OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell ...OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.展开更多
Nanotechnology has been widely used in the field of medicine, and it can significantly improve the bioavailability and the target efficiency of medicines. However, after administration, nanomedicines can adsorb biomol...Nanotechnology has been widely used in the field of medicine, and it can significantly improve the bioavailability and the target efficiency of medicines. However, after administration, nanomedicines can adsorb biomolecules that can influence their effects. It was reported that the adsorption of plasma proteins can change the surface properties of nanoparticles. When nanoparticles pass through cells, they may carry some cellular proteins out of cells. Currently, it is unclear whether the adsorbed proteins affect the uptake of nanoparticles in the next cell layer. To simplify this complex biological process, BSA-capped gold nanoparticles were prepared and incubated with Caco-2 cell lysate to simulate conditions of transcytosis through epithelial cells. The surface morphology of nanoparticles was examined by TEM. SRB was used to evaluate the cytotoxicity of the nanoparticles. The uptake and cellular distribution of the nanoparticles were detected by ICP-MS and CLSM. The results suggested that the adsorption of cell proteins could enhance the adhesion and uptake of gold nanoparticles. The gold nanoparticles were mainly located in lysosomes, and there were some Lysate-capped AuNPs in the mitochondria whereas no BSA-capped AuNPs appeared there.展开更多
基金supported by grants from the Medical Scientific Research Foundation and Bureau of Health Care for Senior Officials of Zhejiang Province,China (No.2007B026)the National Natural Science Foundation of China (No.0471819)
文摘OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.
基金The National Basic Research Program of China(973 program,Grant No.2015CB932100)National Natural Science Foundation of China(Grant No.81130059)
文摘Nanotechnology has been widely used in the field of medicine, and it can significantly improve the bioavailability and the target efficiency of medicines. However, after administration, nanomedicines can adsorb biomolecules that can influence their effects. It was reported that the adsorption of plasma proteins can change the surface properties of nanoparticles. When nanoparticles pass through cells, they may carry some cellular proteins out of cells. Currently, it is unclear whether the adsorbed proteins affect the uptake of nanoparticles in the next cell layer. To simplify this complex biological process, BSA-capped gold nanoparticles were prepared and incubated with Caco-2 cell lysate to simulate conditions of transcytosis through epithelial cells. The surface morphology of nanoparticles was examined by TEM. SRB was used to evaluate the cytotoxicity of the nanoparticles. The uptake and cellular distribution of the nanoparticles were detected by ICP-MS and CLSM. The results suggested that the adsorption of cell proteins could enhance the adhesion and uptake of gold nanoparticles. The gold nanoparticles were mainly located in lysosomes, and there were some Lysate-capped AuNPs in the mitochondria whereas no BSA-capped AuNPs appeared there.
