Adult male rats were treated orally with monomer T_4(Tripchlorolide) isolated from Tripterygium wilfordii, at the dose of 50μg/kg/day,6 days/week for6 weeks.Ultrathin section and freeze etching replica of seminifero...Adult male rats were treated orally with monomer T_4(Tripchlorolide) isolated from Tripterygium wilfordii, at the dose of 50μg/kg/day,6 days/week for6 weeks.Ultrathin section and freeze etching replica of seminiferous tubules and epididymal spermatozoa were examined with elec-tron microscope. The results showed that the spemiogenesis was inhibited T_4 in seminiferous tu-bules. However,the damage and disruption of the spermatozoa were more serious in the epididymis.Damage of the structure and function ot microtubule and microfilament may be the chief reason for sperm damage. Sperm membrane was also very sensitive to the treatiment of monomer T_4.展开更多
In the present study,we aimed to investigate the chemical constituents from Embelia laeta.The constituents were separated by various column chromatography,including silica gel,ODS gel,MCI gel,Sephadex LH-20,and semipr...In the present study,we aimed to investigate the chemical constituents from Embelia laeta.The constituents were separated by various column chromatography,including silica gel,ODS gel,MCI gel,Sephadex LH-20,and semipreparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from E.laeta and identified as(6R,9R)-1-Oxo-α-ionol-9-O-β-D-goucopyranoside(1),(+)-Pinoresinol(2),Leptolepisol D(3),Kaempferol(4),Kaempferol-3-α-L-Ramnopyranoside(5),Kaempferol-3-β-D-(6-O-trans-p-coumaroyl) glucopyranoside(6),Bis(2-ethylhexyl) phthalate(7),2-Benzenedicarboxylic diisobutyl ester(8),Caffeic acid methyl ester(9),Paldehyde(10),Coniferaldehyde(11),Caffeic acid(12)and 5-Hydroxymethyl furfural(13),respectively.All the compounds were isolated from E.laeta for the first time.展开更多
文摘Adult male rats were treated orally with monomer T_4(Tripchlorolide) isolated from Tripterygium wilfordii, at the dose of 50μg/kg/day,6 days/week for6 weeks.Ultrathin section and freeze etching replica of seminiferous tubules and epididymal spermatozoa were examined with elec-tron microscope. The results showed that the spemiogenesis was inhibited T_4 in seminiferous tu-bules. However,the damage and disruption of the spermatozoa were more serious in the epididymis.Damage of the structure and function ot microtubule and microfilament may be the chief reason for sperm damage. Sperm membrane was also very sensitive to the treatiment of monomer T_4.
文摘In the present study,we aimed to investigate the chemical constituents from Embelia laeta.The constituents were separated by various column chromatography,including silica gel,ODS gel,MCI gel,Sephadex LH-20,and semipreparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from E.laeta and identified as(6R,9R)-1-Oxo-α-ionol-9-O-β-D-goucopyranoside(1),(+)-Pinoresinol(2),Leptolepisol D(3),Kaempferol(4),Kaempferol-3-α-L-Ramnopyranoside(5),Kaempferol-3-β-D-(6-O-trans-p-coumaroyl) glucopyranoside(6),Bis(2-ethylhexyl) phthalate(7),2-Benzenedicarboxylic diisobutyl ester(8),Caffeic acid methyl ester(9),Paldehyde(10),Coniferaldehyde(11),Caffeic acid(12)and 5-Hydroxymethyl furfural(13),respectively.All the compounds were isolated from E.laeta for the first time.
