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贵州剑河白黑香猪的亲缘关系 被引量:2
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作者 刘若余 刘培琼 张启林 《山地农业生物学报》 1998年第6期337-340,共4页
用聚丙烯酰胺凝胶电泳技术,测定贵州剑河白、黑香猪血清转铁蛋白(Tf)、前白蛋白(Pa)、后白蛋白(Po)的遗传多态性,计算其基因频率。同时,利用其他学者的研究结果对贵州剑河白、黑香猪与湖南、江西等省的部分地方猪种间的... 用聚丙烯酰胺凝胶电泳技术,测定贵州剑河白、黑香猪血清转铁蛋白(Tf)、前白蛋白(Pa)、后白蛋白(Po)的遗传多态性,计算其基因频率。同时,利用其他学者的研究结果对贵州剑河白、黑香猪与湖南、江西等省的部分地方猪种间的遗传距离进行估算,利用类平均聚类法对它们之间的亲缘关系进行研究;并绘制了类平均聚类图。结果表明,贵州剑河白香猪与黑香猪的遗传距离最近,可以认为是同一品种而两个不同毛色的品系或亚群;贵州剑河白、黑香猪与湖南的湘西黑猪间遗传距离较小(均在0035以下)亲缘关系较近。 展开更多
关键词 香猪 蛋白态性 遗传距离 聚类分析 亲缘关系
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新疆南部部分绵羊品种血液遗传标记特征及聚类分析 被引量:3
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作者 李海 饶丽娟 +2 位作者 张伟信 程良润 李小武 《新疆农业科学》 CAS CSCD 2001年第z1期41-43,共3页
利用血液蛋白多态性标记新疆南部三种绵羊遗传结构 ,证实了它们相互间的遗传差异。结果表明 :Tf位点 ,多浪羊和卡拉库尔羊具有A、B、C等显性基因控制 ,巴音布鲁克羊还检测出D基因。Hb位点 ,多浪羊和卡拉库尔羊具有A、B等显性基因控制 ,... 利用血液蛋白多态性标记新疆南部三种绵羊遗传结构 ,证实了它们相互间的遗传差异。结果表明 :Tf位点 ,多浪羊和卡拉库尔羊具有A、B、C等显性基因控制 ,巴音布鲁克羊还检测出D基因。Hb位点 ,多浪羊和卡拉库尔羊具有A、B等显性基因控制 ,巴音布鲁克羊还检测出C基因。以上两个位点各种显性基因频率因品种而有差异。按照Nei’s遗传距离法 ,计算了三品种间的遗传距离 ,证明新疆南部三种绵羊品种的遗传距离以多浪羊和卡拉库尔羊为最小 ,多浪羊与巴音布鲁克羊的遗传距离为最大。用类聚法分析表明 :多浪羊与卡拉库尔羊首先相聚 ,然后再与巴音布鲁克羊相聚。 展开更多
关键词 血液蛋白态性 遗传标记 三种绵羊 聚类分析
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撒坝猪日增重生化遗传标记的筛选
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作者 连林生 鲁绍雄 《云南畜牧兽医》 2000年第2期1-4,共4页
本研究采用聚丙烯酰胺凝胶电泳法对撒坝猪的前白蛋白(Pa)、转铁蛋白(Tf)、酯酶(Es)、淀粉酶(Am)和碱性磷酸酶(Akp)等5个血清蛋白(或酶)座位的多态性进行了检测 ,采用多座位广义线性模型(GLM)对血清蛋白多态性与生长速度的关系进行了分... 本研究采用聚丙烯酰胺凝胶电泳法对撒坝猪的前白蛋白(Pa)、转铁蛋白(Tf)、酯酶(Es)、淀粉酶(Am)和碱性磷酸酶(Akp)等5个血清蛋白(或酶)座位的多态性进行了检测 ,采用多座位广义线性模型(GLM)对血清蛋白多态性与生长速度的关系进行了分析。结果表明 ,所检测的5个血清蛋白(或酶)多态座位的基因频率均处于Hardy -Weinberg平衡状态 ;Pa、Es、Am和Akp4个座位对部分阶段日增重的基因型效应达到了显著水平 ,其中 ,一些基因型具有显著提高或降低某一阶段日增重的效应 ,显示了血清蛋白多态性用作猪生长速度的遗传标记以进行标记辅助选择的可能性。同时 。 展开更多
关键词 撒坝猪 血清蛋白态性 生长 遗传标记
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Study on Polymorphisms in the Blood Protein of Tibetan Mastiff 被引量:5
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作者 兰小平 郭宪 +2 位作者 陈永昌 鄢珣 崔泰保 《Agricultural Science & Technology》 CAS 2009年第4期50-54,共5页
[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff vari... [ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus(Tf, Po, Sα2, Hb, AIb, Pr and Amy)were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei's average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation. 展开更多
关键词 Tibetan Mastiff Blood protein polymorphism Effective number of alleles Ners average expected heterozygosity
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The Effects of Apolipoprotein E Polymorphism on Serum Lipids, Lipoproteins and Apolipoproteins Variation 被引量:3
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作者 潘闽 朱健华 +2 位作者 袁瑾 王惠民 刘志华 《Journal of Nanjing Medical University》 2003年第4期196-200,共5页
Objective: To study the effects of Apolipoprotein E (ApoE) polymorphism onserum levels of lipids, lipoproteins and apolipoproteins. Methods: Fragments of ApoE gene forthex-on containing codon 112 and 158 polymorphic l... Objective: To study the effects of Apolipoprotein E (ApoE) polymorphism onserum levels of lipids, lipoproteins and apolipoproteins. Methods: Fragments of ApoE gene forthex-on containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested untilCfo I endonuclease. Genotypes and alleles frequencies of 168 healthy persons in Jiangsu area werecalculated. The effects of ApoE genotypes and alleles on serum lipids, lipoproteins andapolipoproteins variation were analyzed. Results: The effects of ApoE alleles on total cholesterol(TC), law density lipoprotein-cholesterol (LDL-C), ApoB was: along a decreasing gradientε_4>ε_3>ε_2. The effect of ε_4 allele was to increase serum levels of TC, LDL-C and ApoB, andthe ε_2 allele had an effect opposite to that of ε_4 allele. Conclusion: ApoE polymorphism is anindependent genetic factor on individual serum levels of lipids and apolipoproteins. 展开更多
