A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Library Construction Kit.The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplifie...A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Library Construction Kit.The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplified library was 3.0×109 pfu mL-1.The percentages of the recombinant clones of primary and amplified libraries were over 98%.The insert sizes were longer than 400 bp with an average of 1000 bp.A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin I gene.This library provided a useful resource for the functional genomic research of F.chinensis.展开更多
基金supported by grants from the National Key Technology Research and Development Program (2006-BADO1A13)the earmarked fund for Modern Agro-industry Technology Research System
文摘A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Library Construction Kit.The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplified library was 3.0×109 pfu mL-1.The percentages of the recombinant clones of primary and amplified libraries were over 98%.The insert sizes were longer than 400 bp with an average of 1000 bp.A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin I gene.This library provided a useful resource for the functional genomic research of F.chinensis.
