由灰葡萄孢Botrytis cinerea引起的梨灰霉病是梨果储藏中危害严重的病害,不仅影响果实品质,还可造成严重经济损失（Romanazzi et al.,2016）。生物防治作为环保、安全的方法日益受到重视。链霉菌因其种类繁多、代谢产物丰富、作用方式...由灰葡萄孢Botrytis cinerea引起的梨灰霉病是梨果储藏中危害严重的病害,不仅影响果实品质,还可造成严重经济损失（Romanazzi et al.,2016）。生物防治作为环保、安全的方法日益受到重视。链霉菌因其种类繁多、代谢产物丰富、作用方式多样等优点,已成为重要的生物防治微生物资源,但其在梨灰霉病生物防治中的研究应用较少（孙平平等,2016）。展开更多
Total protein extraction of Streptomyces luteogriseus in different fermentation times was analyzed in order to discover the enzymes associated with Maituolaimysin biosynthesis, make the biosynthesis pathway clear, and...Total protein extraction of Streptomyces luteogriseus in different fermentation times was analyzed in order to discover the enzymes associated with Maituolaimysin biosynthesis, make the biosynthesis pathway clear, and enhance its production. Two-dimensional gel electrophoresis analysis showed that 8 proteins were up-expressed, 5 were down-expressed, 6 were new-expressed and 6 disappeared in the protein extraction of 48 h compared with that of 36 h. Fifteen of the different expression proteins were analyzed by Matrix-Assisted Laser Desorption Ionization(MALDI)Time-of-Flight(TOF) mass spectrum and 4 of them were identified. Among the 4 identified proteins, one was involved in protein biosynthesis; one was associated with Streptomyces differentiation; one was a key enzyme in TCA circle and another one was a hypothetical protein whose function was unknown.Investigation of the function of these identified proteins suggested that they all had direct or indirect relations to Maituolaimysin biosynthesis, and especially, the protein Clp2 could accelerate the differentiation of Streptomyces, which would directly result in Maituolaimysin biosynthesis.展开更多
文摘由灰葡萄孢Botrytis cinerea引起的梨灰霉病是梨果储藏中危害严重的病害,不仅影响果实品质,还可造成严重经济损失（Romanazzi et al.,2016）。生物防治作为环保、安全的方法日益受到重视。链霉菌因其种类繁多、代谢产物丰富、作用方式多样等优点,已成为重要的生物防治微生物资源,但其在梨灰霉病生物防治中的研究应用较少（孙平平等,2016）。
文摘Total protein extraction of Streptomyces luteogriseus in different fermentation times was analyzed in order to discover the enzymes associated with Maituolaimysin biosynthesis, make the biosynthesis pathway clear, and enhance its production. Two-dimensional gel electrophoresis analysis showed that 8 proteins were up-expressed, 5 were down-expressed, 6 were new-expressed and 6 disappeared in the protein extraction of 48 h compared with that of 36 h. Fifteen of the different expression proteins were analyzed by Matrix-Assisted Laser Desorption Ionization(MALDI)Time-of-Flight(TOF) mass spectrum and 4 of them were identified. Among the 4 identified proteins, one was involved in protein biosynthesis; one was associated with Streptomyces differentiation; one was a key enzyme in TCA circle and another one was a hypothetical protein whose function was unknown.Investigation of the function of these identified proteins suggested that they all had direct or indirect relations to Maituolaimysin biosynthesis, and especially, the protein Clp2 could accelerate the differentiation of Streptomyces, which would directly result in Maituolaimysin biosynthesis.