关键词 Apolipoprotein E POLYMORPHISM polymerase chain reaction restrictionfragment length polymorphism
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Polymorphism Detection of the Twelfth Exon of Equine MxA Gene 被引量:1
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作者 菊林花 金花 呼都特 《Agricultural Science & Technology》 CAS 2009年第1期85-88,共4页
[Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products w... [Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism (RFLP) to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. [Result] There were three genotypes (AA, AB and BB) in the 12^th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutated from G to C, correspondingly changing the 562^th amino acid of the coding region of MxA protein from tryptophan to cysteine; the specific sequence of the PCR products amplified by mismatch PCR-RFLP was consistent with the analysis results of RFLP. [ Conclusion] The mismatch PCR-RFLP was an easy method with accurate results to detect the polymorphism of the 12^th exon of equine MxA gene. 展开更多
关键词 HORSE MxA gene POLYMORPHISM
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Impact of lipoprotein lipase gene polymorphisms on ulcerative colitis 被引量:3
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作者 Toshihito Kosaka Junji Yoshino +11 位作者 Kazuo Inui Takao Wakabayashi Kazumu Okushima Takashi Kobayashi Hironao Miyoshi Yuta Nakamura Shigekazu Hayashi Taizou Shiraishi Masatoshi Watanabe Takayuki Yamamoto Ai Nakahara Takahiko Katoh 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第39期6325-6330,共6页
AIM: To examine the influence of lipoprotein lipase (LPL) gene polymorphism in ulcerative colitis (UC) patients.METHODS: Peripheral blood was obtained from 131 patients with UC and 106 healthy controls for DNA e... AIM: To examine the influence of lipoprotein lipase (LPL) gene polymorphism in ulcerative colitis (UC) patients.METHODS: Peripheral blood was obtained from 131 patients with UC and 106 healthy controls for DNA extraction. We determined LPL gene polymorphisms affecting the enzyme at Ser447stop, as well as Hind Ⅲ and Pvu Ⅱ polymorphisms using PCR techniques. PCR products were characterized by PCR-RFLP and direct sequencing. Polymorphisms were examined for association with clinical features in UC patients. Genotype frequencies for LPL polymorphisms were also compared between UC patients and controls.RESULTS: In patients with onset at age 20 years or younger, C/G and G/G genotypes for Ser447stop polymorphism were more prevalent than C/C genotype (OR = 3.13, 95% CI = 0.95-10.33). Patients with H^+/- or H^-/- genotype for Hind Ⅲ polymorphism also were more nu merous than those with H^+/+ genotype (OR = 2.51, 95% CI = 0.85-7.45). In the group with H^+/+ genotype for Hind Ⅲ polymorphism, more patients had serum triglyceride concentrations over 150 mg/dL than patients with H^+/- or H^- genotype (P 〈 0.01, OR = 6.46, 95% CI = 1.39-30.12). Hypertriglycemia was also more prevalent in patients with P^+/+ genotypes for Pvu Ⅱ polymorphism (P 〈 0.05, OR = 3.0, 95% CI = 1.06-8.50). Genotype frequency for LPL polymorphism did not differ significantly between UC patients and controls. 展开更多
关键词 Ulcerative colitis Lipoprotein lipase Lipid metabolism TRIGLYCERIDE
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THE ASSOCIATION OF POLYMORPHISMS AT A VNTR LOCUS 3’TO THE APOLIPOPROTEIN B GENE WITH CORONARY HEART DISEASE IN CHINESE POPULATION 被引量:4
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作者 叶平 陈保生 王士雯 《Chinese Medical Sciences Journal》 CAS CSCD 1995年第2期63-69,共7页
