蓖麻蚕核型多角体病毒包涵体蛋白的提纯及SDS-PAGE分析

对蓖麻蚕核型多角体病毒 (PhilosamiacynthiariciniNuclearPolyhedrosisViruses)的多角体蛋白 polyhedrin的碱解性质以及分子量等进行了分析。结果发现 :多角体加热灭活和碱解后 ,经等电点沉淀的 polyhedrin通过凝胶电泳仍存在较多的小分子量蛋白带 ,表明通常的 70℃加热不能完全灭活碱性蛋白酶 ;在电泳图谱的主带polyhedrin一侧尚有一伴随的的卫星带 ,可能提示包涵体的外膜蛋白。Sephadex -G2 0 0柱层析纯化的 polyhedrin经SDS -PAGE分析 ,多角体蛋白有 4条带 ,其中主带分子量约为 30KD ,另有一带处于 6 1KD位置 ,推测为 polyhedrin的二聚体 ,说明polyhedrin多聚体在杆状病毒包涵体中的普遍性。

Prevalence of Muscardine Disease in Different Silkworm Hybrid, Bombyx Mori L. under Agro-climatic Conditions in Aurangabad （M.S.）, India

A study was carried out in Dr. Babasaheb Ambedkar Marathwada University Campus Aurangabad, Maharashtra State, India for analyzing the prevalence of Muscardine disease during rainy, winter and summer season in bivoltine x bivoltine hybrids viz. CSR2 × CSR4, CSR4 × CSR2 and multi × bivoltine hybrid PM × CSR2. Observation on Muscardine disease was recorded till the onset of spinning. Analysis of results shows that disease prevalence was more in bivoltine x bivoltine hybrids compare to multi × bivoltine hybrids. PM × CSR2 was found to be more resistant towards Muscardine disease compared to other hybrids under agro-climatic conditions of Aurangabad.