Study on Cryopreservation of Porphyra yezoensis Conchocelis

Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol(EG),propylene glycol(PEG),sorbitol and sucrose were developed. The effect of prefreezing at - 40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL-1 sorbitol exhib-ited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20 min at - 40℃ before stored in liquid nitrogen,and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.

Thermal contrast between the Tibetan Plateau and tropical Indian Ocean and its relationship to the South Asian summer monsoon

The land-sea thermal contrast is an important driver for monsoon interannual variability and the monsoon onset.The thermal contrast between the Tibetan Plateau and the tropical Indian Ocean at the mid-upper troposphere is proposed as a thermal contrast index(TCI)for South Asian monsoon.The authors investigate the TCI associated with South Asian summer monsoon(SASM)intensity and SASM onset.It is observed that the TCI considering the Tibetan Plateau and tropical Indian Ocean demonstrates a stronger and closer correlation with SASM intensity(0.87)than either the Tibetan Plateau(0.42)or tropical Indian Ocean(-0.60)singly.It is implied that the TCI could preferably represent the impact of land-sea thermal condition on SASM activity.Further analysis reveals that the evolution of TCI is related to the SASM onset.The TCI is almost always larger in early onset years than it is in late onset years during the period before SASM onset.In addition,the change of the pentad-by-pentad increment of TCI leads the SASM variation.The correlation coefficient between the TCI increment and SASM index reaches a maximum when the TCI increment leads by 15 pentads.The results of this study show that the TCI plays an important role in SASM activities and is a potential indicator for SASM onset forecasting.

Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus

The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.

Spatio-temporal variability of periphytic protozoa related to environment in the Niyang River,Tibet,China

The Niyang River, a main tributary of the Yarlung Zangbo River, is an important and typical plateau fiver ecosystem in Tibet, China. At present, few studies have focused on its aquatic living resources and fiver ecology. In this study, the composition, abundance, and diversity of periphytic protozoa were investigated across four seasons from 2008 to 2009 to better understand their spatio-temporal patterns and relationship to the environment. Our investigation shows that periphytic protozoa in the Niyang River contained 15 genera, belonged to Tubulinea, Alveolata, Discosea and Rhizaria, Alveolata possessed most genera, up to nine, with highest share in abundance, exceeding 50%, Difflugia and Glaucoma were dominant genera. Moreover, four diversity indices of periphytic protozoa, including species richness, total abundance, Shannon-Wiener diversity index and Pielou＇s evenness index, displayed a significant descending trend as the seasons continued, in the order of winter, spring, summer and autumn; with a significant difference existing between winter and summer （or autumn） for Shannon-Wiener diversity index and species richness （P〈0.05）. Four of these diversity indices also presented a V-shaped pattern between the upper middle course of the Niyang River and the confluence of the Niyang River and Yarlung Zangbo River, with the lowest value occurred in the middle course of the Niyang River. However, no significant variation was found through the Niyang River （P〉0.05）. In addition, canonical correlation analysis （CCA） shows that the densities of Difflugia, Glaucomais, Enchelydium, Cyphoderia, and Enchelys correlate with water temperature, alkalinity, hardness, pH, and dissolved oxygen, respectively. Lastly, the relationship between periphytic protozoa diversity and the environmental factors of the Niyang River can be predicted using classification and regression trees （CART） annalysis, which suggests that the total abundance and Shannon-Wiener diversity index would be higher when the elevation is above 3 308 m. On the other hand, the Shannon-Wiener diversity index and Pielou＇s evenness index would be lower when pH and ammoniacal nitrogen have lower or higher values. Finally yet importantly, close attention should be paid to periphytic protozoa and its environment to ensure sustainable development of the Niyang River ecosystem.

Petrogenesis of high-Mg# Cenozoic volcanic rocks of southern Qiangtang area, Tibetan Plateau： geochemical and Sr-Nd isotopic evidence