The polymorphisms of variable number of tandem repeats (VNTR) 3’to the apolipoprotein B (apo B) gene were investigated using polymerase chain reaction (PCR) in a sample of 103 patients with documented coronary heart ... The polymorphisms of variable number of tandem repeats (VNTR) 3’to the apolipoprotein B (apo B) gene were investigated using polymerase chain reaction (PCR) in a sample of 103 patients with documented coronary heart disease (CHD) and 100 healthy individuals selected from Chinese Han nationality.Twelve segregating alleles (3’β29 -51) were observed in the pooled total of 203 subjects. The most common allele was 3’β 37. followed by 3’β39 with frequencies of 0. 362 and 0. 296, respectively. This model of allele distribution was coincident with the results form different ethnic groups, but the relative frequencies of alleles were different. In comparison with the allele frequencies between the patients and controls,alleles bigger than 3’β39 (3’VNTR-B) were significantly more common among the patients than among the controls (P<0. 001). Moreover. in the CHD group patients with plasma levels of TC≥3.88 mmol/L,LDL-C≥2. 59 mmol/L and HDL-C<l. 16mmol/L had significantly higher frequencies of 3’ VNTR-B allele (P<0. 01). Therefore,it is suggested that 3’ VNTR-B allele might be involved in the development of coronary atherosclerosis, presumably through their influences on lipid metabolism.This study supported by “8. 5” grant from Ministry of PublicHealth. 展开更多
关键词 apolipoprotein B gene polymorphisms coronary heart disease
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POLYMORPHISMS OF THE HUMAN LIPOPROTEIN LIPASE GENE:POSSIBLE ASSOCIATION WITH LIPID LEVELS IN PATIENTS WITH CORONARY HEART DISEASE IN BEIJING AREA 被引量:7
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作者 叶平 裴兰 王士雯 《Chinese Medical Sciences Journal》 CAS CSCD 1996年第3期157-161,共5页
The polymorphisms(Pvu Ⅱand Hind Ⅲ) on the lipoprotein lipase(LPL) gene locus was investigated in a sample of 100 patients surviving previous myocardial infarction and 100 age matched healthy individuals selected fro... The polymorphisms(Pvu Ⅱand Hind Ⅲ) on the lipoprotein lipase(LPL) gene locus was investigated in a sample of 100 patients surviving previous myocardial infarction and 100 age matched healthy individuals selected from Han Chinese of Beijing area.In patient group a strong association was found between H+allele of Hind Ⅲ polymorphism and raised TG levels(P<0.01).In control group P-P-genotype was observed to be associated with higher TG levels compared with P+P genotype of Pvu Ⅱ polymorphism(P<0.05).Combination of H+H+ genotype with P-P-genotype showed the highest TG levels among all nine kinds of genotype combinations in patient group(P<0.01).However,comparison of distribution of alleles and genotypes of these polymorphisms between patient group and control group demonstrated no significant difference. Our data suggest that the polymorphisms at the LPL gene,as the linkage markers with an aetiologic mutation at or around LPL gene,may constitute one of the genetic determinants for the population variation in plasma TG levels,as well as for the common dyslipidemia in Chinese population. 展开更多
关键词 restriction fragment length polymorphism coronary heart disease lipoprotein lipase
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No association of the matrix metalloproteinase 1 promoter polymorphism with susceptibility to esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma in northern China 被引量:7
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作者 XiaJin GangKuang +7 位作者 Li-ZhenWei YanLi RuiWang WeiGuo NaWang Shu-MeiFang Zhi-FengChen Jian-HuiZhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第16期2385-2389,共5页
