Analysis of the Protein Structure and Function of HpaG_(Xoo)

[Objective] The study was to analyze the structure and function of HpaGXoo and the relationship between the two.[Method] Some related bioinformatics analysis software on internet such as NPSA,Swiss-Model,SAPS and InterPro Scan were adopted to analyze the structure and predict its function.[Result] HpaGXoo consists of 139 amino acids,and has many alpha-helical and coiled structure,no signal peptide on N-terminal and no transmembrane structure.It locates in bacterial cytoplasm.[Conclusion] The study will lay ...

毛果杨copine/BONZAI基因家族全基因组鉴定与组织表达特异性分析

利用copine蛋白保守序列进行blastx同源比对,鉴定毛果杨copine/BONZAI基因家族,构建系统进化树;通过在线网站进一步分析毛果杨copine蛋白的理化性质、结构和功能。利用数据库数据对copine/BONZAI基因在根、茎和叶等组织器官中的表达模式进行分析。结果显示,毛果杨含有3个copine/BONZAI基因,分别为PtBON1、PtBON2和PtBON3,氨基酸残基数分别为573 aa、576 aa、579 aa;相对分子质量分别为62865.57、63191.81、63980.64 kD;平均理论等电点分别为5.38、5.33、5.99。PtBONs具有明显的疏水区和亲水区,没有Coil区,N端不存在信号肽,不具有跨膜结构域,均为非分泌蛋白。PtBONs二级结构中最主要的结构元件是α-螺旋和无规则卷曲。通过STRING和KEGG预测copine蛋白参与了植物与病原体相互作用过程。本研究结果为今后研究杨树copine蛋白的细胞信号转导通路机制及温度介导的抗病调控机制等提供理论依据。