The Nadingcuo volcanic rock suite is the most volmninous Cenozoic volcanic suite in the southern Qiangtang area of the northern Tibetan Plateau. These high-K calc-alkaline volcanic rocks were formed between 36 and 34 Ma, characterized by high Mg# values, high concentrations of TiO2 and P205, 87Sr/S6Sr ratios of 0. 704682--0. 706 112, and aNd（t） values of - 1.2 to 1.6. There is a lack of reasonable explanations for sour- cing and origin of magmas that formed the rocks with high Mg# values and TiO2 and P2O5 enrichments, which makes the previous research results still controversial. This study reviews the geochemical characteristics of Nadingcuo volcanic rocks and the data we have newly found in our fieldwork. We give some new interpretation to the magmatic evolution of the basaltic magmas in the discussed area dominated by fractional crystallization. The geochemistry of trachyandesite and trachyte units in the studied area is indicative of formation from mantle- derived magmas that mixed with crustal materials. The high values of Mg# and TiO2 and P205 enrichment in these units are evident to show the mixing between mantle-derived magmas with -30-40 wt.% rhyolitic melt or assimilation of a similar amount of felsic rocks. The geochemistry of basaltic rocks in the area also suggests that the Nadingcuo basalts may have been derived from an ocean island basalt （OIB） -type source that contained and was mixed with ancient mantle wedge derived material, indicating that a 36-34 Ma asthenospheric upwelling e- vent in the Qiangtang area may relate to the northward subduction of Indian lithospheric mantle and the south- ward subduction of Asian lithospherie mantle. This upwelling of asthenospherie material was centered in the southern Qiangtang area between 36 and 34 Ma, while the northward movement of the Indian Craton caused this upwelling mantle flow to continuously migrate northward, resulting in the current centering of this upwelling in the Hoh Xil-Kunlun region.

EFFECTS OF THE ATMOSPHERIC COLD SOURCE OVER THE TIBETAN PLATEAU ON THE QUASI 4-YEAR OSCILLATION OF OCEAN-ATMOSPHERIC-LAND INTERACTION

Using correlation analyses, composite analyses, and singular value decomposition, the relationship between the atmospheric cold source over the eastern Tibetan Plateau and atmospheric/ocean circulation is discussed. In winter, the anomaly of the strong (weak) atmospheric cold source over the eastern plateau causes low-level anomalous north (south) winds to appear in eastern China and low-level anomaly zonal west (east) winds to prevail in the equatorial Pacific from spring to autumn. This contributes to the anomalous warm (cold) sea surface temperature the following autumn and winter. In addition, the anomalous variation of sea surface temperature over the equatorial middle and eastern Pacific in winter can influence the snow depth and intensity of the cold source over the plateau in the following winter due to variation of the summer west Pacific subtropical high.

An approach to the age of Huanghe River in shelf regions of Yellow Sea and Bohai Sea

There are two different opinions on the formation history of Huanghe (Yellow) River. One postulates that Huanghe River might have come into existence before Tertiary. The other supposes that it joined up into a long river only in the last stage of Late Pleistocene. The appearance of Huanghe River is believed to have close relation to the uplifting of Tibetan Plateau. It is not likely that it could have come into being before its high elevation riverhead was formed. Today Huanghe River occurred probably during the recession of the sea in glacial periods. In the last glacial age, the climate was very harsh in the area north of the modern estuary of Changjiang (Yangtse) River; some areas were permafrost and the others barren deserts. At that time, eolation was the major exogenic force on exposed shelf. Beginning from 12 Ka BP, the global climate warmed up, resulting in gradual disappearance of continental mountain glaciers retreated, and sea level rose. Consequently, Huanghe River was replenished with water to become modern river system. With continued rising of sea level, Huanghe River delta moved continuously eastward.

Analysis of Longitudinal Profiles along the Eastern Margin of the Qinghai-Tibetan Plateau

Resulting from the collision of the Eurasian and Indian plates, the Qinghai-Tibetan Plateau is commonly known as the ＇roof of the world＇. Collectively the Yarlung Tsangpo, Nu, Laneang, Yangtze, Yalong, and Yellow River basins drain the eastern margin of the plateau. In this paper, we utilize Shuttle Radar Topography Mission elevation data to examine morphometric and relief attributes of these basins to reveal insights rates of incision. A robust into tectonic activity and technique using Maflab is proposed to alleviate errors associated with SRTM data in the derivation of river longitudinal profiles. Convex longitudinal profiles are interpreted to be a product of uplift rates that exceed rates of channel incision along the entire margin of the Qinghai- Tibetan Plateau. Highest relief towards the south reflects extensive fluvial incision. High relief is also prominent along major active faults. Erosion patterns are related to distance from knickpoints. Highest rates of erosion and incision are evident towards the south, with decreasing values towards the north, suggesting a link between tectonic activity and erosion.

