Background： The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on ...Background： The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on the extraction method of soluble proteins for this species. The extraction is the most critical step in two-dimensional gel electrophoresis and each extraction method has its advantages, disadvantages and limitations. Therefore protein extraction methods should be optimized for each tissue before starting an experimental setup. In prospect of future DIGE （Differential Gel electrophoresis） experiments for the investigation of the effects of cadmium and inoculation with plant growth promoting bacteria at the proteome level, the aim of this study was to optimize an extraction method for soluble proteins of poplar leaves and roots. Results： The acetone-phenol extraction method was found to be the most suited, rendering a high spot number and low background interference. During further optimization, several critical steps in the extraction method were revealed. Conclusion： Aiming to optimize the extraction of soluble leaf and root proteins of Populus deltoides × （trichocarpa× deltoides） compatible with DIGE analysis, a protocol rendering high reproducibility, low background interference and a high spot number was established, however no novel insights were acquired.展开更多
AIM: To explore the inhibitory effects of pokeweed antiviral protein seed (PAP-S) and PAP encoded by a eukaryotic expression plasmid on hepatitis B virus (HBV) replication in vitro. METHODS: HepG2 2.2.15 cells in cult...AIM: To explore the inhibitory effects of pokeweed antiviral protein seed (PAP-S) and PAP encoded by a eukaryotic expression plasmid on hepatitis B virus (HBV) replication in vitro. METHODS: HepG2 2.2.15 cells in cultured medium were treated with different concentrations of PAP-S. HBsAg, HBeAg and HBV DNA in supernatants were determined by ELISA and fluorescent quantitative PCR respectively. MTT method was used to assay for cytotoxicity. HepG2 were cotransfected with various amounts of PAP encoded by a eukaryotic expression plasmid and replication competent wild-type HBV 1.3 fold over- length plasmid. On d 3 after transfection, HBsAg and HBeAg were determined by using ELISA. Levels of HBV core-associated DNA and RNA were detected by using Southern and Northern blot, respectively. RESULTS: The inhibitory effects of PAP-S on HBsAg, HBeAg and HBV DNA were gradually enhanced with the increase of PAP concentration. When the concentration of PAP-S was 10 μg/mL, the inhibition rates of HBsAg, HBeAg and HBV DNA were 20.9%, 30.2% and 50%, respectively. After transfection of 1.0 μg and 2.0 μg plasmid pXF3H-PAP, the levels of HBV nucleocapside- associated DNA were reduced by 38.0% and 74.0% respectively, the levels of HBsAg in the media by 76.8% and 99.7% respectively, and the levels of HBeAg by 72.7% and 99.3% respectively as compared with controls. Transfection with 2 μg plasmid pXF3H-PAP reduced the levels of HBV nucleocapside-associated RNA by 69.0%.CONCLUSION: Both PAP-S and PAP encoded by a eukaryotic expression plasmid could effectively inhibit HBV replication and antigen expression in vitro, and the inhibitory effects were dose-dependent.展开更多
Hepatic lipidosis is a common lesion in chelonians and may be related with vitellogenesis, hyperparathyroidism, follicular stasis, fatty diets or lacking nutrients, sedentary life and obesity, bacterial toxins in live...Hepatic lipidosis is a common lesion in chelonians and may be related with vitellogenesis, hyperparathyroidism, follicular stasis, fatty diets or lacking nutrients, sedentary life and obesity, bacterial toxins in liver and starvation. Clinical signs are unspecific. Routine biochemical tests don＇t have specificity and it is better defined through biopsy. The objective was evaluated the biochemical hepatic profile of red footed tortoises （Chelonoidis carbonaria） with the histopathological findings of lipidosis by laparoscopic biopsy. Samples of blood were collected for biochemistry of thirty-six animals from two different groups, twenty animals from a zoo and sixteen from a breeder. The animals were submitted to liver biopsy by videolaparoscopy. Three fragments were collected, two of them were processed by histology and one went to electron microscopy. Nineteen tortoises from the zoo had lipidosis in laparoscopy and macrovesicular degeneration. Fifteen tortoises from the breeder had microvesicular degeneration. The diagnosis of steatosis was confirmed by electron microscopy. There was no statistical difference of aspartate aminotransferase levels between the two groups; however there was significant difference of triglycerides levels. The enzymatic activity of transaminases is not related to the histological grading of lipidosis in tortoises, however triglycerides levels are higher in animals with more severe gradation.展开更多
An 8-week growth trial was conducted to determine dietary arginine requirement for juvenile Pacific white shrimp Litopenaeus vannamei （Boone） with an initial average body weight of 0.47 g reared in low-salinity wat...An 8-week growth trial was conducted to determine dietary arginine requirement for juvenile Pacific white shrimp Litopenaeus vannamei （Boone） with an initial average body weight of 0.47 g reared in low-salinity water （0.50-1.50 g L-1）. Six isonitrogenous and isoenergetic semipurified diets （crude protein 360 g/kg and gross energy 19.70 kJ/g） were formulated with fish meal and wheat gluten as the intact protein source.展开更多
Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years. Expressed sequence tag （EST） analysis was carried out for this dinoflagellate in order to identify the functional gene...Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years. Expressed sequence tag （EST） analysis was carried out for this dinoflagellate in order to identify the functional genes involved in its biological processes. A cDNA library was constructed for P. donghaiense at exponential growth phase, and 565 usable sequencing reads were obtained from 700 clones selected randomly. Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons （EST groups）. Twenty-two EST groups were found to tag the genes involved in diverse biological processes including programmed cell death （PCD）. Two EST groups showed significant homologies with the encoding genes of cysteine protease （caspase） and proliferating cell nuclear antigen, respectively, two key proteins involved in PCD.展开更多
OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to invest...OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to investigate the antiglycation and antioxidation potential of two medicinal plants:Juglans regia and Calendula officinalis.METHODS:In-vitro investigation was carried out to discover the antiglycation and antioxidation potential of J.regia and C.officinalis.Using an Ultraviolet Double-beam Spectrophotometer,we evaluated the antiglycation property of the crude methanolic extracts of J.regia and C.officinalis by assessing their ability to inhibit the Maillard reaction.Employing the same instrument we also measured the antioxidation potential of these plant extracts using the nitric oxide(NO) free radical-scavenging assay.RESULTS:J.regia had greater antiglycation ability,with a minimum inhibitory concentration(MIC 50) of 28 μg/mL as compared with that of C.officinalis(270 μg/mL).C.officinalis had greater antioxidation potential(26.10,22.07 and 16.06% at 0.5 mg,0.25 mg and 0.125 mg,respectively,as compared with 18.15,16.50 and 16.06% of J.regia,respectively).CONCLUSION:J.regia and C.officinalis inhibited the Maillard reaction and prevented oxidation in-vitro.Hence,the extracts of these plants could have therapeutic uses in curbing chronic diabetic complications and slowing down aging.展开更多
文摘Background： The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on the extraction method of soluble proteins for this species. The extraction is the most critical step in two-dimensional gel electrophoresis and each extraction method has its advantages, disadvantages and limitations. Therefore protein extraction methods should be optimized for each tissue before starting an experimental setup. In prospect of future DIGE （Differential Gel electrophoresis） experiments for the investigation of the effects of cadmium and inoculation with plant growth promoting bacteria at the proteome level, the aim of this study was to optimize an extraction method for soluble proteins of poplar leaves and roots. Results： The acetone-phenol extraction method was found to be the most suited, rendering a high spot number and low background interference. During further optimization, several critical steps in the extraction method were revealed. Conclusion： Aiming to optimize the extraction of soluble leaf and root proteins of Populus deltoides × （trichocarpa× deltoides） compatible with DIGE analysis, a protocol rendering high reproducibility, low background interference and a high spot number was established, however no novel insights were acquired.
基金the National Natural Sciences Foundation ofChina, No. 30671860
文摘AIM: To explore the inhibitory effects of pokeweed antiviral protein seed (PAP-S) and PAP encoded by a eukaryotic expression plasmid on hepatitis B virus (HBV) replication in vitro. METHODS: HepG2 2.2.15 cells in cultured medium were treated with different concentrations of PAP-S. HBsAg, HBeAg and HBV DNA in supernatants were determined by ELISA and fluorescent quantitative PCR respectively. MTT method was used to assay for cytotoxicity. HepG2 were cotransfected with various amounts of PAP encoded by a eukaryotic expression plasmid and replication competent wild-type HBV 1.3 fold over- length plasmid. On d 3 after transfection, HBsAg and HBeAg were determined by using ELISA. Levels of HBV core-associated DNA and RNA were detected by using Southern and Northern blot, respectively. RESULTS: The inhibitory effects of PAP-S on HBsAg, HBeAg and HBV DNA were gradually enhanced with the increase of PAP concentration. When the concentration of PAP-S was 10 μg/mL, the inhibition rates of HBsAg, HBeAg and HBV DNA were 20.9%, 30.2% and 50%, respectively. After transfection of 1.0 μg and 2.0 μg plasmid pXF3H-PAP, the levels of HBV nucleocapside- associated DNA were reduced by 38.0% and 74.0% respectively, the levels of HBsAg in the media by 76.8% and 99.7% respectively, and the levels of HBeAg by 72.7% and 99.3% respectively as compared with controls. Transfection with 2 μg plasmid pXF3H-PAP reduced the levels of HBV nucleocapside-associated RNA by 69.0%.CONCLUSION: Both PAP-S and PAP encoded by a eukaryotic expression plasmid could effectively inhibit HBV replication and antigen expression in vitro, and the inhibitory effects were dose-dependent.
