[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results s...Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results showed that contents of soluble protein in the two species sharply declined 7 to 21 days after flowering, as the soluble sugar in Zhongmian 42 leveling off after 21 days of flowering while the soluble sugar in Xinluzao 36 dropped notably after 21 days of flowering before remaining stable after seven days later. The soluble sugar decreased 7 to 14 days after flowering before sharply rising to the maximum seven days later, and then began to decline quickly. The soluble sugar was the minimum after 35 days of flowering and then remaining stable. Peroxidase activity generally increased. Indole-3- acetic acid oxidase activities were low at 7 days after flowering. IAAO activity reached to the peaks on the 14th and 28th day after flowering. IAAO activity of two varieties decreased with the same trend 35 days after flowering.展开更多
Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from...Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from sera df normal lipidemic donors was separated bysequential ultracentrifugation. The separated human IDL 1 mg·mL^(-1) in phosphate buffer saline, pH7.4, was incubated with cupric sulfate (10 μmol·L^(-1) ) at 37℃ for 10 h in the presence orabsence of various concentrations of Iso. Malondialdehyde (MDA) formation, vitamin E consumption,electrophoretic mobility of LDL, mitochondria] membrane potential of mouse peritoneal macrophages,phagocytosis of neutral red, and release of nitric oxide (NO) from macrophages were determined byvarious methods. Results Iso 1 - 100 μmol·L^(-1) significantly inhibited the increase of MDAformation, vitamin E consumption and electrophoretic mobility of LDL induced by Cu^(2+) in aconcentration-dependent manner. The injury of the mitochondrial membrane potential of mouseperitoneal macrophages due to incubation with ox-LDL (0.1 mg·mL^(-1)) at 37℃ for 12 h was markedlyprotected by 10 μmol·L^(-1) Iso. After pretreat-ment of the macrophages with 10 μmol · L^(-1)of Iso and then exposure to ox-LDL for 4 h, the reduction of phagocytosis of neutral red and releaseof NO in response to lipopolysaccharide (IPS) stimulation were significantly prevented. ConclusionIso has protective action against Cu^(2+) - mediated LDL peroxidation and ox-LDL induced toxicity tomacrophages in vitro.展开更多
In order to carry out decolorization, sludge protein solution, a dark brown close to black solution from activated sludge, was subjected to ^(60)Co γ-ray irradiation in the presence of hydrogen peroxide. UV/Vis spect...In order to carry out decolorization, sludge protein solution, a dark brown close to black solution from activated sludge, was subjected to ^(60)Co γ-ray irradiation in the presence of hydrogen peroxide. UV/Vis spectrophotometric method was used to investigate the effect of H2O2 on the coloration apparent kinetics and rate constants of sludge protein solution under γ-ray irradiation. In addition, the effects of irradiation dose, initial sludge protein solution concentration, and pH value on the decolorization efficiency of sludge protein solution were studied. Results showed that the decolorization apparent kinetics of sludge protein solution was a first-order reaction. The solution decolorization percentage increased with the increase of irradiation dose or the decrease of initial sludge protein solution concentration. The examination results of pH value showed that the sludge protein solution could be more efficiently decolorized in alkaline media than in acid media. Moreover, sensory evaluation and foamability analysis indicated that irradiated samples under H2O2 oxidation showed better sensory score and foamability.展开更多
Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitum...Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.展开更多
Female-specific serum protein(FSSP)is normally present in the sera of female fish,but it is notfound in males.However,estradiol benzoate(EB)was found to induce the appearance of FSSP inmale fish and immature female fi...Female-specific serum protein(FSSP)is normally present in the sera of female fish,but it is notfound in males.However,estradiol benzoate(EB)was found to induce the appearance of FSSP inmale fish and immature female fish.Massive doses of FSSP caused a rapid increase in FSSP.Ultrastructural examination of the liver indicated an extensive proliferation of the rough endoplasmicreticulum and a decrease in glycogen and lipid droplets after EB injection.A preparative PAGE for isolating highly purified FSSP from the serum of EB-treated fish,Carassiusauratus cuvieri(Temminck & Schlegel),is described.Purified FSSP obtained from EB-induced O-crucian fish has a molecular weight of 466,000±4,000(n=10),while that in mature female serumis 480,000±40,000(n=2).FSSP appears to be a dimer,with the size of the possible monomerbeing 240,000±8,000(n=6).SDS-PAGE on gradient gels indicated that sera from males given multiple(12)injections of EBcontain a main band with a molecular weight of 147,000±6,000(n=6).However,the same serumsamples provided three bands of protein on the PAGE gels.Antiserum was raised against the electrophoretically purified FSSP.The resulting antibody formeda single,continuous precipitation line with sera from EB-treated males and vitellogenic females,butnot with that from normal males.lmmunocytochemistry(PAP method)was used to locate FSSP in the liver and ovary of maturefemales and the liver of EB-treated males.Strongly positive particles were found clustered in groupsaround liver cell nuclei under light microscopy,and the yolk granules in the oocytes were also filledwith positive particles.展开更多
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.
