A method for dispersive liquid-liquid microextraction of glibenclamide on model mixtures with urine has been developed. The extraction conditions have been optimized and the influence of extractants and dispersing age...A method for dispersive liquid-liquid microextraction of glibenclamide on model mixtures with urine has been developed. The extraction conditions have been optimized and the influence of extractants and dispersing agent for allocation of toxicant from biosubstrate has been experimentally established. The method of TLC （thin layer chromatography） screening in order to remove endogenous and exogenous substances has been developed. The method of IR-spectroscopy for confirmatory analysis has been used. The combination of the two methods of analysis allows identifying glibenclamide quickly and reliably isolated from bioliquid and reducing the risk of false-positive results.展开更多
The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TL...The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TLC),a simple and classic approach for qualitatively characterizing and examining quality markers of natural products,has been widely used in the characterization and quality control of traditional Chinese medicines.Zaoren Anshen(ZRAS)capsule,prepared from three medicinal herbs of fried Ziziphi Spinosae Semen,Salvia Miltiorrhiza Radix et Rhizoma,and vinegar-processed Schisandrae Chinensis Fructus,is a famous PCM in China for the treatment of insomnia,amnesia,and dizziness in clinical practice.However,no effective method is available so far for simultaneous identification and examination of all the three medicinal herbs of ZRAS capsule.In the present study,we developed a TLC method via twice-development and visualization by UV light or chromogenic agent,which could be used for simultaneous qualitative identification of all the three medicinal herbs of ZRAS capsule in one plate.Moreover,the sample preparation method was optimized.The developed TLC method was rapid,simple,low-cost,and effective,and thus it could be used for quality control of ZRAS capsule.展开更多
文摘A method for dispersive liquid-liquid microextraction of glibenclamide on model mixtures with urine has been developed. The extraction conditions have been optimized and the influence of extractants and dispersing agent for allocation of toxicant from biosubstrate has been experimentally established. The method of TLC （thin layer chromatography） screening in order to remove endogenous and exogenous substances has been developed. The method of IR-spectroscopy for confirmatory analysis has been used. The combination of the two methods of analysis allows identifying glibenclamide quickly and reliably isolated from bioliquid and reducing the risk of false-positive results.
基金National Key Research and Development Program of China(Grant No.2018YFC1707300)。
文摘The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TLC),a simple and classic approach for qualitatively characterizing and examining quality markers of natural products,has been widely used in the characterization and quality control of traditional Chinese medicines.Zaoren Anshen(ZRAS)capsule,prepared from three medicinal herbs of fried Ziziphi Spinosae Semen,Salvia Miltiorrhiza Radix et Rhizoma,and vinegar-processed Schisandrae Chinensis Fructus,is a famous PCM in China for the treatment of insomnia,amnesia,and dizziness in clinical practice.However,no effective method is available so far for simultaneous identification and examination of all the three medicinal herbs of ZRAS capsule.In the present study,we developed a TLC method via twice-development and visualization by UV light or chromogenic agent,which could be used for simultaneous qualitative identification of all the three medicinal herbs of ZRAS capsule in one plate.Moreover,the sample preparation method was optimized.The developed TLC method was rapid,simple,low-cost,and effective,and thus it could be used for quality control of ZRAS capsule.
