目的研究蛋清蛋白肽抗氧化作用模式。方法利用超滤技术分离蛋清蛋白木瓜蛋白酶酶解产物;采用Fenton体系、邻苯三酚自氧化体系和亚油酸自氧化体系分别测定超滤各组分清除羟自由基、超氧阴离子及抑制脂质过氧化的能力,同时测定各组分对二...目的研究蛋清蛋白肽抗氧化作用模式。方法利用超滤技术分离蛋清蛋白木瓜蛋白酶酶解产物;采用Fenton体系、邻苯三酚自氧化体系和亚油酸自氧化体系分别测定超滤各组分清除羟自由基、超氧阴离子及抑制脂质过氧化的能力,同时测定各组分对二苯代苦味肼基自由基清除能力(DPPH自由基)、还原能力及对猪胎儿成纤维细胞(porcine embryonic fibroblast,PEF)过氧化损伤的保护作用。结果超滤各组分中分子量小于3kDa组分(蛋清蛋白酶解产物-Ⅲ,egg white protein hydrolysate,EWPH-Ⅲ)占蛋清蛋白酶解产物(EWPHs)总量的50.06%。EWPH的抗氧化活性随分子量的降低而增强(P<0.05),其中EWPH-Ⅲ在浓度为5mg/mL时,对羟自由基、超氧阴离子、DPPH自由基的清除率分别为52.86%、35.05%和78.74%,对亚油酸氧化的抑制率为74.57%。在浓度为2.5mg/mL时,PEF细胞存活率达到70.06%。结论蛋清蛋白肽具有较强的抗氧化活性且分子量越小,抗氧化活性越强,可以作为氢供体、自由基稳定剂和金属离子螯合剂来抑制过氧化作用。展开更多
A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennek...A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennekam syndrome (HS) is one of them. The principal treatment for PIL is a high protein, low fat diet with medium chain triglycerides supplementation. Supportive therapy includes albumin infusion. Few publications have supported the use of octreotide to diminish protein loss and minimize hypoalbuminemia seen in PIL. There are no publications on the treatment of PIL with octreotide in patients with HS. We report two children with HS and PLE in which we used octreotide to decrease intestinal protein loss. In one patient, octreotide increased serum albumin to an acceptable level without further need for albumin infusions. The other patient responded more dramatically with near normal serum albumin levels and cessation of albumin infusions. In achieving a good response to octreotide in both patients, we add to the publications supporting the use of octreotide in PIL and suggest that octreotide should be tried in patients with PIL secondary to HS. To the best of our knowledge, this is the first case report on the use of octreotide in HS-associated PIL.展开更多
文摘目的研究蛋清蛋白肽抗氧化作用模式。方法利用超滤技术分离蛋清蛋白木瓜蛋白酶酶解产物;采用Fenton体系、邻苯三酚自氧化体系和亚油酸自氧化体系分别测定超滤各组分清除羟自由基、超氧阴离子及抑制脂质过氧化的能力,同时测定各组分对二苯代苦味肼基自由基清除能力(DPPH自由基)、还原能力及对猪胎儿成纤维细胞(porcine embryonic fibroblast,PEF)过氧化损伤的保护作用。结果超滤各组分中分子量小于3kDa组分(蛋清蛋白酶解产物-Ⅲ,egg white protein hydrolysate,EWPH-Ⅲ)占蛋清蛋白酶解产物(EWPHs)总量的50.06%。EWPH的抗氧化活性随分子量的降低而增强(P<0.05),其中EWPH-Ⅲ在浓度为5mg/mL时,对羟自由基、超氧阴离子、DPPH自由基的清除率分别为52.86%、35.05%和78.74%,对亚油酸氧化的抑制率为74.57%。在浓度为2.5mg/mL时,PEF细胞存活率达到70.06%。结论蛋清蛋白肽具有较强的抗氧化活性且分子量越小,抗氧化活性越强,可以作为氢供体、自由基稳定剂和金属离子螯合剂来抑制过氧化作用。
文摘A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennekam syndrome (HS) is one of them. The principal treatment for PIL is a high protein, low fat diet with medium chain triglycerides supplementation. Supportive therapy includes albumin infusion. Few publications have supported the use of octreotide to diminish protein loss and minimize hypoalbuminemia seen in PIL. There are no publications on the treatment of PIL with octreotide in patients with HS. We report two children with HS and PLE in which we used octreotide to decrease intestinal protein loss. In one patient, octreotide increased serum albumin to an acceptable level without further need for albumin infusions. The other patient responded more dramatically with near normal serum albumin levels and cessation of albumin infusions. In achieving a good response to octreotide in both patients, we add to the publications supporting the use of octreotide in PIL and suggest that octreotide should be tried in patients with PIL secondary to HS. To the best of our knowledge, this is the first case report on the use of octreotide in HS-associated PIL.
