Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovabumin(OVA), while OVA it...Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovabumin(OVA), while OVA itself could not induce IgE responses to it. How- ever, TCS only works when QVA immunization is given one day after TCS immunization. Either time lag in OVA immunization, or immunization of both antigens at the same time, or OVA immunization given first, all has no effect on the induction of IgE responses to OVA. Through analysis of the antibody specificity of hybridoma clones, it indicated that specific antibodies to TCS or OVA were secreted by independent B cell clones. The IgE antibodies showed no polyreactivity to different antigens.展开更多
目的:探讨过敏性哮喘患者和健康人外周血单核细胞来源树突状细胞(DC)负荷抗原后表达组成性趋化因子受体(CCR7)和MHCⅡ类分子(HLA-DR)的差异,为进一步研究过敏性哮喘的发病机制及治疗提供新的思路。方法:分别从过敏性哮喘患者(哮喘组)和...目的:探讨过敏性哮喘患者和健康人外周血单核细胞来源树突状细胞(DC)负荷抗原后表达组成性趋化因子受体(CCR7)和MHCⅡ类分子(HLA-DR)的差异,为进一步研究过敏性哮喘的发病机制及治疗提供新的思路。方法:分别从过敏性哮喘患者(哮喘组)和健康人(对照组)外周血分离单个核细胞并获取贴壁细胞,经重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和重组人白细胞介素-4(rhIL-4)体外培养为未成熟DC,加入Der p 1使其负荷抗原;观察培养的DC的形态变化,并用流式细胞仪检测两组负荷抗原的DC表面趋化因子受体(CCR7)及MHCⅡ类分子(HLA-DR)的表达。结果:与对照组相比,哮喘组负荷抗原的DC表达较高水平CCR7(P<0.01)及HLA-DR(P<0.01);两组CCR7和HLA-DR均呈正相关(哮喘组r=0.73,P<0.05;对照组r=0.76,P<0.05)。结论:由于过敏性哮喘患者负荷抗原的DC与健康人相比在趋化因子受体及成熟标志物表达方面存在明显差异,从而趋化因子反应性及成熟情况存在差异,后者成为哮喘发生的重要机制之一。展开更多
文摘Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovabumin(OVA), while OVA itself could not induce IgE responses to it. How- ever, TCS only works when QVA immunization is given one day after TCS immunization. Either time lag in OVA immunization, or immunization of both antigens at the same time, or OVA immunization given first, all has no effect on the induction of IgE responses to OVA. Through analysis of the antibody specificity of hybridoma clones, it indicated that specific antibodies to TCS or OVA were secreted by independent B cell clones. The IgE antibodies showed no polyreactivity to different antigens.
文摘目的:探讨过敏性哮喘患者和健康人外周血单核细胞来源树突状细胞(DC)负荷抗原后表达组成性趋化因子受体(CCR7)和MHCⅡ类分子(HLA-DR)的差异,为进一步研究过敏性哮喘的发病机制及治疗提供新的思路。方法:分别从过敏性哮喘患者(哮喘组)和健康人(对照组)外周血分离单个核细胞并获取贴壁细胞,经重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和重组人白细胞介素-4(rhIL-4)体外培养为未成熟DC,加入Der p 1使其负荷抗原;观察培养的DC的形态变化,并用流式细胞仪检测两组负荷抗原的DC表面趋化因子受体(CCR7)及MHCⅡ类分子(HLA-DR)的表达。结果:与对照组相比,哮喘组负荷抗原的DC表达较高水平CCR7(P<0.01)及HLA-DR(P<0.01);两组CCR7和HLA-DR均呈正相关(哮喘组r=0.73,P<0.05;对照组r=0.76,P<0.05)。结论:由于过敏性哮喘患者负荷抗原的DC与健康人相比在趋化因子受体及成熟标志物表达方面存在明显差异,从而趋化因子反应性及成熟情况存在差异,后者成为哮喘发生的重要机制之一。
