ecologically degraded and saline lands of the Aral Sea Basin was studied. A new salt-resistant Indigofera plant variety ＂Feruz-l＂ was producing by the classic methods of genetics and modem biotechnology.Agro-technology of cultivation as main and secondary culture after wheat was developed and recommended to the farmers of the country.The biotechnology of natural plant pigment indigo extraction has been developed and was recommended for using in different fields of industry： textile, pharmacy-pharmacology, perfume-cosmetic, architectural-decorative and food industry. Quantitative and qualitative HPLC （high pressure liquid chromatography） method of determination of plant natural indigo has been also developed.展开更多
Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (S...Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (Spinacia oleracea L.) and rape(Brassica campestrisL.) to study the effects of N forms and N rates on their growth and nitrate accumulation. Theresults indicated that application of ammonium chloride, ammonium nitrate, sodium nitrate and ureasignificantly increased the yields and nitrate concentrations of Peking cabbage and spinach.Although no significant difference was found in the yields after application of the 4 N forms,nitrate N increased nitrate accumulation in vegetables much more than ammonium N. The vegetableyields were not increased continuously with N rate increase, and oversupply of N reduced the plantgrowth, leading to a yield decline. This trend was also true for nitrate concentrations in somevegetables and at some sampling times. However, as a whole, the nitrate concentrations in vegetableswere positively correlated with N rates. Thus, addition of N fertilizer to soil was the major causefor increases in nitrate concentrations in vegetables. Nitrate concentrations were much higher inroots, stems and petioles than in blades at any N rate.展开更多
AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Spragu...AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxi-bustion group, moxibustion plus disodium cromoglycate (M + DC) group and moxibustion plus normal saline (M+ NS) group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M + DC and M + NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxy- lin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas. RESULTS: An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group (45.91 ± 11.41 vs 32.58 ± 8.28, P < 0.05). After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed. CONCLUSION: Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint.展开更多
The main aim of this work was to investigate the effects of salt stress conditions on the inorganic and organic compounds of extracts from six filamentous cyanobacteria： Arthospira platensis, Oscillatoria sp., Oscill...The main aim of this work was to investigate the effects of salt stress conditions on the inorganic and organic compounds of extracts from six filamentous cyanobacteria： Arthospira platensis, Oscillatoria sp., Oscillatoria salina, Tolypothrix sp., Oscillatoria sp. SWU （Srinakharinwirot University）121 and Tolypothrix sp. SWU213. All cyanobacteria were cultures in BG （blue-green algae）11 medium： pH 7.5 at 35 ℃ for 30 days of sodium chloride in the culture medium presented 0-1.0 M. The cyanobacteria isolates grew well in BGH medium, nevertheless, growth of the majority of isolates was reduced by about 50% in the same medium containing 0.5 M NaCl. The inorganic compounds such as, Na^＋, K^＋, Ca^2＋, Mg^2＋, NH4^＋ and NO3^- were determined. All ions were reduced when NaCI was increased and Na^＋ showed highest amount in the medium followed with Mg^2＋, NH4^＋, NO3^- and Ca^2＋ in all cyanobaeteria. The organic compounds such as, betaine, proline and total lipid were determined under normal and salt stress conditions. We found that all cyanobacteria increased interesting organic compound under salt stress condition at least two folds to compare with normal condition. This is the first finding indicated that freshwater filamentous cyanobacteria could grow under salt stresses by accumulation of some organic compounds as osmoprotectants such as betaine and amino acids, being the reduction related to the amount of inorganies compounds present in cultures.展开更多
Photodynamic therapy plays an important role in cancer treatment.In this work,methylene blue(MB)-embedded calcium carbonate nanorods(CaCO_3-MB NRs)have been synthesized for p H-responsive photodynamic therapy and ultr...Photodynamic therapy plays an important role in cancer treatment.In this work,methylene blue(MB)-embedded calcium carbonate nanorods(CaCO_3-MB NRs)have been synthesized for p H-responsive photodynamic therapy and ultrasound imaging.The morphology of CaCO_3-MB NRs can be controlled by modulating the concentration of Na_2CO_3 aqueous solution.The generation of effective reactive oxygen species(ROS)were confirmed by 1,3-diphenylisobenzofuran(DPBF)probe.Both photodynamic therapy performance and echogenic performance of CaCO_3-MB NRs were investigated to confirm the feasibility of CaCO_3-MB nanohybrids for ultrasound image-guided photodynamic therapy.展开更多
文摘The possibility of cultivation of lndigofera tinctoria L. plants in ecologically degraded and saline lands of the Aral Sea Basin was studied. A new salt-resistant Indigofera plant variety ＂Feruz-l＂ was producing by the classic methods of genetics and modem biotechnology.Agro-technology of cultivation as main and secondary culture after wheat was developed and recommended to the farmers of the country.The biotechnology of natural plant pigment indigo extraction has been developed and was recommended for using in different fields of industry： textile, pharmacy-pharmacology, perfume-cosmetic, architectural-decorative and food industry. Quantitative and qualitative HPLC （high pressure liquid chromatography） method of determination of plant natural indigo has been also developed.
