Down hairs were sampled from twenty two winter skins of the Tibetan antelope (Pantholops hodgsonii). For each individual,5 down hairs were collected from each sampling site including vertex,scruff,mid-back,buttock,mid...Down hairs were sampled from twenty two winter skins of the Tibetan antelope (Pantholops hodgsonii). For each individual,5 down hairs were collected from each sampling site including vertex,scruff,mid-back,buttock,mid-belly,tail,forelimb and hindlimb and subjected to morphological examination. Morphological indices of the hairs were measured,including hair curled length,hair uncurled length,hair diameter,scale height,scale width,longitudinal scale density,and then crimp ratio and scale aspect ratio were calculated. The results indicated that scales throughout the hair shaft were coronal type,like overlay of cups. The hair diameter in all sampling sites was between 11.69±1.25 μm and 12.41±0.79 μm. Both the curled length and uncurled length of the hairs on vertex,scruff,mid-back,buttock and midbelly were significantly longer than on tail and limbs (P<0.05). Crimp ratio varied between 11.62±2.78% and 19.79±3.22%,and crimp ratio on vertex was significantly higher than on other sites (P<0.05). Scale height varied between 20.55±4.79 μm and 25.58±5.86 μm,and scale height on limbs was significantly lower than on other sites (P<0.05); Scale widths ranged from 10.09±1.49 μm to 11.33±1.98 μm amongst all sampling sites. Scale widths on vertex and hind limb were significantly lower than on other sites (P<0.05); Scale aspect ratio varied between 2.15±0.60 and 2.56±0.81,where the ratio on mid-back were significantly higher than on other sites (P<0.05); The longitudinal scale density ranged from 38±3 scales/mm to 46±9 scales/mm,where the tail was significantly higher than other sites except hind limb (P<0.05). Morphological characteristics of down hairs of Tibetan antelope and their differentiation in different body sites are to meet the different functional requirements.展开更多
文摘藏羚Pantholops hodgsonii是藏羚属Pantholops现存的唯一物种,由于Y染色体基因较保守,基于近缘物种的Y染色体多态性遗传位点筛选受到了很大的限制。本研究对15份藏羚新鲜组织样品进行基因组重测序,生物信息分析筛选出Y染色体雄性特异区(MSY)对应的scaffolds,对部分SNP及SSR位点进行多态性验证。共比对出44个scaffolds,以其中序列最长、候选变异位点最多且最完整的KE113803.1为参考,对其中190个SNP变异位点设计引物进行验证,共获得45条MSY DNA序列,其中15对引物扩增到的11898 bp DNA序列中检测到27个SNP位点;同时对KE113803.1中除单核苷酸重复以外的134个SSR位点设计引物并验证多态性,筛选出56个Y-SSR位点,其中5个具有多态性。本研究结果为后续分析藏羚Y染色体遗传多样性及父系遗传奠定了良好基础。
文摘Down hairs were sampled from twenty two winter skins of the Tibetan antelope (Pantholops hodgsonii). For each individual,5 down hairs were collected from each sampling site including vertex,scruff,mid-back,buttock,mid-belly,tail,forelimb and hindlimb and subjected to morphological examination. Morphological indices of the hairs were measured,including hair curled length,hair uncurled length,hair diameter,scale height,scale width,longitudinal scale density,and then crimp ratio and scale aspect ratio were calculated. The results indicated that scales throughout the hair shaft were coronal type,like overlay of cups. The hair diameter in all sampling sites was between 11.69±1.25 μm and 12.41±0.79 μm. Both the curled length and uncurled length of the hairs on vertex,scruff,mid-back,buttock and midbelly were significantly longer than on tail and limbs (P<0.05). Crimp ratio varied between 11.62±2.78% and 19.79±3.22%,and crimp ratio on vertex was significantly higher than on other sites (P<0.05). Scale height varied between 20.55±4.79 μm and 25.58±5.86 μm,and scale height on limbs was significantly lower than on other sites (P<0.05); Scale widths ranged from 10.09±1.49 μm to 11.33±1.98 μm amongst all sampling sites. Scale widths on vertex and hind limb were significantly lower than on other sites (P<0.05); Scale aspect ratio varied between 2.15±0.60 and 2.56±0.81,where the ratio on mid-back were significantly higher than on other sites (P<0.05); The longitudinal scale density ranged from 38±3 scales/mm to 46±9 scales/mm,where the tail was significantly higher than other sites except hind limb (P<0.05). Morphological characteristics of down hairs of Tibetan antelope and their differentiation in different body sites are to meet the different functional requirements.
