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鹌鹑脏器、肌肉蛋白质多型座位电泳技术 被引量:1
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作者 郑惠玲 常洪 +3 位作者 Akio Sano 刘小林 李相运 任战军 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2001年第3期79-83,共5页
将应用于家畜血液蛋白质多型座位的电泳技术加以改进,成功地应用于鹌鹑脏器、肌肉蛋白质多型座位的检测,使可检测的鹌鹑蛋白质多型座位数从国内报道的3个增至32个。详细介绍了检测32个鹌鹑脏器、肌肉蛋白质多型座位的淀粉凝胶电... 将应用于家畜血液蛋白质多型座位的电泳技术加以改进,成功地应用于鹌鹑脏器、肌肉蛋白质多型座位的检测,使可检测的鹌鹑蛋白质多型座位数从国内报道的3个增至32个。详细介绍了检测32个鹌鹑脏器、肌肉蛋白质多型座位的淀粉凝胶电泳技术,为客观评价鹌鹑遗传资源提供了科学的研究方法。 展开更多
关键词 鹌鹑 蛋白质多型 淀粉凝胶电泳 脏器 肌肉 基因座位
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合作猪血液蛋白多型性研究 被引量:4
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作者 李相运 任战军 +1 位作者 常洪 黄汉军 《西北农业大学学报》 CSCD 北大核心 2000年第4期62-64,共3页
以简单随机抽样法 ,应用淀粉凝胶电泳技术对 55头合作猪 1 3个血液蛋白座位的多型性进行了分析。结果表明 :合作猪在运铁蛋白 ( Tf)、淀粉酶 ( Am)、6-磷酸葡萄糖酸脱氢酶 ( 6PGD)及碳酸酐酶 ( Ca) 4个座位表现多型 ,分别受 4,4,2 ,3个... 以简单随机抽样法 ,应用淀粉凝胶电泳技术对 55头合作猪 1 3个血液蛋白座位的多型性进行了分析。结果表明 :合作猪在运铁蛋白 ( Tf)、淀粉酶 ( Am)、6-磷酸葡萄糖酸脱氢酶 ( 6PGD)及碳酸酐酶 ( Ca) 4个座位表现多型 ,分别受 4,4,2 ,3个共显性等位基因支配 ,其中 Ca C基因为首次发现。其余 9个座位即 Cp、Hp、CEs、Pa、Es D、PHI、G6PD、PGM、MDH未发现多型。 展开更多
关键词 合作猪 血液蛋白质多型 群体遗传学 遗传标记
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其它
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《中国农业文摘(畜牧)》 2002年第3期49-49,共1页
S837.2 20021348鹌鹑脏器、肌肉蛋白质多型座位电泳技术/郑惠玲(西北农林科技大学畜牧兽医学院 712100),常洪,Akio Sano…//西北农林科技大学学报(自然科学版).-2001,29(3).
