[Objective] The research aimed to improve the method of isolation of algae.[Method] The samples were cultured in liquid medium after pre-culture in natural medium.[Result]12 species of algae were isolated from the sam...[Objective] The research aimed to improve the method of isolation of algae.[Method] The samples were cultured in liquid medium after pre-culture in natural medium.[Result]12 species of algae were isolated from the sample.[Conclusion] The method is useful and easy to be handled.展开更多
This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabric...This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles' or algae's volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment.展开更多
文摘[Objective] The research aimed to improve the method of isolation of algae.[Method] The samples were cultured in liquid medium after pre-culture in natural medium.[Result]12 species of algae were isolated from the sample.[Conclusion] The method is useful and easy to be handled.
基金support from the Fundamental Research Funds for the Central Universities (2011QN105, 2011ZD014)the Dalian Science and Technology Foundation (2011J21DW005) to SONG YongXin+2 种基金National Science & Technology Pillar Program of China in 2010 (2010BAC68B02)Liaoning Science & Technology Program (2007405010) to SUN YeQingthe Natural Sciences and Engineering Research Council of Canada through a research grant to LI DongQing
文摘This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles' or algae's volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment.
