P-糖蛋白与药物的结合体在体内的转化过程探讨

目的ATP结合盒转运载体蛋白作为影响药物体内过程的重要因素已被广泛研究,P-糖蛋白(P-gp)是其中最主要的一种转运子。P-gp的结构、特点及组织分布决定了其在药物的吸收、分布、代谢、排泄方面的重要作用。了解P-gp的这些作用有助于增加临床用药的合理性。经过近三十年的发展,虽然研究P-gp的方法已经较为成熟;但是,目前对转运子的研究仍有许多争议存在,还有很多问题需要解决。本文主要阐述P-gp的特性及其对药物体内过程的影响。

Construction of Prokaryotic Expression Vectors of EBP1 Gene from Nervilia Fordii (Hance) Schltr.

[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.

完全抗原与半抗原有何不同？

答：具有免疫原性，同时又具有反应原性的物质称为完全抗原，如异种血清蛋白。只有反应原性而无免疫原性的物质称为半抗原或不完全抗原。半抗原分子量很小，本身没有免疫原性，但与蛋白载体结合后可获得免疫原性。载体通常是大分子蛋白质，它们能与做为抗原决定簇的半抗原分子结合，从而形成半抗原-载体复合物。这种复合物通常具有半抗原和载体蛋白特异的免疫原性，或只具有半抗原特异的免疫原性。如青霉素的降解产物青霉烯酸即为半抗原，与人体蛋白结合形成青霉烯酸蛋白而成为具有免疫原性的完全抗原。