关键词 蛋白质多型 电泳技术 西北农林科技大学学报 产蛋性能 平均产蛋率 畜牧兽医学院 自然科学版 肌肉 座位 脏器
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Genetic polymorphisms of MAFK, encoding a small Maf protein, are associated with susceptibility to ulcerative colitis in Japan 被引量:1
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作者 Tomiyasu Arisawa Masakatsu Nakamura +10 位作者 Toshimi Otsuka Wu Jing Naoko Sakurai Hikaru Takano Tasuku Hayashi Masafumi Ota Tomoe Nomura Ranji Hayashi Takeo Shimasaki Tomomitsu Tahara Tomoyuki Shibata 《World Journal of Gastroenterology》 SCIE CAS 2017年第29期5364-5370,共7页
To investigate whether single nucleotide polymorphisms in maf protein K (MAFK), which encodes the MAFK, lead to increased susceptibility to ulcerative colitis in the Japanese population. METHODSThis case control study... To investigate whether single nucleotide polymorphisms in maf protein K (MAFK), which encodes the MAFK, lead to increased susceptibility to ulcerative colitis in the Japanese population. METHODSThis case control study examined the associations between MAFK single nucleotide polymorphisms (rs4268033 G>A, rs3735656 T>C and rs10226620 C>T) and ulcerative colitis susceptibility in 174 patients with ulcerative colitis (UC) cases, and 748 subjects without no lower abdominal symptoms, diarrhea or hematochezia (controls). In addition, as the second controls, we set 360 subjects, who have an irregular bowel movement without abnormal lower endoscopic findings (IBM controls). RESULTSThe genotype frequency of rs4268033 AA and allelic frequency of the rs4268033A allele were significantly higher in the UC cases than in both controls (P = 0.0005 and < 0.0001, P = 0.015 and 0.0027 vs controls and IBM controls, respectively). Logistic regression analysis after adjustment for age and gender showed that the rs4268033 AA and rs3735656 CC genotypes were significantly associated with susceptibility to UC development (OR = 2.63, 95%CI: 1.61-4.30, P = 0.0001 and OR = 1.81; 95%CI: 1.12-2.94, P = 0.015, respectively). Similar findings were observed by the comparison with IBM controls. In addition, the rs4268033 AA genotype was significantly associated with all phenotypes of UC except early onset. There was no significant association between rs10226620 and ulcerative colitis. CONCLUSIONOur results provide the first evidence that MAFK genetic polymorphisms are significantly associated with susceptibility to UC development. In particular, rs4268033 is closely associated with an increased risk for the development of UC. 展开更多
关键词 Maf protein K Genetic polymorphism Reactive oxygen species Ulcerative colitis Nuclear factor-erythroid 2-related factor 2
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Seed Protein Electrophoresis for Assessment of Genetic Diversity among Festuca pratensis Huds, Local Ecotypes
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作者 S. D. Stoyanova B. Boller +1 位作者 Y. K. Guteva S. A. Angelova 《Journal of Agricultural Science and Technology(B)》 2011年第8期1176-1183,共8页
Fifty two accessions of Festuca pratensis Huds. were analysed of which 18 had been collected in Switzerland and 32 in Bulgaria. SDS-PAGE of seed proteins was implemented to detect genetic variation among ecotype popul... Fifty two accessions of Festuca pratensis Huds. were analysed of which 18 had been collected in Switzerland and 32 in Bulgaria. SDS-PAGE of seed proteins was implemented to detect genetic variation among ecotype populations using a modification of UPOV method for barley. The modification concerns mainly protein extracting procedure as detailed described in the protocol. The two cultivars Preval and Cosmolit were used as standards. Cluster analyses and correspondence analyses/scatter plot were used as statistic approaches for determining genetic diversity among individual ecotypes and groups of ecotypes. Electrophoretic spectra of proteins show clear differences among local accessions in relation to their origin. In Swiss ecotypes 32 protein fragments were determined whereas in Bulgarian local populations their number was 68. Each of the two eco-groups possesses fragments that appear in all accessions of the group. The number of monomorphic bands within Bulgarian local ecotypes is four whereas their number in Swiss ecotypes is 12. Four monomorphic bands appearing in all proteinograms no difference of eco-groups was identified with Rm values of: 0.43, 0.55, 0.58, 0.82. A higher level of protein band polymorphism was proven in Bulgarian ecotypes in comparison with Swiss ecotypes. Thirty seven polymorphic bands occurred exclusively in the Bulgarian local ecotypes and had a frequency of 0.03 or higher whereas within Swiss ecotypes was detected one unique protein fragment. SDS-PAGE "fingerprinting" is suggested as a fast and easy approach to differentiate F. pratensis ecotypes by their origin as well for detection of foreign germplasm for inclusion in breeding programs. 展开更多
关键词 Festuca pratensis ELECTROPHORESIS SEED identification diversity.
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