AIM: To investigate association of the 2Gor1Gsingle nudeotide polymorphism (SNP) in matrix metalloproteinase 1 (MMP1) promoter with susceptibility to esophageal squam-ous cell carcinoma (ESCC) and gastric cardiac aden... AIM: To investigate association of the 2Gor1Gsingle nudeotide polymorphism (SNP) in matrix metalloproteinase 1 (MMP1) promoter with susceptibility to esophageal squam-ous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in a population of North China. METHODS: MMP1 promoter SNP was genotyped by polymerase-chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis in 417 cancer patients (234 ESCC and 183 GCA) and 350 healthy controls. RESULTS: The genotype frequencies of the MMP1 promoter SNP in healthy controls were 55.4% (2G/2G), 30% (1G/2G) and 14.6% (1G/1G), respectively. The genotype and allelotype distribution in ESCC and GCA patients was not significantly different from that in healthy controls (all lvalues were above 0.05). Compared with the 1G/1Ggenotype, neither the 2G/2Gnor in combination with the 1G/2G genotype significantly modified the risk of developing ESCC and GCA, the adjusted odds ratio was 1.28 (95%CI = 0.78-2.09), 1.23 (95%CI = 0.38-2.05) in ESCC and 1.39 (95%CI = 0.80-2.41), 1.34 (95%CI = 0.74-2.40) in GCA, respectively. When stratified by smoking status and family history of upper gastrointestinal cancer, the 2G/2Ggenotype alone or in combination with the 1G/2G genotype also did not show any significant influence on the risk of ESCC and GCA development. In addition, influence of the MMP1 SNP on lymphatic metastasis in ESCC and GCA was also not obs-erved. CONCLUSION: The 2Gor 1GSNP in the MMP1 promoter might not modify the risk of ESCC and GCA development and might not be used as a stratification marker to predict the potential of lymphatic metastasis in these two tumor types. 展开更多
关键词 SNP ESCC GCA
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No association between phosphatase and tensin homolog genetic polymorphisms and colon cancer 被引量:2
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作者 Lynette S Phillips Cheryl L Thompson +4 位作者 Alona Merkulova Sarah J Plummer Thomas C Tucker Graham Case Li Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第30期3771-3775,共5页
AIM: To investigate the association between single nucleotide polymorphisms (SNPs) in the phosphatase and tensin homolog (PTEN) tumor suppressor gene and risk of colon cancer. METHODS: We utilized a population-based c... AIM: To investigate the association between single nucleotide polymorphisms (SNPs) in the phosphatase and tensin homolog (PTEN) tumor suppressor gene and risk of colon cancer. METHODS: We utilized a population-based casecontrol study of incident colon cancer individuals (n= 421) and controls (n = 483) aged ≥ 30 years to conduct a comprehensive tagSNP association analysis of the PTEN gene. RESULTS: None of the PTEN SNPs were statistically significantly associated with colon cancer when controlled for age, gender, and race, or when additionally adjusted for other known risk factors (P > 0.05). Haplotype analyses similarly showed no association between the PTEN gene and colon cancer. CONCLUSION: Our study does not support PTEN as a colon cancer susceptibility gene. 展开更多
关键词 Colon cancer Phosphatase and tensinhomolog Candidate gene Genetic polymorphisms Single nucleotide polymorphism association
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Several factors including ITPA polymorphism influence ribavirin-induced anemia in chronic hepatitis C 被引量:1
