RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 pred...RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyl- transferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 ?, b=43.1 ?, c=118.2 ?, β=92.1°, and diffract X-rays to 2.2 ? resolution. The VM value was determined to be 2.8 ?3/Da, indicating the presence of four protein molecules in the asymmetric unit.展开更多
A sulfonated 9,10-distyrylanthracene derivative with aggregation-induced emission (AIE) property is designed and synthesized. It shows a highly sensitive and selective fluorescence enhancement property for bovine seru...A sulfonated 9,10-distyrylanthracene derivative with aggregation-induced emission (AIE) property is designed and synthesized. It shows a highly sensitive and selective fluorescence enhancement property for bovine serum albumin (BSA) protein detection and quantification. Analysis on the interaction between the probe molecule and BSA reveals the essential role of the hydrophobic cavities of the protein folding structure.展开更多
基金Project supported by the National Project on Protein Structural and Functional Analyses from the Ministry of Education, Culture, Sports, Science, and Technology of Japan
文摘RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyl- transferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 ?, b=43.1 ?, c=118.2 ?, β=92.1°, and diffract X-rays to 2.2 ? resolution. The VM value was determined to be 2.8 ?3/Da, indicating the presence of four protein molecules in the asymmetric unit.
基金the National Basic Research Pro- gram of China (973 Program, 2013CB834702, 2009CB623605)the Natural Science Foundation of China (21074045, 21204027, 21221063)+1 种基金the Research Fund for the Doctoral Program of Higher Education of China (20120061120016)the Project of Jilin Province (20100704)
文摘A sulfonated 9,10-distyrylanthracene derivative with aggregation-induced emission (AIE) property is designed and synthesized. It shows a highly sensitive and selective fluorescence enhancement property for bovine serum albumin (BSA) protein detection and quantification. Analysis on the interaction between the probe molecule and BSA reveals the essential role of the hydrophobic cavities of the protein folding structure.
