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多菌发酵马铃薯渣生产蛋白质饲料的研究 被引量:13
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作者 祝英 王治业 +2 位作者 孙智敏 周剑平 蒲训 《中国饲料》 北大核心 2009年第9期40-43,共4页
本文分析了米曲霉、黑曲霉、绿色木霉单菌和这3种菌的最佳复配菌及与酵母菌复配的发酵液中总糖、还原糖,以及产物中粗蛋白质收率的变化,探讨其对马铃薯渣生产蛋白质饲料的影响。结果表明:(1)单菌株发酵试验结果表明,米曲霉对马铃薯渣的... 本文分析了米曲霉、黑曲霉、绿色木霉单菌和这3种菌的最佳复配菌及与酵母菌复配的发酵液中总糖、还原糖,以及产物中粗蛋白质收率的变化,探讨其对马铃薯渣生产蛋白质饲料的影响。结果表明:(1)单菌株发酵试验结果表明,米曲霉对马铃薯渣的降解效果最好,将粗蛋白质的收率提高了1.82倍。(2)3种霉菌复配发酵试验的结果表明,米曲霉与黑曲霉复配降解马铃薯渣的效果最好,可将粗蛋白质收率提高1.94倍。(3)米曲霉与黑曲霉的复配菌(1∶1)在与3种酵母菌进行二次复配的试验结果表明,降解马铃薯渣的速度和粗蛋白质的收率均有明显提高,其中与产朊假丝酵母与热带假丝酵母(1∶1)进行二次复配的效果最好,粗蛋白质收率高达10.541g/L,将粗蛋白质的收率提高到2.47倍。 展开更多
关键词 马铃薯渣 米曲霉 产朊假丝酵母 蛋白质收率 DNS法
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米曲霉、黑曲霉和绿色木霉混合发酵马铃薯废渣生产细胞蛋白的研究 被引量:2
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作者 祝英 蒲训 +3 位作者 王治业 赵小锋 孙智敏 杨鑫 《中国食品工业》 2008年第11期52-53,共2页
本文将米曲霉、黑曲霉和绿色木霉三种霉菌复配,研究复配菌对马铃薯废渣的分解能力和各复配菌之间的相互作用关系。试验结果表明:三种霉菌按照1:1复配,出现四种复配结果,其中利用马铃薯废渣转化成饲料蛋白效果最好的是黑曲霉和米曲霉的... 本文将米曲霉、黑曲霉和绿色木霉三种霉菌复配,研究复配菌对马铃薯废渣的分解能力和各复配菌之间的相互作用关系。试验结果表明:三种霉菌按照1:1复配,出现四种复配结果,其中利用马铃薯废渣转化成饲料蛋白效果最好的是黑曲霉和米曲霉的复配菌,在120h内能将马铃薯废渣完全分解,且蛋白质收率最高,达到8.292g/L,高于单菌发酵蛋白质的收率(7.775g/L),将粗蛋白质的收率提高了2.59倍。此外,通过研究复合菌和单菌发酵液中总糖和还原糖的含量变化,推断出各混合菌之间的相互作用关系:黑曲霉与绿色木霉之间是互惠共生关系;米曲霉与黑曲霉是竞争关系,而且黑曲霉在分解利用马铃薯废渣的过程中占竞争优势;绿色木霉对米曲霉利用马铃薯废渣有抑制作用;三种菌混合发酵马铃薯废渣时,黑曲霉的生长占竞争优势。 展开更多
关键词 菌体蛋白 马铃薯废渣 米曲霉 蛋白质收率
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The Differences of Physiological Characteristics and Yield of Northern Japonica Rice in Different Nitrogen Application Patterns
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作者 Yuefeng CUI Tiegang LU +5 位作者 Guocai SUN Junru WANG Xiaoqiu WU Guiyan WANG Jian WANG Wenjia HUANG 《Agricultural Science & Technology》 CAS 2016年第4期864-868,共5页
The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: pan... The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: panicle was 6 ∶3∶1, the higher amount of nitrogen improved the chlorophyll content, photosynthetic capacity, effective panicle, grain weight and yield; the medium nitrogen level had advantage under the ratio 4:3:3; nitrogen application at lategrowth stage was suitable for low or medium nitrogen level,which could increase photosynthetic capacity and optimize the yield components and increase yield; under the same ratio for basic tiller and ear fertilizer, the increase of nitrogen could improve total nitrogen uptake and protein content, decrease nitrogen physiological efficiency and milled rice and tasting, but nitrogen recovery and harvest index changes had difference; as application at late growth stage in same nitrogen rate, total nitrogen uptake and protein content increased, nitrogen physiological efficiency and milled rice and amylose content and tasting decreased, nitrogen recovery and harvest index increased under low or medium nitrogen level and decreased under high nitrogen level. Nitrogen application 210 kg/hm^2 and the ratio 4 ∶3 ∶3 of basal:tillering: panicle made the yield increase by 2.8%-11.3%, and this is the optimal mode of nitrogen fertilizer application. 展开更多
关键词 Nitrogen fertilizer Japonica rice Physiological characteristics YIELD
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Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye
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作者 李翠萍 于华华 +2 位作者 冯金华 陈晓琳 李鹏程 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第1期172-176,共5页
In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel ... In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well. 展开更多
关键词 CONCENTRATION hemolytic activity Rhopilema esculentum Kishinouye VENOM
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