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作者 Akihito Tsubota Noritomo Shimada +10 位作者 Hiroshi Abe Kai Yoshizawa Rie Agata Yoko Yumoto Makiko Ika Yoshihisa Namiki Keisuke Nagatsuma Hiroshi Matsudaira Kiyotaka Fujise Norio Tada Yoshio Aizawa 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第41期5879-5888,共10页
AIM: To construct formulae for predicting the likelihood of ribavirin-induced anemia in pegylated interferon plus ribavirin for chronic hepatitis C. METHODS: Five hundred and sixty-one Japanese patients with hepatit... AIM: To construct formulae for predicting the likelihood of ribavirin-induced anemia in pegylated interferon plus ribavirin for chronic hepatitis C. METHODS: Five hundred and sixty-one Japanese patients with hepatitis C virus genotype lb who had received combination treatment were enrolled and as- signed randomly to the derivation and confirmatory groups. Single nucleotide polymorphisms at or nearby ITPA were genotyped by real-time detection poly- merase chain reaction. Factors influencing significant anemia (hemoglobin concentration 〈 10.0 g/dL at week 4 of treatment) and significant hemoglobin decline (declining concentrations 〉 3.0 g/dL at week 4) were analyzed using multiple regression analyses. Prediction formulae were constructed by significantly independent factors. RESULTS: Multivariate analysis for the derivation group identified four independent factors associated with significant hemoglobin decline: hemoglobin decline at week 2 [P = 3.29× 10^4, odds ratio (OR) = 7.54 (g/dL)], estimated glomerular filtration rate [P = 2.16× 10^4, OR = 0.962 (ml/min/1.73 m2)], rs1127354 (P = 5.75 × 10^4, OR = 10.94) and baseline hemoglobin [P = 7.86 × 10^4, OR = 1.50 (g/alL)]. Using the model constructed by these factors, positive and negative predictive values and predictive accuracy were 79.8%, 88.8% and 86.2%, respectively. For the confirmatory group, they were 83.3%, 91.0% and 88.3%. These factors were closely correlated with significant anemia. However, the model could not be constructed, because no patients with rs1127354 minor genotype CA/AA had significant anemia. CONCLUSION: Reliable formulae for predicting the likelihood of ribavirin-induced anemia were constructed. Such modeling may be useful in developing individual tailoring and optimization of ribavirin dosage. 展开更多
关键词 Chronic hepatitis C virus infection Ribavi-rin Pegylated interferon Prediction model Hemolyticanemia Single nucleotide polymorphism
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β-FIBRINOGEN PROMOTER -455 G/A(HaeIII)POLYMORPHISM PREDICTION OF PLASMA FIBRINOGEN BUT NOT OF ISCHEMIC CEREBROVASCULAR DISEASE 被引量:5
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作者 毕胜 王德生 +2 位作者 李国忠 温世荣 潘尚哈 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第1期1-5,共5页
Objective The -455 G/A(HaeIII)polymorphism of β-fibrinogen gene influences levels of plasma fibrinogen. We further investigated whether it influences the risk of ischemic cerebrovascular disease. Methods We accumulat... Objective The -455 G/A(HaeIII)polymorphism of β-fibrinogen gene influences levels of plasma fibrinogen. We further investigated whether it influences the risk of ischemic cerebrovascular disease. Methods We accumulated 134 acute ischemic cerebrovascular disease(ICVD)cases and compared their -455 G/A status with a control group(n = 166). The β-fibrinogen gene -455 G/A polymorphism was analyzed for all subjects by PCR-RFLP with the restrictive enzyme HaeIII. Results Plasma fibrinogen was higher in AA homozygous participants(341 mg/dL)than in partici-pants carrying the G allele: GA(290 mg/dL), GG(298 mg/dL)in the control group. Plasma fibrinogen was also higher in AA homozygous patients(353 mg/dL)than in cases carrying the G allele: GA(287 mg/dL), GG(302 mg/dL)in the ICVD group. However, there was no significant association between β-fibrinogen gene -455 G/A polymorphism and ICVD group. Conclusions Although a small effect cannot be excluded, β-fibrinogen gene -455 G/A polymor-phism is an independent predictor of plasma fibrinogen, but not of ischemic cerebrovascular disease. 展开更多