文摘Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results showed that contents of soluble protein in the two species sharply declined 7 to 21 days after flowering, as the soluble sugar in Zhongmian 42 leveling off after 21 days of flowering while the soluble sugar in Xinluzao 36 dropped notably after 21 days of flowering before remaining stable after seven days later. The soluble sugar decreased 7 to 14 days after flowering before sharply rising to the maximum seven days later, and then began to decline quickly. The soluble sugar was the minimum after 35 days of flowering and then remaining stable. Peroxidase activity generally increased. Indole-3- acetic acid oxidase activities were low at 7 days after flowering. IAAO activity reached to the peaks on the 14th and 28th day after flowering. IAAO activity of two varieties decreased with the same trend 35 days after flowering.
文摘Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from sera df normal lipidemic donors was separated bysequential ultracentrifugation. The separated human IDL 1 mg·mL^(-1) in phosphate buffer saline, pH7.4, was incubated with cupric sulfate (10 μmol·L^(-1) ) at 37℃ for 10 h in the presence orabsence of various concentrations of Iso. Malondialdehyde (MDA) formation, vitamin E consumption,electrophoretic mobility of LDL, mitochondria] membrane potential of mouse peritoneal macrophages,phagocytosis of neutral red, and release of nitric oxide (NO) from macrophages were determined byvarious methods. Results Iso 1 - 100 μmol·L^(-1) significantly inhibited the increase of MDAformation, vitamin E consumption and electrophoretic mobility of LDL induced by Cu^(2+) in aconcentration-dependent manner. The injury of the mitochondrial membrane potential of mouseperitoneal macrophages due to incubation with ox-LDL (0.1 mg·mL^(-1)) at 37℃ for 12 h was markedlyprotected by 10 μmol·L^(-1) Iso. After pretreat-ment of the macrophages with 10 μmol · L^(-1)of Iso and then exposure to ox-LDL for 4 h, the reduction of phagocytosis of neutral red and releaseof NO in response to lipopolysaccharide (IPS) stimulation were significantly prevented. ConclusionIso has protective action against Cu^(2+) - mediated LDL peroxidation and ox-LDL induced toxicity tomacrophages in vitro.
基金Supported by Foundation of Universities Double-Five Science and Technology Program of Tianjin(No.W20080003)
文摘In order to carry out decolorization, sludge protein solution, a dark brown close to black solution from activated sludge, was subjected to ^(60)Co γ-ray irradiation in the presence of hydrogen peroxide. UV/Vis spectrophotometric method was used to investigate the effect of H2O2 on the coloration apparent kinetics and rate constants of sludge protein solution under γ-ray irradiation. In addition, the effects of irradiation dose, initial sludge protein solution concentration, and pH value on the decolorization efficiency of sludge protein solution were studied. Results showed that the decolorization apparent kinetics of sludge protein solution was a first-order reaction. The solution decolorization percentage increased with the increase of irradiation dose or the decrease of initial sludge protein solution concentration. The examination results of pH value showed that the sludge protein solution could be more efficiently decolorized in alkaline media than in acid media. Moreover, sensory evaluation and foamability analysis indicated that irradiated samples under H2O2 oxidation showed better sensory score and foamability.
文摘Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.
文摘Female-specific serum protein(FSSP)is normally present in the sera of female fish,but it is notfound in males.However,estradiol benzoate(EB)was found to induce the appearance of FSSP inmale fish and immature female fish.Massive doses of FSSP caused a rapid increase in FSSP.Ultrastructural examination of the liver indicated an extensive proliferation of the rough endoplasmicreticulum and a decrease in glycogen and lipid droplets after EB injection.A preparative PAGE for isolating highly purified FSSP from the serum of EB-treated fish,Carassiusauratus cuvieri(Temminck & Schlegel),is described.Purified FSSP obtained from EB-induced O-crucian fish has a molecular weight of 466,000±4,000(n=10),while that in mature female serumis 480,000±40,000(n=2).FSSP appears to be a dimer,with the size of the possible monomerbeing 240,000±8,000(n=6).SDS-PAGE on gradient gels indicated that sera from males given multiple(12)injections of EBcontain a main band with a molecular weight of 147,000±6,000(n=6).However,the same serumsamples provided three bands of protein on the PAGE gels.Antiserum was raised against the electrophoretically purified FSSP.The resulting antibody formeda single,continuous precipitation line with sera from EB-treated males and vitellogenic females,butnot with that from normal males.lmmunocytochemistry(PAP method)was used to locate FSSP in the liver and ovary of maturefemales and the liver of EB-treated males.Strongly positive particles were found clustered in groupsaround liver cell nuclei under light microscopy,and the yolk granules in the oocytes were also filledwith positive particles.
