The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,China.Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC...The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,China.Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC.Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis.Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length.Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80infectious units per cell,respectively.Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis revealed four major structural proteins.These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.展开更多
Cyanobacterial blooms in eutrophic freshwater systems are a worldwide problem, creating adverse effects for many aquatic organisms by producing toxic mierocystins and deteriorating water quality. In this study, microc...Cyanobacterial blooms in eutrophic freshwater systems are a worldwide problem, creating adverse effects for many aquatic organisms by producing toxic mierocystins and deteriorating water quality. In this study, microcystins （MCs） in Microcystis aeruginosa, and Daphnia magna exposed to M. aeruginosa, were analyzed by HPLC-MS, and the effects of M. aeruginosa on D. magna were investigated. When D. magna was exposed to M. aeruginosa for more than 2 h, Microcystin-LR （MC-LR） was detected. When exposed to 1.5× 10^6, 3× 10^6, 0.75× 10^7, and 1.5× 10^7 cell/mL of M. aeruginosa for 96 h, average survival of D. magna for treatments were 23.33%, 33.33%, 13.33%, 16.67%, respectively, which were significantly lower than the average 100% survival in the control group （P 〈 0.05）. The adverse effects ofM. aeruginosa on body length, time for the first brood, brood numbers, gross fecundity, lifespan, and population growth olD. magna were density-dependent. These results suggest that the occurrence of M. aeruginosa blooms could strongly inhibit the population growth of D. magna through depression of survival, individual growth and gross fecundity. In the most serious situations, M. aeruginosa blooms could undermine the food web by eliminating filter-feeding zooplankton, which would destroy the ecological balance of aquaculture water bodies.展开更多
Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundanc...Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundances of microcystin-producing Microcystis in a given bloom.In this study,we used a harmful algal bloom in Chaohu Lake,eastern China,as an example to investigate the mutual relationship between different nutrient states and environmental factors,and the impact on Microcystis.Overall,cyanobacteria and Microcystis were more abundant in the middle and western parts of the lake under high nutrients levels,while in the eastern part,nutrient concentrations were low enough to limit biomass,and their fluctuations affected the contents of toxic Microcystis.Moreover,microcystin concentration was correlated positively to nutrient levels and Microcystis biomass during bloom developing in 2013 from June to August.Temporally,the cellular content of total microcystin was lowest when the bloom peaked in intensity.Our results suggest that lake eutrophication not only results in cyanobacterial blooms,but may also increase the proportion of toxic Microcystis species and their cell-bound MCs contents(i.e.microcystin cell quotas) under mild eutrophication.The present investigation provided molecular evidence for the selection of MC-producing and non-MC-producing genotypes.The current study provides new evidence advocating the monitoring of partitions of large lakes when studying cyanobacteria and toxin-contaminated freshwaters,which will be beneficial for both water agencies and water researchers.展开更多
基金National Natural Science Foundation of China(grant nos.31072239,31270213)Knowledge Innovation Program of the Chinese Academy of Sciences(grant no.KSCX2-EW-Z-3)StateKey Laboratory of Freshwater Ecology&Biotechnology Program(grant no.2011FBZ12)
文摘The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,China.Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC.Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis.Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length.Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80infectious units per cell,respectively.Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis revealed four major structural proteins.These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
基金Supported by the Aquaculture and Fisheries Collaborative Research Support Program of USAID (No. 1366)the Shanghai Rising-Star Program (No. 08QA1405900)+1 种基金the Innovation Program of Shanghai Municipal Education Commission (No. 09YZ277)the Shanghai Leading Academic Discipline Project (No. Y1101)
文摘Cyanobacterial blooms in eutrophic freshwater systems are a worldwide problem, creating adverse effects for many aquatic organisms by producing toxic mierocystins and deteriorating water quality. In this study, microcystins （MCs） in Microcystis aeruginosa, and Daphnia magna exposed to M. aeruginosa, were analyzed by HPLC-MS, and the effects of M. aeruginosa on D. magna were investigated. When D. magna was exposed to M. aeruginosa for more than 2 h, Microcystin-LR （MC-LR） was detected. When exposed to 1.5× 10^6, 3× 10^6, 0.75× 10^7, and 1.5× 10^7 cell/mL of M. aeruginosa for 96 h, average survival of D. magna for treatments were 23.33%, 33.33%, 13.33%, 16.67%, respectively, which were significantly lower than the average 100% survival in the control group （P 〈 0.05）. The adverse effects ofM. aeruginosa on body length, time for the first brood, brood numbers, gross fecundity, lifespan, and population growth olD. magna were density-dependent. These results suggest that the occurrence of M. aeruginosa blooms could strongly inhibit the population growth of D. magna through depression of survival, individual growth and gross fecundity. In the most serious situations, M. aeruginosa blooms could undermine the food web by eliminating filter-feeding zooplankton, which would destroy the ecological balance of aquaculture water bodies.
基金Supported by the Major Science and Technology Program for Water Pollution Control and Treatment(Nos.2012ZX07103-004-02,2015ZX07204002)the Henan Institute of Engineering Innovation Team Building Program(No.CXTD2014005)+2 种基金the Henan Province Science Projects for Colleges and Universities(No.15A610011)the City Science and Technology Project(No.20140659)the Fund Project for Doctor(No.D2014009)
文摘Although biomass variations in Microcystis and microcystin have been widely reported,few studies have addressed whether different trophic states of natural lake water affect the spatial-temporal variations in abundances of microcystin-producing Microcystis in a given bloom.In this study,we used a harmful algal bloom in Chaohu Lake,eastern China,as an example to investigate the mutual relationship between different nutrient states and environmental factors,and the impact on Microcystis.Overall,cyanobacteria and Microcystis were more abundant in the middle and western parts of the lake under high nutrients levels,while in the eastern part,nutrient concentrations were low enough to limit biomass,and their fluctuations affected the contents of toxic Microcystis.Moreover,microcystin concentration was correlated positively to nutrient levels and Microcystis biomass during bloom developing in 2013 from June to August.Temporally,the cellular content of total microcystin was lowest when the bloom peaked in intensity.Our results suggest that lake eutrophication not only results in cyanobacterial blooms,but may also increase the proportion of toxic Microcystis species and their cell-bound MCs contents(i.e.microcystin cell quotas) under mild eutrophication.The present investigation provided molecular evidence for the selection of MC-producing and non-MC-producing genotypes.The current study provides new evidence advocating the monitoring of partitions of large lakes when studying cyanobacteria and toxin-contaminated freshwaters,which will be beneficial for both water agencies and water researchers.