关键词 ischemic cerebrovascular disease β-fibrinogen gene plasma fibrinogen
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Dynamic changes and surveillance function of prion protein expression in gastric cancer drug resistance 被引量:9
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作者 Ji-Heng Wang Jing-Ping Du +5 位作者 Ying-Hai Zhang Xiao-Jun Zhao Ru-Ying Fan Zhi-Hong Wang Zi-Tao Wu Ying Han 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第35期3986-3993,共8页
AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A ... AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established,and the expression of PrPc,Bcl-2 and Bax was detected in these cells.Apoptosis was determined using Annexin V staining.Western blotting and immunohisto-chemistry were performed to detect the expression of PrPc in patients receiving chemotherapy and to explore the role of PrPc expression in predicting the chemosensitivity and the outcome of gastric cancer patients receiving chemotherapy.Follow-up was performed for 2 years.RESULTS:PrPc expression was increased with the increase in drug resistance.Bcl-2,together with PrPc,increased the level of anti-apoptosis of cancer cells.Increased PrPc expression predicted the enhanced level of anti-apoptosis and resistance to anticancer drugs.PrPc expression could be used as a marker for predicting the efficacy of chemotherapy and the prognosis of gastric cancer.Increased PrPc expression predicted both poor chemosensitivity and a low 2-year survival rate.Contrarily,low PrPc expression predicted favorable chemosensitivity and a relatively high 2-year survival rate.CONCLUSION:PrPc expression is associated with histological types and differentiation of gastric cancer cells;The PrPc expression level might be a valuable marker in predicting the efficacy of chemotherapy and the prognosis of gastric cancer patients receiving chemotherapy. 展开更多
关键词 Prion protein Gastric cancer Drug resistance CHEMOTHERAPY APOPTOSIS
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Identification of K-Casein Polymorphism in Bali Cattle
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作者 Muh Affan Mu'in Andoyo Supriyantono 《Journal of Agricultural Science and Technology(A)》 2012年第3期397-401,共5页
The present study was conducted to identify K-casein (Kappa-casein) polymorphism in the Bali cattle (Bos sondaicus). A 780 bp specific fragment of K-casein gene spanning from the forth exon region (517 bp) to fo... The present study was conducted to identify K-casein (Kappa-casein) polymorphism in the Bali cattle (Bos sondaicus). A 780 bp specific fragment of K-casein gene spanning from the forth exon region (517 bp) to forth intron (263 bp) was amplified from 60 individuals from the Bali Cattle Breeding Centre in Jembrana regency, Bali province. PCR-RFLP analysis with HindlII indicated that only the BB genotype was found at locus I, while with PstI indicated that two genotypes AA and AB were found at the k-casein locus If. The frequencies of A and B alleles at locus II were 0.91 and 0.09, respectively. Mutations in locus II did not change the amino acid coding (silent mutation). At locus I, comparison with allele frequencies in other cattle breeds indicates that frequencies in Bali cattle (Bos sondaicus) are significantly different from those observed in Bos taurus and Bos indicus breeds. 展开更多
关键词 POLYMORPHISM k-casein PCR-RFLP Bali cattle genotype.