基金Project supported by the National Key Basic Research Support Foundation (No. G1999011707)the National Natural Science Foundation of China (Nos. 39970429, 49890330, 30070429 and 40201028).
文摘Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (Spinacia oleracea L.) and rape(Brassica campestrisL.) to study the effects of N forms and N rates on their growth and nitrate accumulation. Theresults indicated that application of ammonium chloride, ammonium nitrate, sodium nitrate and ureasignificantly increased the yields and nitrate concentrations of Peking cabbage and spinach.Although no significant difference was found in the yields after application of the 4 N forms,nitrate N increased nitrate accumulation in vegetables much more than ammonium N. The vegetableyields were not increased continuously with N rate increase, and oversupply of N reduced the plantgrowth, leading to a yield decline. This trend was also true for nitrate concentrations in somevegetables and at some sampling times. However, as a whole, the nitrate concentrations in vegetableswere positively correlated with N rates. Thus, addition of N fertilizer to soil was the major causefor increases in nitrate concentrations in vegetables. Nitrate concentrations were much higher inroots, stems and petioles than in blades at any N rate.
基金Supported by National Basic Research Program of China (973 program), No. 2009CB522900National Natural Science Foundation of China, No. 30973785+1 种基金Shanghai Leading Academic Discipline Project, No. S30304Shanghai Rising-Star Program, No. 10QA1406100
文摘AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxi-bustion group, moxibustion plus disodium cromoglycate (M + DC) group and moxibustion plus normal saline (M+ NS) group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M + DC and M + NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxy- lin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas. RESULTS: An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group (45.91 ± 11.41 vs 32.58 ± 8.28, P < 0.05). After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed. CONCLUSION: Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint.
文摘The main aim of this work was to investigate the effects of salt stress conditions on the inorganic and organic compounds of extracts from six filamentous cyanobacteria： Arthospira platensis, Oscillatoria sp., Oscillatoria salina, Tolypothrix sp., Oscillatoria sp. SWU （Srinakharinwirot University）121 and Tolypothrix sp. SWU213. All cyanobacteria were cultures in BG （blue-green algae）11 medium： pH 7.5 at 35 ℃ for 30 days of sodium chloride in the culture medium presented 0-1.0 M. The cyanobacteria isolates grew well in BGH medium, nevertheless, growth of the majority of isolates was reduced by about 50% in the same medium containing 0.5 M NaCl. The inorganic compounds such as, Na^＋, K^＋, Ca^2＋, Mg^2＋, NH4^＋ and NO3^- were determined. All ions were reduced when NaCI was increased and Na^＋ showed highest amount in the medium followed with Mg^2＋, NH4^＋, NO3^- and Ca^2＋ in all cyanobaeteria. The organic compounds such as, betaine, proline and total lipid were determined under normal and salt stress conditions. We found that all cyanobacteria increased interesting organic compound under salt stress condition at least two folds to compare with normal condition. This is the first finding indicated that freshwater filamentous cyanobacteria could grow under salt stresses by accumulation of some organic compounds as osmoprotectants such as betaine and amino acids, being the reduction related to the amount of inorganies compounds present in cultures.
基金supported by the National Key Research and Development Program of China (2017YFA0106100, 2016YFA0201501)National Natural Science Foundation of China (51325304, 51373017, 51473014, 51521062, 51773013)+1 种基金the Fundamental Research Funds for the Central Universities (BHYC1705A)Higher Education and High-quality and World-class Universities (PY201603)
文摘Photodynamic therapy plays an important role in cancer treatment.In this work,methylene blue(MB)-embedded calcium carbonate nanorods(CaCO_3-MB NRs)have been synthesized for p H-responsive photodynamic therapy and ultrasound imaging.The morphology of CaCO_3-MB NRs can be controlled by modulating the concentration of Na_2CO_3 aqueous solution.The generation of effective reactive oxygen species(ROS)were confirmed by 1,3-diphenylisobenzofuran(DPBF)probe.Both photodynamic therapy performance and echogenic performance of CaCO_3-MB NRs were investigated to confirm the feasibility of CaCO_3-MB nanohybrids for ultrasound image-guided photodynamic therapy.