文摘Hepatic lipidosis is a common lesion in chelonians and may be related with vitellogenesis, hyperparathyroidism, follicular stasis, fatty diets or lacking nutrients, sedentary life and obesity, bacterial toxins in liver and starvation. Clinical signs are unspecific. Routine biochemical tests don＇t have specificity and it is better defined through biopsy. The objective was evaluated the biochemical hepatic profile of red footed tortoises （Chelonoidis carbonaria） with the histopathological findings of lipidosis by laparoscopic biopsy. Samples of blood were collected for biochemistry of thirty-six animals from two different groups, twenty animals from a zoo and sixteen from a breeder. The animals were submitted to liver biopsy by videolaparoscopy. Three fragments were collected, two of them were processed by histology and one went to electron microscopy. Nineteen tortoises from the zoo had lipidosis in laparoscopy and macrovesicular degeneration. Fifteen tortoises from the breeder had microvesicular degeneration. The diagnosis of steatosis was confirmed by electron microscopy. There was no statistical difference of aspartate aminotransferase levels between the two groups; however there was significant difference of triglycerides levels. The enzymatic activity of transaminases is not related to the histological grading of lipidosis in tortoises, however triglycerides levels are higher in animals with more severe gradation.
文摘An 8-week growth trial was conducted to determine dietary arginine requirement for juvenile Pacific white shrimp Litopenaeus vannamei （Boone） with an initial average body weight of 0.47 g reared in low-salinity water （0.50-1.50 g L-1）. Six isonitrogenous and isoenergetic semipurified diets （crude protein 360 g/kg and gross energy 19.70 kJ/g） were formulated with fish meal and wheat gluten as the intact protein source.
基金Supported by the Key Laboratory of Mariculture of Chinese Education Ministry, Ocean University of China (No. 200405)the Ministry of Science and Technology of China (No. 2005CCA02400,2001CB409700)
文摘Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years. Expressed sequence tag （EST） analysis was carried out for this dinoflagellate in order to identify the functional genes involved in its biological processes. A cDNA library was constructed for P. donghaiense at exponential growth phase, and 565 usable sequencing reads were obtained from 700 clones selected randomly. Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons （EST groups）. Twenty-two EST groups were found to tag the genes involved in diverse biological processes including programmed cell death （PCD）. Two EST groups showed significant homologies with the encoding genes of cysteine protease （caspase） and proliferating cell nuclear antigen, respectively, two key proteins involved in PCD.
文摘OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to investigate the antiglycation and antioxidation potential of two medicinal plants:Juglans regia and Calendula officinalis.METHODS:In-vitro investigation was carried out to discover the antiglycation and antioxidation potential of J.regia and C.officinalis.Using an Ultraviolet Double-beam Spectrophotometer,we evaluated the antiglycation property of the crude methanolic extracts of J.regia and C.officinalis by assessing their ability to inhibit the Maillard reaction.Employing the same instrument we also measured the antioxidation potential of these plant extracts using the nitric oxide(NO) free radical-scavenging assay.RESULTS:J.regia had greater antiglycation ability,with a minimum inhibitory concentration(MIC 50) of 28 μg/mL as compared with that of C.officinalis(270 μg/mL).C.officinalis had greater antioxidation potential(26.10,22.07 and 16.06% at 0.5 mg,0.25 mg and 0.125 mg,respectively,as compared with 18.15,16.50 and 16.06% of J.regia,respectively).CONCLUSION:J.regia and C.officinalis inhibited the Maillard reaction and prevented oxidation in-vitro.Hence,the extracts of these plants could have therapeutic uses in curbing chronic diabetic complications and slowing down aging.