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Coalition of DNA polymorphisms of ApoB and ApoAI genes is related with coronary artery disease in Kazaks 被引量:4
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作者 Gang Huang Hua Zhong +3 位作者 He-Man Re Hong-Wei Mao Qiang Niu Ye-Hong Chi 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2012年第1期33-37,共5页
Objective To explore the relationship between polymorphisms of XbaI and MspI loci of apolipoprotein B (ApoB) gene and -75 bp,+83 bp loci of apolipoprotein AI (ApoAI) gene and coronary heart disease (CHD) in Kaz... Objective To explore the relationship between polymorphisms of XbaI and MspI loci of apolipoprotein B (ApoB) gene and -75 bp,+83 bp loci of apolipoprotein AI (ApoAI) gene and coronary heart disease (CHD) in Kazaks of Xinjiang Uyghur Autonomous Region,China.Methods These loci were analyzed by PCR-restriction fragment length polymorphism (PCR-PFLP).Two hundred and five patients with CHD and two hundred and thirty six controls were involved.Results There were significant distinctions among low-density lipoprotein cholesterol (LDL-C),triglyceride (TG) and the ApoAI/ApoB ratio between the two groups,but no significant distinction among the polymorphism frequencies of the four sites between the two groups.The polymorphism coalition frequency of X-/Ms++/M1+-/M2++ (named Coalition 11) was significantly higher in CHD compared to the control group (14.6% vs.7.2%,P < 0.05).The level of total cholesterol (TC) in Coalition 1 1 was significantly higher and the level of the ApoAI/ApoB ratio in Coalition 11 was significantly lower than Coalition 1~10 in CHD patients.The level of the ApoAI/ApoB ratio of Coalition 11 was significantly lower than the Coalition 1~10 in control group.The levels of ApoAI/ApoB ratio of Coalition 3 were significantly higher compared to Coalition 11 in the two groups,respectively.The level of LDL-C of Coalition 3 was significantly lower than in the Coalition 11 in control group.The level of TC of Coalition 5 was significantly higher than Coalition 3 in the CHD group.The level of the ApoAI/ApoB ratio of Coalition 5 was significantly lower than in Coalition 3 or Coalition 1~10 of the two groups,respectively.The level of LDL-C of Coalition 5 was significantly higher than in Coalition 3 in control group.The ratio of ApoAI/ApoB was negatively related to TC,LDL-C and was positively related to HDL-C,both in CHD and control groups.Conclusion Coalition 11 of the 4 loci polymorphisms of the ApoB and ApoAI genes was correlated with CHD in Kazaks,and perhaps the ratio of ApoAI/ApoB was the most diagnostic parameter related with CHD among all lipid parameters.CHD may also be associated with Coalition 5,and,perhaps,Coalition 3 may have been confirmed as a protection factor against CHD,if more samples were enrolled. 展开更多
关键词 APOB APOAI Gene polymorphism Coronary heart disease Kazaks
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Study of apolipoprotein E genetic polymorphism in patients with atherosclerotic cerebral infarction 被引量:2
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作者 罗本燕 陈智 +2 位作者 陈峰 李霞 潘小平 《Journal of Zhejiang University Science》 CSCD 2003年第6期749-752,共4页
Objective: To explore the frequency and significance of ApoE gene polymorphisms in Chinese patients with atherosclerotic cerebral infarction (ACI). Methods: Polymerase chain reaction and gene sequencing, single nucleo... Objective: To explore the frequency and significance of ApoE gene polymorphisms in Chinese patients with atherosclerotic cerebral infarction (ACI). Methods: Polymerase chain reaction and gene sequencing, single nucleotide polymorphisms of ApoE gene were used to analyze 33 cases of patients with ACI and 35 controls. Results: The frequencies of ApoE gene single nucleotide polymorphisms 465C/G, 462C/G and 451delC in the ACI group were significantly higher than those in the control group (P<0.05). The prevalence of polymorphism 486G/T in the control group was significantly higher than that in the ACI group ( P = 0.011) . Conclusions: 465C/G,462C/G and 451delC polymorphisms might be associated with ACI.486GT allele might have protective effect on the pathogenesis of ACI. 展开更多
关键词 Cerebral infarction ATHEROSCLEROTIC GENETICS ApoE allele Single nucleotide polymorphism
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Comparative Studies of the Biochemical Parameters of the Leaves and Seeds of Moringa oleifera
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作者 Abayomi Ezekiel Folorunso Kemi Feyisayo Akinwunmi Raphael Emuebie Okonji 《Journal of Agricultural Science and Technology(B)》 2012年第6期671-677,共7页
Comparative studies of the biochemical parameters of the leaves and seeds of Moringa oleifera was carried out to provide additional information on the nutritional status of the leaf and seed of M, oleifera. The vegeta... Comparative studies of the biochemical parameters of the leaves and seeds of Moringa oleifera was carried out to provide additional information on the nutritional status of the leaf and seed of M, oleifera. The vegetative and floral characters of the plants collected were examined, floral diagram and formular were recorded. Protein variation in the dry seed and leaf is an indication of protein polymorphism and this depicts the genetic divergence in the leaf and seed ofM. oleifera. Protein bands at 0.4, 1.1, 3.9 and 5.8 are taxonomically distinct, thus diagnostically separated the dry seed from other samples. Proximate analysis shows that the dry samples are more nutritious than the wet samples. 展开更多
关键词 Vegetative character floral character protein band proximate analysis Moringa oleifera.