ON THE RELATIONSHIPS BETWEEN THE SUMMER RAINFALL IN CHINA AND THE ATMOSPHERIC HEAT SOURCES OVER THE EASTERN TIBETAN PLATEAU AND THE WESTERN PACIFIC WARM POOL

The relationships between the summer rainfall in China and the atmospheric heat sources over the eastern Tibetan Plateau and the western Pacific warm pool were analyzed comparatively, using the NCEP/NCAR reanalysis daily data. The strong (weak) heat source in summer over the eastern Tibetan Plateau will lead to abundant (scarce) summer rainfall in the Yangtze River basin, and scarce/abundant summer rainfall in the eastern part of Southern China. While the strong (weak) heat source in summer over the western Pacific warm pool will lead to another pattern of abundant (scarce) summer rainfall in the middle-lower reaches of the Yangtze River and scarce (abundant) summer rainfall in Southern China and in the region of northern Jiangsu to southern Shandong. Comparatively, the heat source over the eastern Tibetan Plateau affects a larger area of summer rainfall than the heat source over the western Pacific. In both cases of the heat source anomalies over the eastern Tibetan Plateau and over the western Pacific, there exist EAP-like teleconnection patterns in East Asia. The summer rainfall in China is influenced directly by the abnormal vertical motion, which is related closely to the abnormal heat sources in the atmosphere. The ridge line of the western Pacific High locates far south (north) in summer in the case of strong (weak) heat sources over the two areas mentioned above.

Effect of lake surface temperature on the summer precipitation over the Tibetan Plateau

There are numerous lakes on the Tibetan Plateau(TP),but the role of lake temperature in precipitation over the TP remains unclear.Here the Weather Research and Forecasting(WRF) model was used to detect the impact of lakes on summer rainfall.Three test cases were used to evaluate the effect of lakes surface temperature(LSTs) on precipitation variability.The three cases used different methods to determine initial LSTs,including using sea surface temperature data(SST),the WRF inland water module(avg_tsfc),and a lake model.Results show that when precipitation was stimulated over the TP,LSTs cannot be initialized using SST,which led to large discrepancies of precipitation.Compared with the simulations,the simulated precipitation were improved obviously with LSTs using avg_tsfc,indicating that LSTs have an considerable influence on determining precipitation over the TP.Due to a lack of observational data,the lake scheme does not improve on rainfall simulation,but does effectively simulate precipitation pattern over lakes,such as rainfall over the lakes was dominated by convection during the nighttime.Though the simulated precipitation using SST to initialize LSTs caused largediscrepancies,it suggested that precipitation increase especially convective precipitation with increase in LSTs,which confirmed that the moisture from lakes cannot be neglected over the TP.Generally,it was necessary to monitor the LSTs for accurate weather and climate prediction over the TP.

Role of ENSO in the interannual relationship between Tibetan Plateau winter snow cover and Northwest Pacific tropical cyclone genesis frequency

Previous studies have revealed a significantly negative correlation between prior winter snow cover over the Tibetan Plateau （TPSC） and tropical cyclone genesis frequency （TCF） over the western North Pacific （WNP） in the following typhoon season. This study revisited this relationship based on long-term observational data. The results showed that the interannual correlation between TCF over the WNP and TPSC experienced a shift in the early 1990s. This correlation is significant during only 1993-2012 and is considerably weak during 1976-1992. The possible reasons causing the shift were examined further, and the results demonstrated that the central Pacific （CP） E1 Nifio-Southern Oscillation （ENSO） has played a vital role in intensifying the interannual relationship between TCF over the WNP and TPSC since the early 1990s. During 1993-2012, TPSC was negatively related to CP ENSO. When TPSC was higher than （lower than） normal, CP ENSO was often in its cold （warm） phase. Such a combination remarkably enhances the relationship of TPSC with the zonal land-sea thermal difference and thus with the summer monsoon over the WNE Additionally, it enhances the modulation of TPSC on the dynamical environments controlling TCF. As a result, the linkage between TPSC and TCF was significantly strengthened in this period. In sharp contrast, due to the weak relationship between TPSC and ENSO followed by the weak modulation of TPSC on the summer monsoon over the WNP and the dynamical environment during 1976-1992, the linkage between TPSC and TCF was weak during this time period. The results from additional dynamical diagnostic analyses further showed that during 1993-2012 CP ENSO modulated the barotropic energy conversion of zonal winds over the WNP, contributing to the intensified relationship between TPSC and TCF. These results will improve seasonal forecasting of tropical cyclone activity over the WNP.