地肤子为常用中药,全四大多数地区使用藜科植物地肤[Kochia scoparia(L.)Schrad.]的胞果入药。在华东、湖南、湖北、江西等某些地区尚用同科植物藜(Chenopodium album L.)的胞果(灰菜子)。据考证,历代本草所载地肤子即现今藜科植物地肤...地肤子为常用中药,全四大多数地区使用藜科植物地肤[Kochia scoparia(L.)Schrad.]的胞果入药。在华东、湖南、湖北、江西等某些地区尚用同科植物藜(Chenopodium album L.)的胞果(灰菜子)。据考证,历代本草所载地肤子即现今藜科植物地肤的胞果,与《中国药典》1985年版一部收载品种一致。地肤子与藜的胞果(灰菜子)形态近似,但药效有别,二者不可混用。因此,我们利用聚丙烯酰胺凝胶电泳技术对它们进行了蛋白电泳。发现二者的蛋白质谱带具有显著差异,可作为地肤子及灰菜子的鉴别依据。一、材料与方法 1.实验材料:地肤[Kochia scoparia(L.)展开更多
The amide A band of protein is sensitive to the hydrogen bands of amide groups of proteins. However, it is hard to distinguish the amide A band of aqueous protein in situ directly, since it overlaps with O-H stretchin...The amide A band of protein is sensitive to the hydrogen bands of amide groups of proteins. However, it is hard to distinguish the amide A band of aqueous protein in situ directly, since it overlaps with O-H stretching vibration of water. In this work, we presented a new analytical method of Raman ratio spectrum, which can extract the amide A band of proteins in water. To obtain the Raman ratio spectrum, the Raman spectrum of aqueous protein was divided by that of pure water. A mathematical simulation was employed to examine whether Raman ratio spectrum is effective. Two kinds of protein, lysozyme and （^-chymotrypsin were employed. The amide A bands of them in water were extracted from Raman ratio spectra. Additionally, the process of thermal denaturation of lysozyme was detected from Raman ratio spectrum. These results demonstrated the Raman ratio spectra could be employed to study the amide A modes of proteins in water.展开更多
基金This work was supported by the National Natural Science Foundation of China （No.91127042, No.21103158, No.21273211, No.21473171）, the National Key Basic Research Special Foundation （No.2013CB834602 and No.2010CB923300）, the Fundamental Research Funds for the Central Universities （No.7215623603）, and the Hua-shan Mountain Scholar Program. We also thank Doctor Kang-zhen Tian and Professor Shu-ji Ye for the measurement of IR spectra of aqueous lysozyme.
文摘The amide A band of protein is sensitive to the hydrogen bands of amide groups of proteins. However, it is hard to distinguish the amide A band of aqueous protein in situ directly, since it overlaps with O-H stretching vibration of water. In this work, we presented a new analytical method of Raman ratio spectrum, which can extract the amide A band of proteins in water. To obtain the Raman ratio spectrum, the Raman spectrum of aqueous protein was divided by that of pure water. A mathematical simulation was employed to examine whether Raman ratio spectrum is effective. Two kinds of protein, lysozyme and （^-chymotrypsin were employed. The amide A bands of them in water were extracted from Raman ratio spectra. Additionally, the process of thermal denaturation of lysozyme was detected from Raman ratio spectrum. These results demonstrated the Raman ratio spectra could be employed to study the amide A modes of proteins in water.