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CORRELATION BETWEEN THE POLYMORPHISM OF GLYCOPROTEIN Ⅰa GENE AND ACUTE CORONARY SYNDROME
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作者 赵永辉 王燕妮 祝家庆 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第1期13-18,共6页
Objective The platelet membrane glycoprotein (GP)Ⅰa/Ⅱa plays a major part as a primary colla-gen receptor in platelet function. Previous studies indicated that variations of GPⅠa/Ⅱa density and function are associ... Objective The platelet membrane glycoprotein (GP)Ⅰa/Ⅱa plays a major part as a primary colla-gen receptor in platelet function. Previous studies indicated that variations of GPⅠa/Ⅱa density and function are associated with the 807 C/T polymorphism of GPⅠa gene in American and Spanish Cau-casian populations. This study investigated the correlation between acute coronary syndrome (ACS) and 807 C/T dimorphism of GPⅠa gene in Chinese of Han ethnicity. Methods A case-control study was carried out, including 75 patients with either acute myocar-dial infarction (AMI) or unstable angina pectoris (UAP), and 65 controls with no history of coronary heart disease, thrombogenic and hemorrhagenic diseases. Genotypes of GPⅠa were checked by polymerase chain reaction-sequence specific primers(PCR-SSP)technique. Results The frequencies of both homozygotes and heterozygotes for T807 allele(TT+TC)were significantly higher in patients with AMI than in controls(62.16% vs 33.85%, P < 0.01; odds ratio 3.21). The prevalence of (TT+TC) genotypes was also markedly higher in patients with UAP than in controls(65.79% vs 33.85%, P < 0.005; odds ratio 3.76). There was significant difference in the distribution of(TT+TC)genotypes not only between all patients and controls(64.00% vs 33.85%, P <0.005; odds ratio 3.47)but also between the two subgroups aged < 60 years(70.00% vs 38.24%, P <0.005; odds ratio 3.77). However, there was no significant difference in the distribution of(TT+TC)genotypes between patients with AMI and with UAP. Platelet GPⅠa T807 allele remained significantly associated with AMI and UAP by multiple logistic regression(odds ratio 4.94). Conclusion This study suggests a strong association between presence of GPⅠa T807 allele and ACS. T807 allele can be a marker of genetic susceptibility to ACS. 展开更多
关键词 GLYCOPROTEIN GENE POLYMORPHISM acute coronary syndrome
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Chemometrics of differentially expressed proteins from colorectal cancer patients 被引量:1
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作者 Lay-Chin Yeoh Saravanan Dharmaraj +2 位作者 Boon-Hui Gooi Manjit Singh Lay-Harn Gam 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第16期2096-2103,共8页
AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.METHODS:A Proteomic approach was used to identify the differential... AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.METHODS:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal tissues.The proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,respectively.Chemometrics,namely principal component analysis (PCA) and linear discriminant analysis (LDA),were used to assess the usefulness of these proteins for identifying the cancerous state of tissues.RESULTS:Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB extracts.Based on the protein spots intensity on 2D-gel images,PCA by applying an eigenvalue > 1 was successfully used to reduce the number of principal components (PCs) into 12 and seven PCs for Tris and TLB extracts,respectively,and subsequently six PCs,respectively from both the extracts were used for LDA.The LDA classification for Tris extract showed 82.7% of original samples were correctly classified,whereas 82.7% were correctly classified for the cross-validated samples.The LDA for TLB extract showed that 78.8% of original samples and 71.2% of the cross-validated samples were correctly classified.CONCLUSION:The classification of CRC tissues by PCA and LDA provided a promising distinction between normal and cancer types.These methods can possibly be used for identification of potential biomarkers among the differentially expressed proteins identified. 展开更多
关键词 Colorectal cancer PROTEOMICS Marker protein Principal component analysis Linear discriminant analysis
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