Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic mater...Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.展开更多
An innovative one-step semi-solid processing technique of A356 Al alloy,the serpentine channel pouring rheo-diecasting process （SCRC）,was explored.The mechanical properties and microstructures of the tensile samples...An innovative one-step semi-solid processing technique of A356 Al alloy,the serpentine channel pouring rheo-diecasting process （SCRC）,was explored.The mechanical properties and microstructures of the tensile samples made by the SCRC technique were tested in the as-cast and T6 heat treatment conditions.The experimental results show that the as-cast ultimate tensile strength can reach about 250MPa and the elongation is 8.6%？13.2%.The ultimate tensile strength can increase approximately 30% higher than that of the as-cast one but there is some slight sacrifice of the plasticity after T6 heat treatment.Under these experimental conditions,the semi-solid A356 Al alloy slurry with primary α1（Al） grains,which have the shape factor of 0.78？0.89 and the grain diameter of 35？45μm,can be prepared by the serpentine channel pouring process.The primary α2（Al） grains are very fine during the secondary solidification stage.Compared with the conventional HPDC process,the SCRC process can improve the microstructures and mechanical properties of the tensile test samples.The advantages of the SCRC process include easily incorporating with an existing HPDC machine,cancelling the preservation and transportation process of the semi-solid alloy slurry,and a higher cost performance.展开更多
Aim To investigate the anticancer activity of two new cytotoxins from thevenom of Agkistrodon acutus. Methods The venom was isolated by FPLC column chromatography consistingof DEAE Sepharose FF and Source 30S. The cyt...Aim To investigate the anticancer activity of two new cytotoxins from thevenom of Agkistrodon acutus. Methods The venom was isolated by FPLC column chromatography consistingof DEAE Sepharose FF and Source 30S. The cytotoxic activity on tumor cells was detected by MITmethod. Purity and molecular weight were determined by SDS-PAGE (silver staining). Their stabilitiesto temperature and pH were also detected. Results Two pure cytotoxins named ACTX-6 and ACTX-8 wereobtained. Their molecular weights are 98 kDa and 27 kDa, respectively. ACTX-6 consists of twosubunits bonded together by disulfide bonds. Conclusion ACTX-6 and ATCX-8 have highest inhibitoryactivity on lung cancer cell A549. ACTX-6 is stable to heat while ACTX-8 not. ACTX-6 is stablebetween pH 7-9 and ACTX-8 between pH 6 - 9.展开更多
The relationship between viscosity/yield stress and flotation rate in different chalcopyrite and lizardite concentrations was investigated by zeta potential measurements,rheological tests,flotation experiments,and Der...The relationship between viscosity/yield stress and flotation rate in different chalcopyrite and lizardite concentrations was investigated by zeta potential measurements,rheological tests,flotation experiments,and DerjguinLandau-Verwey-Overbeek theoretical calculation.Results indicated that the concentration of minerals would affect the viscosity and yield stress of the slurry.That is,the viscosity/yield stress in the slurry rises accordingly when the concentration of minerals increases.The increase in viscosity/yield stress in slurry is also unfavorable to the enrichment of chalcopyrite but is conducive to the entrainment of lizardite in either single or binary system.Specifically,the flotation rate of chalcopyrite decreases while that of lizardite increases with the rise in viscosity/yield stress.展开更多
Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respect...Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respectively. The change of antigenic activities and coagulating activities of Defibrase in the same buffer solutions (pH 6, 7 and 8, with the exception of pH 3.6) showed similar tendency to decline with the time. Concentrated Defi-brase was relatively stable at neutral pH 6~7, more than 95% of its initial activities (100BUmL-1) was kept after a 10-day storage at 40 oC, whereas in pH 3.6 and pH 9 buffer solutions, diluted Defibrase was very labile. Addition of Triton X-100 or bovine serum albumin could effectively prevent loss of Defibrase by minimizing adsorption of De-fibrase to plastic surface (P<0.005). Concentration of Defibrase could also affect its stability in aqueous solutions.展开更多
Cyanea nozakii Kishinouye （C. nozakii）, a giant cnidarian of the class Scyphomedusae, order Semaeostomeae and family Cyaneidae, is widely distributed in the East China Sea, the Yellow Sea and the Bohai Sea, and is a...Cyanea nozakii Kishinouye （C. nozakii）, a giant cnidarian of the class Scyphomedusae, order Semaeostomeae and family Cyaneidae, is widely distributed in the East China Sea, the Yellow Sea and the Bohai Sea, and is abundant from late summer to early autumn. Venom produced by C. nozakii during mass agglomerations can contaminate seawater resulting in death of the halobios and seriously damage commercial fisheries. Swimmers and fishermen commonly suffer painful stings from this j ellyfish, resulting in local edema, tingling, breathing difficulties, depressed blood pressure and even death. Such effects arise from the complex mixture of biologically active molecules that make up jellyfish venom. In the present study, the hemolytic activity of venom from tentacles of C. nozakii and factors affecting its activity were assayed. The HUso （defined as the amount of protein required to lyse 50% of erythrocytes） of the venom against dove and chicken erythrocytes was 34 and 59 gg/mL, respectively. Carboxylmethyl chitosan and glycerol could increase hemolytic activity at concentrations greater than 0.06% and 0.2 mol/L, respectively.展开更多
文摘Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.
基金Project(2006AA03Z115) supported by the National High-tech Research and Development Program of ChinaProject(2011CB606302-1) supported by the National Basic Research Program of ChinaProject(50774007) supported by the National Natural Science Foundation of China
文摘An innovative one-step semi-solid processing technique of A356 Al alloy,the serpentine channel pouring rheo-diecasting process （SCRC）,was explored.The mechanical properties and microstructures of the tensile samples made by the SCRC technique were tested in the as-cast and T6 heat treatment conditions.The experimental results show that the as-cast ultimate tensile strength can reach about 250MPa and the elongation is 8.6%？13.2%.The ultimate tensile strength can increase approximately 30% higher than that of the as-cast one but there is some slight sacrifice of the plasticity after T6 heat treatment.Under these experimental conditions,the semi-solid A356 Al alloy slurry with primary α1（Al） grains,which have the shape factor of 0.78？0.89 and the grain diameter of 35？45μm,can be prepared by the serpentine channel pouring process.The primary α2（Al） grains are very fine during the secondary solidification stage.Compared with the conventional HPDC process,the SCRC process can improve the microstructures and mechanical properties of the tensile test samples.The advantages of the SCRC process include easily incorporating with an existing HPDC machine,cancelling the preservation and transportation process of the semi-solid alloy slurry,and a higher cost performance.
文摘Aim To investigate the anticancer activity of two new cytotoxins from thevenom of Agkistrodon acutus. Methods The venom was isolated by FPLC column chromatography consistingof DEAE Sepharose FF and Source 30S. The cytotoxic activity on tumor cells was detected by MITmethod. Purity and molecular weight were determined by SDS-PAGE (silver staining). Their stabilitiesto temperature and pH were also detected. Results Two pure cytotoxins named ACTX-6 and ACTX-8 wereobtained. Their molecular weights are 98 kDa and 27 kDa, respectively. ACTX-6 consists of twosubunits bonded together by disulfide bonds. Conclusion ACTX-6 and ATCX-8 have highest inhibitoryactivity on lung cancer cell A549. ACTX-6 is stable to heat while ACTX-8 not. ACTX-6 is stablebetween pH 7-9 and ACTX-8 between pH 6 - 9.
基金Project(2018TP1002)supported by the Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources,ChinaProject(2019zzts994)supported by the Central South University Graduate Research and Innovation Project,China。
文摘The relationship between viscosity/yield stress and flotation rate in different chalcopyrite and lizardite concentrations was investigated by zeta potential measurements,rheological tests,flotation experiments,and DerjguinLandau-Verwey-Overbeek theoretical calculation.Results indicated that the concentration of minerals would affect the viscosity and yield stress of the slurry.That is,the viscosity/yield stress in the slurry rises accordingly when the concentration of minerals increases.The increase in viscosity/yield stress in slurry is also unfavorable to the enrichment of chalcopyrite but is conducive to the entrainment of lizardite in either single or binary system.Specifically,the flotation rate of chalcopyrite decreases while that of lizardite increases with the rise in viscosity/yield stress.
文摘Stability of Defibrase in various pH buffer solutions was investigated. Enzyme-linked immuno-sorbent assay (ELISA) and coagulating time method were used to assess antigenic stability and coagulating stability, respectively. The change of antigenic activities and coagulating activities of Defibrase in the same buffer solutions (pH 6, 7 and 8, with the exception of pH 3.6) showed similar tendency to decline with the time. Concentrated Defi-brase was relatively stable at neutral pH 6~7, more than 95% of its initial activities (100BUmL-1) was kept after a 10-day storage at 40 oC, whereas in pH 3.6 and pH 9 buffer solutions, diluted Defibrase was very labile. Addition of Triton X-100 or bovine serum albumin could effectively prevent loss of Defibrase by minimizing adsorption of De-fibrase to plastic surface (P<0.005). Concentration of Defibrase could also affect its stability in aqueous solutions.
基金Supported by the National Natural Science Foundation of China(Nos.41376004,41406152)the Key R&D program of Shandong Province(Nos.2015GSF115022,2015GSF115034)the China Postdoctoral Science Foundation(No.2013M531654)
文摘Cyanea nozakii Kishinouye （C. nozakii）, a giant cnidarian of the class Scyphomedusae, order Semaeostomeae and family Cyaneidae, is widely distributed in the East China Sea, the Yellow Sea and the Bohai Sea, and is abundant from late summer to early autumn. Venom produced by C. nozakii during mass agglomerations can contaminate seawater resulting in death of the halobios and seriously damage commercial fisheries. Swimmers and fishermen commonly suffer painful stings from this j ellyfish, resulting in local edema, tingling, breathing difficulties, depressed blood pressure and even death. Such effects arise from the complex mixture of biologically active molecules that make up jellyfish venom. In the present study, the hemolytic activity of venom from tentacles of C. nozakii and factors affecting its activity were assayed. The HUso （defined as the amount of protein required to lyse 50% of erythrocytes） of the venom against dove and chicken erythrocytes was 34 and 59 gg/mL, respectively. Carboxylmethyl chitosan and glycerol could increase hemolytic activity at concentrations greater than 0.06% and 0.2 mol/L, respectively.
